Day–night cycles and the sleep-promoting factor, Sleepless, affect stem cell activity in the
            <i>Drosophila</i>
            testis

Tulina, Natalia; Chen, Wenfeng; Chen, Jung Hsuan; Sowcik, Mallory; Sehgal, Amita

doi:10.1073/pnas.1316552111

Cited by 18 publications

(12 citation statements)

References 51 publications

(75 reference statements)

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“…For genes implicated in regulating sleep, temporal requirements vis-à-vis sleep have been assessed chiefly in an adult-specific manner using the Geneswitch system. While our results confirm that the panneuronally expressed elav-GS transgene is compatible with adult-specific manipulations as reported previously ( Yuan et al 2006 ; Joiner et al 2006 ; Seugnet et al 2008 , 2011 ; Bushey et al 2009 ; Donlea et al 2009 ; Wu et al 2009 ; Kuo et al 2010 , 2012 ; Crocker et al 2010 ; Ishimoto and Kitamoto 2010 ; Pfeiffenberger and Allada 2012 ; Erion et al 2012 ; Ueno et al 2012 ; Vanderheyden et al 2013 ; Tulina et al 2014 ; Liu et al 2014 ; Kayser et al 2014 ; Oh et al 2014 ; Chen et al 2015 ; Tabuchi et al 2015 ; Dissel et al 2015 ; Afonso et al 2015 ), they indicate that developmental elav-GS induction elicits strong, nonspecific alterations of sleep later in adulthood. Notably, these sleep deficits occur in animals exposed to low concentrations (11.6 µM) of RU486, nearly 50-fold below those typically used for adult manipulations (500 µM).…”

Section: Discussionsupporting

confidence: 92%

“…More recently, photosensitive transcriptional activators have been described ( Chan et al 2015 ). Of these strategies, the steroid-inducible Geneswitch system ( Osterwalder et al 2001 ; Roman et al 2001 ) has been the most frequently employed to study sleep and has been used in nearly two dozen such studies to date ( Yuan et al 2006 ; Joiner et al 2006 ; Seugnet et al 2008 , 2011 ; Bushey et al 2009 ; Donlea et al 2009 ; Wu et al 2009 ; Kuo et al 2010 , 2012 ; Crocker et al 2010 ; Ishimoto and Kitamoto 2010 ; Pfeiffenberger and Allada 2012 ; Erion et al 2012 ; Ueno et al 2012 ; Vanderheyden et al 2013 ; Tulina et al 2014 ; Liu et al 2014 ; Kayser et al 2014 ; Oh et al 2014 ; Chen et al 2015 ; Tabuchi et al 2015 ; Dissel et al 2015 ; Afonso et al 2015 ).…”

mentioning

confidence: 99%

“…Nearly all of the reported uses of Geneswitch to manipulate sleep in Drosophila have utilized adult-specific induction, achieved by feeding RU486-containing food to adult flies ( Yuan et al 2006 ; Joiner et al 2006 ; Seugnet et al 2008 , 2011 ; Bushey et al 2009 ; Donlea et al 2009 ; Wu et al 2009 ; Kuo et al 2010 , 2012 ; Crocker et al 2010 ; Ishimoto and Kitamoto 2010 ; Pfeiffenberger and Allada 2012 ; Erion et al 2012 ; Ueno et al 2012 ; Vanderheyden et al 2013 ; Tulina et al 2014 ; Liu et al 2014 ; Kayser et al 2014 ; Oh et al 2014 ; Chen et al 2015 ; Tabuchi et al 2015 ; Dissel et al 2015 ; Afonso et al 2015 ). RU486 is well tolerated in adults with no detectable toxic effects at high concentrations (500 µM) ( Osterwalder et al 2001 ; Roman et al 2001 ), and studies of sleep have typically used concentrations at or below this threshold to activate Geneswitch transgenes expressed neuronally ( elav-GS ) ( e.g.…”

mentioning

confidence: 99%

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Evaluation of Ligand-Inducible Expression Systems for Conditional Neuronal Manipulations of Sleep inDrosophila

