Data of H2O2 release from AQP8-knockdown rat hepatocyte mitochondria

Danielli, Mauro; Marrone, Julieta; Capiglioni, Alejo M.; Marinelli, Raúl A.

doi:10.1016/j.dib.2019.103722

Cited by 8 publications

(6 citation statements)

References 3 publications

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“…As highlighted in the introduction section, S1R resides mainly at the interface mitochondrion associated membrane of the ER [24]. Moreover, AQP8 and AQP11 are localized in mitochondria [25,26] and ER [18] respectively, but ER can change is shape forming a reticular structure throughout the entire cytoplasm, thus enabling physical connections to other subcellular structures, such as the plasma membrane and mitochondria [53,54]. Results of co-localization experiments evidenced an intracellular localization of S1R [24] and AQP11 [32], while AQP3 and AQP8 were localized mainly on the plasma membrane.…”

Section: Discussionmentioning

confidence: 91%

“…S1R is a chaperone protein, mainly expressed in mitochondria-associated endoplasmic reticulum (ER) membranes [24]. Interestingly, it has been demonstrated that also AQP8 and AQP11 are localized in mitochondria [25,26] and ER [18], respectively and possess H 2 O 2 permeability.…”

Section: Introductionmentioning

confidence: 99%

“…Starting from the evidence that AQP8 and AQP11 are localized in mitochondria [25,26] and endoplasmic reticulum (ER) [18], respectively, and that S1R is also mainly expressed in mitochondria-associated ER membranes [24], in the present paper, we studied the potential of S1R modulators as AQPs interfering compounds. Briefly, we studied the in vitro effect of the well-established S1R agonists PRE084 and RC33, and of the S1R antagonist NE100, on AQPs.…”

Section: Introductionmentioning

confidence: 99%

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Sigma-1 Receptor Agonists Acting on Aquaporin-Mediated H2O2 Permeability: New Tools for Counteracting Oxidative Stress

Pellavio

Rossino

Gastaldi

et al. 2021

IJMS

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Sigma1 Receptor (S1R) is involved in oxidative stress, since its activation is triggered by oxidative or endoplasmic reticulum stress. Since specific aquaporins (AQP), called peroxiporins, play a relevant role in controlling H2O2 permeability and ensure reactive oxygen species wasted during oxidative stress, we studied the effect of S1R modulators on AQP-dependent water and hydrogen peroxide permeability in the presence and in the absence of oxidative stress. Applying stopped-flow light scattering and fluorescent probe methods, water and hydrogen peroxide permeability in HeLa cells have been studied. Results evidenced that S1R agonists can restore water permeability in heat-stressed cells and the co-administration with a S1R antagonist totally counteracted the ability to restore the water permeability. Moreover, compounds were able to counteract the oxidative stress of HeLa cells specifically knocked down for S1R. Taken together these results support the hypothesis that the antioxidant mechanism is mediated by both S1R and AQP-mediated H2O2 permeability. The finding that small molecules can act on both S1R and AQP-mediated H2O2 permeability opens a new direction toward the identification of innovative drugs able to regulate cell survival during oxidative stress in pathologic conditions, such as cancer and degenerative diseases.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sigma-1 Receptor Agonists Acting on Aquaporin-Mediated H2O2 Permeability: New Tools for Counteracting Oxidative Stress

Pellavio

Rossino

Gastaldi

et al. 2021

IJMS

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“…Interestingly, mammalian AQP8 appears to have the largest H 2 O 2 permeability ( Bienert et al, 2007 ). Genetic manipulation of mitochondrial AQP8 supports its role in H 2 O 2 release to the cytosol ( Danielli et al, 2019a , b ), which could contribute to Cd-induced cell death.…”

Section: And Kidney Mitochondrial Damagementioning

confidence: 93%

Iron and Cadmium Entry Into Renal Mitochondria: Physiological and Toxicological Implications

Thévenod

Lee

Garrick

2020

Front. Cell Dev. Biol.

