Datura metel: In Vitro Production of Tropane Alkaloids

Cusidó, Rosa M.; Palazón, Javier; Mt, Piñol; Bonfill, Mercedes; Morales, Catalina Rivas

doi:10.1055/s-1999-13976

Cited by 31 publications

(17 citation statements)

References 9 publications

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“…The use of a vertical mesh support within a reactor as an inoculum stand by other investigators has shown itself to be an efficient method for distributing roots and promoting radial growth (9,18). In this work, replacing the glass draft by a mesh that imposes a similar hydrodynamic response gave an advantage for allowing growth all around the reactor and better inoculation of root tissue in the downcomer, not limiting fluids recirculation.…”

Section: Discussionmentioning

confidence: 85%

Novel Airlift Reactor Fitting for Hairy Root Cultures: Developmental and Performance Studies

Caspeta

Quintero

Villarreal

2008

Biotechnol Progress

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In vitro growth of Solanum chrysotrichum hairy roots was carried out in three different types of reactors: shake flasks, a glass-draught internal-loop 2-L basic design airlift reactor (BDR), and a novel modified mesh-draught with wire-helixes 2-L reactor (MR). In each of them, the growth patterns were different, as well as some of the dynamic parameters. The specific growth rates were 0.08, 0.067, and 0.112 d(-1) for shake flasks, BDR, and MR, respectively. In shake flasks and in the MR, growth followed first-order kinetics. In the MR without roots, superficial liquid velocity in the riser and downcomer ranged from 2.1 to 2.7 and 1.4 to 1.7 cm s(-1), respectively (nearly the same as the BDR values). After 42 days in culture, tissue density in the MR was twice that found in the BDR and about the same as that found in the shake flasks. At the tissue densities reached at 42 days, superficial liquid velocities in the MR and BDR downcomers were 4-5 and 7-8 times lower, and mixing times were 11 and 18 times longer than those observed without roots. Tissue densities measured at three points in the MR's downcomer and riser ranged from 10.21 to 12.17 and 4.94 to 5.24 gDW L(-1) respectively. Dynamic gas hold-up dropped faster when roots grew radially in the mesh-draught. In addition, root cultures were scaled-up in a 10-L MR reactor in which some geometric relations were maintained, such as the Q/V radio. Growth in 10-L MR followed first-order kinetics, but despite this, specific growth velocity was 0.09 d(-1) and overall tissue density diminished slightly with respect to that of the 2-L MR. Tissue inoculation, distribution, and harvest were more easily accomplished in the MRs.

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Section: Discussionmentioning

confidence: 85%

Novel Airlift Reactor Fitting for Hairy Root Cultures: Developmental and Performance Studies

Caspeta

Quintero

Villarreal

2008

Biotechnol Progress

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“…Similarly, Boitel-Conti et al have shown that Tween 20 (polyoxyethylene sorbitan monolaurate) stimulates the release of tropane alkaloids from Datura innoxia Mill. hairy root cultures [3], and this effect was confirmed with Datura metel [6]. Tween is widely used in microbial and animal biotechnologies, but this is its first use in plants [3].…”

Section: Introductionmentioning

confidence: 76%

Up-Regulation of Licochalcone A Biosynthesis and Secretion by Tween 80 in Hairy Root Cultures of Glycyrrhiza uralensis Fisch

Zhang

Liu²,

Chen³

et al. 2010

Mol Biotechnol

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We evaluated the effect of Tween 80 as elicitor on licochalcone A from hairy root cultures of Glycyrrhiza uralensis Fisch. After a 15-days treatment with 2% Tween 80, hairy roots still grew well and produced higher levels of licochalcone A and total flavonoids than the control (without treatment). Licochalcone A content and total flavonoid content were 3.103 and 127.095 mg per flask (9- and 11-fold higher), respectively, compared with controls. Secretion of licochalcone A and total flavonoids into the culture medium was remarkably high, up to 98 and 94% of the total production, respectively. The enhanced flavonoid production was associated with elevated mRNA levels and enzyme activities of phenylalanine ammonia-lyase (PAL), 4-coumarate:coenzyme A ligase (4CL), and cinnamate-4-hydroxylase (C4H). These results clearly demonstrated that Tween 80 treatment permeabilized the roots to enhance secretion, but also acted as an efficient elicitor of licochalcone A and total flavonoid production in hairy roots of G. uralensis Fisch.

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“…The main active constituents of the plant are the medicinally important tropane alkaloids such as hyoscyamine and scopolamine (Cusidó et al, 1999). Due to the presence of bioactive compounds, the plant was widely used in traditional medicine to cure diseases such as asthma, cough, wound treatment, convulsion, headache, insanity, hemorrhoids, and rheumatism Dabur et al, 2004).…”

mentioning

confidence: 99%

First Report of Leaf Spot of Datura metel Caused by Alternaria tenuissima in Korea

Aktaruzzaman

Kim

Afroz

et al. 2015

Research in Plant Disease

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In June 2013, we collected leaf spot disease samples of Datura metel from Gangneung, Gangwon Province, Korea. The symptoms observed were small circular to oval dark brown spots with irregular in shape or remained circular with concentric rings. We isolated the pathogen from infected leaves and cultured the fungus on potato dextrose agar. We examined the fungus morphologically and confirmed its pathogenicity according to Koch's postulates. The results of morphological examinations, pathogenicity tests, and the rDNA sequences of the internal transcribed spacer regions (ITS1 and ITS4), glycerol-3-phosphate dehydrogenase (G3PDH) and the RNA polymerase II second largest subunit (RPB2) gene sequence revealed that the causal agent was Alternaria tenuissima. To the best of our knowledge, this is the first report of leaf spot of D. metel caused by A. tenuissima in Korea as well as worldwide.

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Datura metel: In Vitro Production of Tropane Alkaloids

Cited by 31 publications

References 9 publications

Novel Airlift Reactor Fitting for Hairy Root Cultures: Developmental and Performance Studies

Novel Airlift Reactor Fitting for Hairy Root Cultures: Developmental and Performance Studies

Up-Regulation of Licochalcone A Biosynthesis and Secretion by Tween 80 in Hairy Root Cultures of Glycyrrhiza uralensis Fisch

First Report of Leaf Spot of Datura metel Caused by Alternaria tenuissima in Korea

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