Stavropoulos

2016

G3 Genes|Genomes|Genetics

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Drosophila melanogaster is a powerful model organism for dissecting the molecular mechanisms that regulate sleep, and numerous studies in the fly have identified genes that impact sleep–wake cycles. Conditional genetic analysis is essential to distinguish the mechanisms by which these genes impact sleep: some genes might exert their effects developmentally, for instance by directing the assembly of neuronal circuits that regulate sleep; other genes may regulate sleep in adulthood; and yet other genes might influence sleep by both developmental and adult mechanisms. Here we have assessed two ligand-inducible expression systems, Geneswitch and the Q-system, for conditional and neuronally restricted manipulations of sleep in Drosophila. While adult-specific induction of a neuronally expressed Geneswitch transgene (elav-GS) is compatible with studies of sleep as shown previously, developmental induction of elav-GS strongly and nonspecifically perturbs sleep in adults. The alterations of sleep in elav-GS animals occur at low doses of Geneswitch agonist and in the presence of transgenes unrelated to sleep, such as UAS-CD8-GFP. Furthermore, developmental elav-GS induction is toxic and reduces brood size, indicating multiple adverse effects of neuronal Geneswitch activation. In contrast, the transgenes and ligand of the Q-system do not significantly impact sleep–wake cycles when used for constitutive, developmental, or adult-specific neuronal induction. The nonspecific effects of developmental elav-GS activation on sleep indicate that such manipulations require cautious interpretation, and suggest that the Q-system or other strategies may be more suitable for conditional genetic analysis of sleep and other behaviors in Drosophila.

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Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of Ligand-Inducible Expression Systems for Conditional Neuronal Manipulations of Sleep inDrosophila

Stavropoulos

2016

G3 Genes|Genomes|Genetics

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“…In our experiments, we counted each GSC and based the P-value on the percentage of pHH3-positive GSCs out of the total number of GSCs. Other studies calculated the P-value based on numbers of dividing GSCs per numbers of testes (Boyle et al, 2007;Chen et al, 2008;Inaba et al, 2011;Tulina et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

G-Protein signaling accelerates stem cell divisions in Drosophila males

Malpe

Kudyba

et al. 2018

Preprint

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Adult stem cells divide to renew the stem cell pool and replenish specialized cells that are lost due to death or usage. However, little is known about the mechanisms regulating how stem cells adjust to a demand for specialized cells. A failure of the stem cells to respond to this demand can have serious consequences, such as tissue loss, or prolonged recovery post injury.Here, we challenge the male germline stem cells (GSCs) of Drosophila melanogaster for the production of specialized cells using mating experiments. We show that repeated mating reduced the sperm pool and accelerated germline stem cell (GSC) divisions. The increase in GSC divisions depended on the activity of the highly conserved G-proteins. Germline expression of RNA-Interference (RNA-i) constructs against G-proteins or a dominant negative G-protein eliminated the increase in GSC divisions in mated males. Consistent with a role for the G-proteins in the regulation of GSC divisions, RNA-i against seven out of 35 G-protein coupled receptors (GPCRs) within the germline cells also eliminated the capability of males to accelerate their GSC divisions in response to mating. Our data show that GSCs are receptive to GPCR stimulus, potentially through a network of interactions among multiple signaling pathways.

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“…Control of the cell cycle progression by the circadian clock is known as circadian gating of the cell cycle. Previous reports have indicated that the CR system regulates cell cycle progress both at the G1/S (Geyfman et al, 2012; Kowalska et al, 2013) and at G2/M (Matsuo et al, 2003; Tulina et al, 2014) transitions. In contrast, the cell cycle can regulate the circadian clock as well.…”

Section: Common Synchronization Methods Used In Labmentioning

confidence: 99%

New Insights Into the Circadian Rhythm and Its Related Diseases

et al. 2019

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Circadian rhythms (CR) are a series of endogenous autonomous oscillators generated by the molecular circadian clock which acting on coordinating internal time with the external environment in a 24-h daily cycle. The circadian clock system is a major regulatory factor for nearly all physiological activities and its disorder has severe consequences on human health. CR disruption is a common issue in modern society, and researches about people with jet lag or shift works have revealed that CR disruption can cause cognitive impairment, psychiatric illness, metabolic syndrome, dysplasia, and cancer. In this review, we summarized the synchronizers and the synchronization methods used in experimental research, and introduced CR monitoring and detection methods. Moreover, we evaluated conventional CR databases, and analyzed experiments that characterized the underlying causes of CR disorder. Finally, we further discussed the latest developments in understanding of CR disruption, and how it may be relevant to health and disease. Briefly, this review aimed to synthesize previous studies to aid in future studies of CR and CR-related diseases.

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Day–night cycles and the sleep-promoting factor, Sleepless, affect stem cell activity in the Drosophila testis

Cited by 18 publications

References 51 publications

Evaluation of Ligand-Inducible Expression Systems for Conditional Neuronal Manipulations of Sleep inDrosophila

Evaluation of Ligand-Inducible Expression Systems for Conditional Neuronal Manipulations of Sleep inDrosophila

G-Protein signaling accelerates stem cell divisions in Drosophila males

New Insights Into the Circadian Rhythm and Its Related Diseases

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