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Regulation of body fluid homeostasis is a major renal function, occurring largely through epithelial solute transport in various nephron segments driven by Na + /K + -ATPase activity. Energy demands are greatest in the proximal tubule and thick ascending limb where mitochondrial ATP production occurs through oxidative phosphorylation. Mitochondria contain 20–80% of the cell’s iron, copper, and manganese that are imported for their redox properties, primarily for electron transport. Redox reactions, however, also lead to reactive, toxic compounds, hence careful control of redox-active metal import into mitochondria is necessary. Current dogma claims the outer mitochondrial membrane (OMM) is freely permeable to metal ions, while the inner mitochondrial membrane (IMM) is selectively permeable. Yet we recently showed iron and manganese import at the OMM involves divalent metal transporter 1 (DMT1), an H + -coupled metal ion transporter. Thus, iron import is not only regulated by IMM mitoferrins, but also depends on the OMM to intermembrane space H + gradient. We discuss how these mitochondrial transport processes contribute to renal injury in systemic (e.g., hemochromatosis) and local (e.g., hemoglobinuria) iron overload. Furthermore, the environmental toxicant cadmium selectively damages kidney mitochondria by “ionic mimicry” utilizing iron and calcium transporters, such as OMM DMT1 or IMM calcium uniporter, and by disrupting the electron transport chain. Consequently, unraveling mitochondrial metal ion transport may help develop new strategies to prevent kidney injury induced by metals.

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“…Moving from these considerations, and prompted by the evidences that AQP8 and AQP11, are localized in mitochondria [42,43] and ER [15], respectively, in the present paper we studied the potential of S1R modulators as AQPs interfering compounds. As a first step we studied the in vitro effect of the well-established S1R agonists PRE-084 and RC-33 (both in its racemic and (R)-configured form), and of the S1R antagonist NE-100, on AQPs.…”

Section: Introductionmentioning

confidence: 97%

Dual Aquaporin and Sigma1 Receptor (DAS) Modulators: New Tools for Counteracting Oxidative Stress

Pellavio¹,

Rossino²,

Gastaldi³

et al. 2020

Preprint

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Specific aquaporins (AQP), called peroxyporins, play a relevant role in controlling H2O2 permeability and ensure reactive oxygen species wasting during oxidative stress. Another target involved in oxidative stress is the Sigma1 Receptor (S1R), since its activation is triggered by oxidative or endoplasmic reticulum stress. Herein we evaluated the effect of S1R modulators on AQP-dependent water permeability in the presence and in the absence of oxidative stress. Applying stopped-flow light scattering and fluorescent probe methods, water and hydrogen peroxide permeability in Hela cells have been studied. Results evidenced that S1R agonists can restore water permeability in heat-stressed cells and the co-administration with a S1R antagonist totally counteracted the ability to restore the water permeability. All compounds except one were able to counteract the oxidative stress of HeLa cells specifically knocked down for S1R. Taken together, our results support the hypothesis that the investigated compounds act as dual aquaporin and Sigma1 receptor (DAS) modulators. The finding that small molecules can modulate both AQP and S1R opens a new direction toward the identification of innovative drugs able to regulate cell survival during oxidative stress in pathologic conditions, like cancer and degenerative diseases.

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Data of H2O2 release from AQP8-knockdown rat hepatocyte mitochondria

Cited by 8 publications

References 3 publications

Sigma-1 Receptor Agonists Acting on Aquaporin-Mediated H2O2 Permeability: New Tools for Counteracting Oxidative Stress

Sigma-1 Receptor Agonists Acting on Aquaporin-Mediated H2O2 Permeability: New Tools for Counteracting Oxidative Stress

Iron and Cadmium Entry Into Renal Mitochondria: Physiological and Toxicological Implications

Dual Aquaporin and Sigma1 Receptor (DAS) Modulators: New Tools for Counteracting Oxidative Stress

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