Daya Hambat Ekstrak Daun Sirsak, Kirinyuh, dan Rimpang Lengkuas terhadap Pertumbuhan Koloni Colletotrichum acutatum

Abstract

ABSTRAKTumbuhan yang berpotensi sebagai pestisida nabati, diantaranya sirsak (Annona muricata), kirinyuh (Choromoleana odorata) dan lengkuas (Alpinia galanga). Tujuan penelitian ini adalah menguji efektivitas ekstrak daun sirsak, kirinyuh dan rimpang lengkuas terhadap pertumbuhan koloni Colletotrichum acutatum, penyebab penyakit antraknosa pada tanaman cabai, secara in vitro. Penelitian dilaksanakan di laboratorium Fakultas Pertanian Universitas Siliwangi mulai bulan Juli sampai Agustus 2016. Rancangan Percobaan menggunakan Rancangan Acak Lengkap yang terdiri dari 9 perlakuan dengan 3 ulangan. Perlakuan terdiri dari : A = kontrol; B = ekstrak daun sirsak 0,5%; C = ekstrak daun sirsak 1%; D = ekstrak daun kirinyuh 0,5%; E = ekstrak daun kirinyuh 1%; F = ekstrak rimpang lengkuas 0,5%; G = ekstrak rimpang lengkuas 1%; H = campuran ekstrak daun sirsak, kirinyuh dan rimpang lengkuas 0,5%; dan I = campuran ekstrak daun sirsak, kirinyuh dan rimpang lengkuas 1%. Hasil penelitian menunjukkan bahwa campuran ekstrak daun sirsak, kirinyuh dan rimpang lengkuas 1% efektif dalam menghambat pertumbuhan koloni C.acutatum, pada masa inkubasi 7 hari sebesar 66,19% dan pada masa inkubasi 14 hari sebesar 69,94%. Ketiga ekstrak pestisida nabati tersebut memiliki potensi sebagai anti jamur C. acutatum.Kata Kunci : Antraknosa, Cabai, Pestisida nabati ABSTRACT Several plants that are potentially used as bio-pesticides are soursop, siam weed, and galangal. The research objective was to find out the effectiveness of leaf extract of soursop and C. odorata, and extract of galangal rhizome in inhibiting the growth of Colletotrichum acutatum colonies, causing antracnose on chilli, in in vitro. The experiment was conducted in the laboratory of Agriculture Faculty, Universitas Siliwangi Tasikmalaya from July until August 2016. The research design used was a completely randomized design consisted of nine treatments and three replications. The treatments were A ( control); B (soursop leaf extract, 0,5%); C (soursop leaf extract, 1%); D (C. odorata leaf extract, 0,5 %); E (C. odorata leaf extract 1%); F (galangal rhizome extract 0,5%); G (galangal rhizome extract 1%); H (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 0,5%; and I (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 1%). The results

Inhibitory Effect of Syzygium aromaticum L. Essential Oil Against the Fungal Pathogens of Capsicum annuum L.

“…Phatogenecity test was carried out by different methods adjusting to phatogen nature to explore phatogenic potential of the isolated that used in this study. The investigation used the method of Ivey et al, Dagani & Ceramica, and Hodiyah et al [2,17,18] with slight modifications. Inoculate pepper fruits by 20µl of the C. acutatum conidial suspension on the surface injured site.…”

“…The pathogenicity test was conducted to determine the pathogenicity potency of the strain. This test also aims to prove that the pathogen shows the same symptoms as the symptoms in the field [18]. The result of the pathogenicity test is shown in Table 2.…”

The effectiveness of the mixture of basil and betel leaves methanol extracts to suppress Colletotrichum acutatum, the causal agent of anthracnose disease on postharvest cayenne chili

“…In addition to being applied singly, bio fungicides can also be used by mixing two types of organic materials, thereby increasing efficiency and effectiveness and minimizing the occurrence of resistance (Akhtar and Isman 2013). The mixture of soursop leaf extract (Anona muricata L.), siam weeds leaves (Chromolaena odorata L.), and galangal rhizomes (Alpinia galanga L.) at a concentration of 1% successfully inhibit the growth of C. acutatum colonies in cayenne chili with an incubation period of seven days and inhibitory percentage of 66.19% (Hodiyah et al 2017). A total of 1% betel leaf methanol extract mixed with 2% Andrographis paniculata 2% can suppress the development of the diameter and biomass of Colletotrichum gloeosporioides colonies by 91.26% (Idris and Nurmansyah 2015).…”