Daya Hidup Spermatozoa Epididimis Sapi Persilangan yang Dipreservasi dengan Air Kelapa Muda pada Suhu 5oC (VIABILITY OF EPIDIDYMAL SPERMATOZOA CROSSBREED CATTLE PRESERVED WITH COCONUT WATER AT 5oC)

Rizal, Muhammad; Riyadhi, Muhammad; Irawan, Bambang; Wahdi, Anis; Habibah, Habibah; Herdis,

doi:10.19087/jveteriner.2017.18.4.571

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…After incubation, take one drop and place it on an object glass, then cover it with a cover glass and observe using a microscope with a magnification of 400×. Observations were performed on the typical changes that occurred, i.e., the presence of a coiled tail tip which identified the integrity of the plasma membrane (Indriani et al, 2013). The calculation was carried out by counting the intact plasma membrane of the total number of spermatozoa observed.…”

Section: Plasma Membrane Integrity Evaluationmentioning

confidence: 99%

Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years

Purnawan,

Rimayanti,

Susilowati

et al. 2023

J. Med. Vet.

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The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p < 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.

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Section: Plasma Membrane Integrity Evaluationmentioning

confidence: 99%

Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years

Purnawan,

Rimayanti,

Susilowati

et al. 2023

J. Med. Vet.

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“…Spermatozoa yang hidup ditandai oleh kepala tidak berwarna atau transparan sedangkan yang mati ditandai oleh kepala berwarna merah. Sebanyak 200 spermatozoa diamati dengan mikroskop cahaya menggunakan perbesaran 400x [14].…”

Section: B Persentase Daya Hidup Spermatozoaunclassified

“…Berdasarkan data kualitas spermatozoa kauda epidimis pada Tabel 1 di atas, dapat diketahui bahwa spermatozoa mencit (M. musculus L.) yang akan digunakan penelitian memiliki kualitas yang baik dan memenuhi syarat untuk dilakukan preservasi. Hal ini sesuai dengan indikator spermatozoa yang baik yaitu persentase motilitas dan daya hidup adalah ˃ 70% [20], persentase abnormalitas adalah ˂ 30% [21] serta konsentrasi spermatozoa kauda epididimis pada hewan mamalia berkisar 10-50 juta sel/ml [14].…”

Section: Pengamatan Kualitas Spermatozoa Sebelum Proses Pengenceranunclassified

The The Effect of Giving The Antioxidant Vitamin C in Tris-Egg Yolk Extender During The Preservation Period on The Quality of The Epididymal Spermatozoa of Mice (Mus musculus L.)

Dewi,

Mahriani

2023

B. SAINSTEK

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Spermatozoa preservation is a method used to store spermatozoa in a diluent solution such as NaCl or tris-egg yolk. However, the longer storage of spermatozoa in the diluent solution can generally form ROS (Reactive Oxygen Species). The addition of antioxidants such as vitamin C to the diluent is expected to be able to bind ROS so that they are not toxic to spermatozoa and sperm quality can be maintained during the preservation period. The purpose of this study was to determine the effect of giving the antioxidant vitamin C in tris-egg yolk diluent during the preservation period on the quality of mice epididymal spermatozoa. Vitamin C used in this study was 0.5 mg, 1 mg, and 2 mg in 100 ml of tris-egg yolk diluent. Observation of spermatozoa quality was carried out within 0 to 48 hours with a span of 12 hours. The results showed that the addition of Vitamin C concentrations of 0.5 mg, 1 mg, and 2 mg in 100 ml of tris-egg yolk diluent was able to maintain the percentage of motility and vitality, but the longer the storage the quality of spermatozoa decreased. The concentration of vitamin C 0.5 mg, 1 mg, and 2 mg in 100 ml of tris-egg yolk diluent is a good concentration to maintain the quality of spermatozoa during 12 hours of storage, the rest will decrease. Further research is needed to increase the storage time of spermatozoa by using vitamin C and tris-egg yolk.

