Data set of Aspergillus flavus induced alterations in tear proteome: Understanding the pathogen-induced host response to fungal infection

Kandhavelu, Jeyalakshmi; Demonte, Naveen Luke; Namperumalsamy, Venkatesh Prajna; Lalitha, Prajna; Thangavel, Chitra; Jayapal, Jeya Maheshwari; Dharmalingam, Kuppamuthu

doi:10.1016/j.dib.2016.11.003

Cited by 7 publications

(3 citation statements)

References 9 publications

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“…Neutrophil extracellular trap formation by neutrophils has been reported to protect against corneal infection by Pseudomonas and Aspergillus on the ocular surface [ 153 , 154 , 155 ]. Infectious keratitis is a severe disease that can threaten vision.…”

Section: Neutrophils On the Ocular Surfacementioning

confidence: 99%

Role of Neutrophils on the Ocular Surface

Mun

Hwang

Shin

2021

IJMS

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The ocular surface is a gateway that contacts the outside and receives stimulation from the outside. The corneal innate immune system is composed of many types of cells, including epithelial cells, fibroblasts, natural killer cells, macrophages, neutrophils, dendritic cells, mast cells, basophils, eosinophils, mucin, and lysozyme. Neutrophil infiltration and degranulation occur on the ocular surface. Degranulation, neutrophil extracellular traps formation, called NETosis, and autophagy in neutrophils are involved in the pathogenesis of ocular surface diseases. It is necessary to understand the role of neutrophils on the ocular surface. Furthermore, there is a need for research on therapeutic agents targeting neutrophils and neutrophil extracellular trap formation for ocular surface diseases.

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Section: Neutrophils On the Ocular Surfacementioning

confidence: 99%

Role of Neutrophils on the Ocular Surface

Mun

Hwang

Shin

2021

IJMS

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“…Neutrophils can kill the pathogenic bacteria by phagocytosis, degranulation, and/or NETs formation as the front line of host defense [ 53 , 54 ]. Many reports suggest that NETs formation by neutrophils protects against corneal infection by Pseudomonas and Aspergillus on the ocular surface [ 55 , 56 , 57 ]. Pseudomonas aeruginosa forms biofilm, which is a prominent source of bacterial keratitis with aggressive and swiftly developing attributes.…”

Section: Netosis As Host Defense On Ocular Surfacementioning

confidence: 99%

Neutrophil Extracellular Traps and Their Possible Implications in Ocular Herpes Infection

Kapoor

Shukla

2023

Pathogens

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Neutrophil extracellular traps (NETs) are net-like structures released from neutrophils. NETs predominantly contain cell-free deoxyribonucleic acid (DNA) decorated with histones and neutrophil granule proteins. Numerous extrinsic and intrinsic stimuli can induce the formation of NETs such as pathogens, cytokines, immune complexes, microcrystals, antibodies, and other physiological stimuli. The mechanism of NETosis induction can either be ROS-dependent or independent based on the catalase producing activity of the pathogen. NADPH is the source of ROS production, which in turn depends on the upregulation of Ca2+ production in the cytoplasm. ROS-independent induction of NETosis is regulated through toll-like receptors (TLRs). Besides capturing and eliminating pathogens, NETs also aggravate the inflammatory response and thus act as a double-edged sword. Currently, there are growing reports of NETosis induction during bacterial and fungal ocular infections leading to different pathologies, but there is no direct report suggesting its role during herpes simplex virus (HSV) infection. There are innumerable independent reports showing that the major effectors of NETosis are also directly affected by HSV infection, and thus, there is a strong possibility that HSV interacts with these facilitators that can either result in virally mediated modulation of NETosis or NETosis-mediated suppression of ocular HSV infection. This review focuses on the mechanism of NETs formation during different ocular pathologies, with its prime focus on highlighting their potential implications during HSV ocular infections and acting as prospective targets for the treatment of ocular diseases.

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“…The Proteome Discoverer program version 2.2 (Thermo Fisher Scientific, Waltham, MA, USA) was used to create a peak list. Only proteins with peptide spectrum matches (PSMs) higher than three were used for data analysis [74]. The resulting intact and fragmentation pattern was compared to a theoretical fragmentation pattern using the UniProt database for S. pasteurii [75] in order to identify proteins based on the peptides present.…”

Section: Proteomics Analysismentioning

confidence: 99%

Effects of Soil Surface Chemistry on Adsorption and Activity of Urease from a Crude Protein Extract: Implications for Biocementation Applications

Vilar

Ikuma

2022

Catalysts

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In the bacterial enzyme-induced calcite precipitation (BEICP) technique for biocementation, the spatial distribution of adsorbed and catalytically active urease dictates the location where calcium carbonate precipitation and resulting cementation will occur. This study investigated the relationships between the amount of urease and total bacterial proteins adsorbed, the retained enzymatic activity of adsorbed urease, and the overall loss of activity upon adsorption, and how these relationships are influenced by changes in soil surface chemistry. In soils with hydrophobic contents higher than 20% (w/w) ratio, urease was preferentially adsorbed compared to the total amount of proteins present in the crude bacterial protein extract. Conversely, adsorption of urease onto silica sand and soil mixtures, including iron-coated sand, was much lower compared to the total proteins. Higher levels of urease activity were retained in hydrophobic-containing samples, with urease activity decreasing with lower hydrophobic content. These observations suggest that the surface manipulation of soils, such as treatments to add hydrophobicity to soil surfaces, can potentially be used to increase the activity of adsorbed urease to improve biocementation outcomes.

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Data set of Aspergillus flavus induced alterations in tear proteome: Understanding the pathogen-induced host response to fungal infection

Cited by 7 publications

References 9 publications

Role of Neutrophils on the Ocular Surface

Role of Neutrophils on the Ocular Surface

Neutrophil Extracellular Traps and Their Possible Implications in Ocular Herpes Infection

Effects of Soil Surface Chemistry on Adsorption and Activity of Urease from a Crude Protein Extract: Implications for Biocementation Applications

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