This study presented the removal of methylene blue in the batch system based on heterogeneous and homogenous catalytic ozonation. The effect of different variables such as initial (methylene blue) MB concentration, solution pH (3–11), contact time (5–40 min), and catalyst dosage on homogeneous and heterogeneous ozonation systems was examined. The findings of this research showed that MB removal was better in alkaline conditions. The amount of removed MB in acidic conditions was slightly better than in neutral conditions (approximately 10–15%). In addition, the removal efficiency of MB was decreased by increasing of initial concentration. Besides, as the amount of catalyst increased, the removal efficiency had been increased by 40%. The significant difference between the use of two processes of single ozonation processes (SOP) and catalytic ozonation processes (COPs) was due to the use of catalysts. Based on the findings, MB degradation in COPs was several times faster than SOP (approximately 4.7 times). It was due to the numerous advantages of these processes in the removal of environmental pollutants, they can be widely used in the removal of various pollutants if their cost can be reduced.