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“…Rata-rata motilitas dan daya hidup pada penelitian ini masing-masing adalah 71% dan 84%, dimana nilai ini sedikit rendah dengan penelitian terdahulu pada sapi persilangan sebesar 72% (motilitas) dan 85% (daya hidup) (Rizal et al, 2017) serta pada sapi bali sebesar 75% (motilitas) dan 86,75% (daya hidup) (Labetubun & Siwa, 2011). Menurut Patel et al (2017) karakteristik semen seekor pejantan yang mempunyai tingkat reproduksi yang baik memiliki nilai motilitas >60%.…”

Section: Karakteristik Spermatozoa Segar Kauda Epididimisunclassified

Motilitas Dan Daya Hidup Spermatozoa Asal Epididimis Sapi Persilangan yang Diencerkan dengan Air Tebu

Riyadhi

Rizal²,

Thahir³

2020

jitro

Self Cite

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ABSTRAKAir tebu diketahui mengandung sukrosa yang dapat meningkatkan energi bagi motilitas spermatozoa. Penelitian ini bertujuan untuk mengetahui kemampuan air tebu dan kuning telur dalam mempertahankan kualitas spermatozoa asal kauda epididymis sapi persilangan. Penelitian ini menggunakan epididimis sapi persilangan dari rumah pemotongan hewan (RPH) Basirih Kotamadya Banjarmasin. Spermatozoa yang dikoleksi ditempatkan ke dalam empat tabung reaksi masing-masing terdiri dari: 80% pengencer laktosa + 20% kuning telur ayam ras (kontrol), 45% air tebu + 45% akubidestilata + 10% kuning telur ayam ras (ATKT10), 42,5% air tebu + 42,5% akubidestilata + 15% kuning telur ayam ras (ATKT15), dan 40% air tebu + 40% akubidestilata + 20% kuning telur ayam ras (ATKT20). Parameter pengamatan adalah motilitas, daya hidup, dan membran plasma utuh. Hasil penelitian menunjukkan motilitas pengencer kontrol (65%), berbeda nyata (P<0,05) dengan ATKT10 (49%), ATKT15 (48%) dan ATKT20 (54%), namun demikian ATKT20 masih lebih tinggi (P<0,05) dengan ATKT10 dan ATKT15. Persentase daya hidup spermatozoa berbeda nyata (P<0,05) antara kontrol (79,60%) dengan ATKT10 (72,40%), ATKT15 (73,20%), dan ATKT20 (74,60%), akan tetapi ATKT20 dan ATKT15 masih lebih baik (P<0,05) dari ATKT10. Untuk persentase MPU, kontrol sebesar 76,4%, sama dengan (P>0,05) perlakuan ATKT 20 sebesar 76%, akan tetapi persentase MPU kontrol masih lebih baik (P<0,05) dari ATKT 10 sebesar 71,80% dan ATKT15 sebesar 74,20%. Dari hasil penelitian ini, terlihat kemampuan air tebu mampu mempertahankan motilitas spermatozoa sampai dengan dua hari.Kata Kunci : air tebu, sapi persilangan, spermatozoa asal kauda epididimisABSTRACT Sugarcane juice is known to contain sucrose which can increase energy for spermatozoa motility. The object of the study was to determine the ability of sugarcane juice and egg yolk in maintaining the quality of spermatozoa from cauda epididymis of crossbred cattle. This study used epididymal of crossing cattle from the slaughterhouse of Basirih - Banjarmasin. The collected spermatozoa were divided into four test tubes with the same volume in according to the number of treatments i.e. : 80% lactose + 20% egg yolk (control), 45% sugarcane + 45% aquabidest +10% egg yolk (ATKT10), 42.5% sugarcane + 42.5% aquabidest +15% egg yolk (ATKT15), and 40% sugarcane + 40% aquabidest +20% egg yolk (ATKT20). The parameter observed included percentage of motility, viability and intact plasma membrane (IPM). The results showed that (1) the percentage of motility (65%) and spermatozoa viability (79.60%) of control were higher (P <0.05) than in other treatments, however, ATKT20 had a higher percentage of both parameters than in ATKT10 and ATKT15; (2) the percentage of IPM control (76,4%) was not significantly different (P>0,05) to ATKT20 (76%). Both treatments have higher of IPM than ATKT10 (71.80%) and ATKT15 (74.20%). The sugarcane juice was able to maintain spermatozoa motility for up to two days.Keywords: crossbred cattle, spermatozoa from cauda epididymis, sugarcane juice

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Daya Hidup Spermatozoa Epididimis Sapi Persilangan yang Dipreservasi dengan Air Kelapa Muda pada Suhu 5oC (VIABILITY OF EPIDIDYMAL SPERMATOZOA CROSSBREED CATTLE PRESERVED WITH COCONUT WATER AT 5oC)

Cited by 4 publications

References 15 publications

Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years

Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years

The The Effect of Giving The Antioxidant Vitamin C in Tris-Egg Yolk Extender During The Preservation Period on The Quality of The Epididymal Spermatozoa of Mice (Mus musculus L.)

Motilitas Dan Daya Hidup Spermatozoa Asal Epididimis Sapi Persilangan yang Diencerkan dengan Air Tebu

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