Data Processing System (DPS) software with experimental design, statistical analysis and data mining developed for use in entomological research

Tang, Qisheng; Zhang, Chuan‐Xi

doi:10.1111/j.1744-7917.2012.01519.x

Cited by 1,020 publications

(605 citation statements)

References 23 publications

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“…The plates were measured at A 470 at 25°C every 5 min for 2 h using a Varioskan Flash multimode reader. Statistical analyses were performed using Data Processing System (DPS) v13.5 (69). Comparisons of two samples were conducted using Student's t test.…”

Section: Methodsmentioning

confidence: 99%

A Venom Serpin Splicing Isoform of the Endoparasitoid Wasp Pteromalus puparum Suppresses Host Prophenoloxidase Cascade by Forming Complexes with Host Hemolymph Proteinases

Yan

Fang

Liu

et al. 2017

Journal of Biological Chemistry

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Edited by Charles E. SamuelTo ensure successful parasitism, parasitoid wasps inject venom along with their eggs into their hosts. The venom serves to suppress host immune responses, including melanization. Venom from Pteromalus puparum, a pupal endoparasitoid, inhibits melanization of host hemolymph in vitro in a dose-dependent manner. Using assay-guided fractionation, a serpin splicing isoform with phenoloxidase inhibitory activity was identified as P. puparum serpin-1, venom isoform (PpS1V). This serpin gene has 16 predicted splicing isoforms that differ only in the C-terminal region. RT-PCR results show that the specific serpin isoform is differentially expressed in the venom gland. Recombinant PpS1V (rPpS1V) suppresses host prophenoloxidase (PPO) activation rather than inhibiting the phenoloxidase directly. Pulldown assays show that PpS1V forms complexes with two host hemolymph proteins, here named Pieris rapae hemolymph proteinase 8 (PrHP8) and P. rapae prophenoloxidase-activating proteinase 1 (PrPAP1), based on gene sequence blasting and phylogenetic analysis. The role of rPrPAP1 in the PPO activation cascade and its interaction with rPpS1V were confirmed. The stoichiometry of inhibition of PrPAP1 by PpS1V is 2.3. PpS1V also inhibits PPO activation in a non-natural host, Ostrinia furnacalis, through forming a complex with O. furnacalis serine protease 13 (OfSP13), an ortholog to PrPAP1. Our results identify a venom-enriched serpin isoform in P. puparum that inhibits host PPO activation, probably by forming a complex with host hemolymph proteinase PrPAP1.

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Section: Methodsmentioning

confidence: 99%

A Venom Serpin Splicing Isoform of the Endoparasitoid Wasp Pteromalus puparum Suppresses Host Prophenoloxidase Cascade by Forming Complexes with Host Hemolymph Proteinases

Yan

Fang

Liu

et al. 2017

Journal of Biological Chemistry

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“…Based on the above ETM bands, multiple SVM inverse model was established, and the parameters of the model were chosen by GA. Because of the limited samples, K-folding cross test was applied as follows: the samples set were separated into 7 mutually non-intersect subsets randomly; chose seven samples and six subsets for the last subsets as alternative training set, and the last one was utilized for validation; library file LIBSVM was employed to construct the SVM [23] and the running platform were carried out by DPS software [24]. The previous data of six points are used to construct the model and the other seven data to test.…”

Section: Resultsmentioning

confidence: 99%

GA-SVM Applied in Assessing the Water Trophic State of South Lake Qujiang based on Multispectral RS

Huo¹,

Zheng²,

Wang³

et al. 2017

J Environ Anal Toxicol

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“…All statistical analyses were done using the DPS © package (version 8.01 for windows) (Tang and Feng, 2007). To analyze the predatory capacity of different Class of P. sinensis on GAS in both choice and no-choice trials, data were subjected to one-way analyses of variance (ANOVA) with repeated measures and Tukey's multiple comparison methods.…”

Section: Methodsmentioning

confidence: 99%

Biological control of golden apple snail, Pomacea canaliculata by Chinese soft-shelled turtle, Pelodiscus sinensis in the wild rice, Zizania latifolia field

Dong

Zheng

et al. 2012

Sci. agric. (Piracicaba, Braz.)

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The wild rice, Zizania latifolia Turcz, used to be one of the important aquatic vegetables cultivated in China. Recently, the golden apple snail -GAS (Pomacea canaliculata (Lamarck)) was found to be a major invasive pest attacking Z. latifolia. To control efficiently GAS, predation by the Chinese soft-shelled turtles (Pelodiscus sinensis) on GAS was evaluated in laboratory and field trials. P. sinensis had a strong predatory capacity and selectivity for GAS both in laboratory and field conditions. All the sizes of P. sinensis prefer to capture smaller snails. The optimum number of P. sinensis released in Z. latifolia field was dependent on the density of over-wintered GAS, and varied between 30 and 50 turtles per 666.7 m 2 . The number of GAS declined in the fields with turtles as compared to turtle-free field. A pattern of releasing P. sinensis in Z. latifolia fields was developed and widely adopted by farmers because of much more benefit besides biologically controlling GAS.

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Data Processing System (DPS) software with experimental design, statistical analysis and data mining developed for use in entomological research

Cited by 1,020 publications

References 23 publications

A Venom Serpin Splicing Isoform of the Endoparasitoid Wasp Pteromalus puparum Suppresses Host Prophenoloxidase Cascade by Forming Complexes with Host Hemolymph Proteinases

A Venom Serpin Splicing Isoform of the Endoparasitoid Wasp Pteromalus puparum Suppresses Host Prophenoloxidase Cascade by Forming Complexes with Host Hemolymph Proteinases

GA-SVM Applied in Assessing the Water Trophic State of South Lake Qujiang based on Multispectral RS

Biological control of golden apple snail, Pomacea canaliculata by Chinese soft-shelled turtle, Pelodiscus sinensis in the wild rice, Zizania latifolia field

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