DAZL binds to 3′UTR of Tex19.1 mRNAs and regulates Tex19.1 expression

Zeng, Mei; Lu, Yilu; Liao, Xinyang; Li, Dan; Sun, Huaqin; Liang, Shuli; Zhang, Sizhong; Ma, Yongxin; Yang, Zhipeng

doi:10.1007/s11033-009-9470-1

Cited by 20 publications

(15 citation statements)

References 24 publications

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“…It is unlikely that this regulatory mechanism functions in mature mouse oocytes in view of the recent reports that miRNA function is inactivated at this stage of oocyte development (Ma et al 2010;Suh et al 2010). At variance with our finding that both 39UTR mutagenesis and MO downregulation of DAZL decrease Tex19.1 translation, a study suggested that Dazl represses Tex19.1 translation when introduced in zebrafish oocytes (Zeng et al 2009). Although removal of DAZL elements from the 39UTR of Tex19.1 impairs accumulation of the reporter in MII oocytes, it also causes a small increase in reporter translation in GV, suggesting that this element may mediate translational repression at this stage.…”

Section: Discussioncontrasting

confidence: 94%

Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition

Chen¹,

et al. 2011

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Oocyte maturation, fertilization, and early embryonic development occur in the absence of gene transcription. Therefore, it is critical to understand at a global level the post-transcriptional events that are driving these transitions. Here we used a systems approach by combining polysome mRNA profiling and bioinformatics to identify RNA-binding motifs in mRNAs that either enter or exit the polysome pool during mouse oocyte maturation. Association of mRNA with the polysomes correlates with active translation. Using this strategy, we identified highly specific patterns of mRNA recruitment to the polysomes that are synchronized with the cell cycle. A large number of the mRNAs recovered with translating ribosomes contain motifs for the RNA-binding proteins DAZL (deleted in azoospermia-like) and CPEB (cytoplasmic polyadenylation element-binding protein). Although a Dazl role in early germ cell development is well established, no function has been described during oocyte-to-embryo transition. We demonstrate that CPEB1 regulates Dazl post-transcriptionally, and that DAZL is essential for meiotic maturation and embryonic cleavage. In the absence of DAZL synthesis, the meiotic spindle fails to form due to disorganization of meiotic microtubules. Therefore, Cpeb1 and Dazl function in a progressive, self-reinforcing pathway to promote oocyte maturation and early embryonic development.

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Section: Discussioncontrasting

confidence: 94%

Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition

Chen¹,

et al. 2011

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“…Numerous studies have identified putative mRNA targets of DAZL [10,11,42-46], however only a limited number of these have been validated in vivo [9,10]. In contrast, little is known about the identity of the mRNA targets of BOLL [47], and whether the motif to which it binds is similar to the GU-rich sequences bound by DAZL remains unclear [48].…”

Section: Discussionmentioning

confidence: 99%

A Developmental Stage-Specific Switch from DAZL to BOLL Occurs during Fetal Oogenesis in Humans, but Not Mice

Stewart

Kinnell

et al. 2013

PLoS ONE

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The Deleted in Azoospermia gene family encodes three germ cell-specific RNA-binding proteins (DAZ, DAZL and BOLL) that are essential for gametogenesis in diverse species. Targeted disruption of Boll in mice causes male-specific spermiogenic defects, but females are apparently fertile. Overexpression of human BOLL promotes the derivation of germ cell-like cells from genetically female (XX), but not male (XY) human ES cells however, suggesting a functional role for BOLL in regulating female gametogenesis in humans. Whether BOLL is expressed during oogenesis in mammals also remains unclear. We have therefore investigated the expression of BOLL during fetal oogenesis in humans and mice. We demonstrate that BOLL protein is expressed in the germ cells of the human fetal ovary, at a later developmental stage than, and almost mutually-exclusive to, the expression of DAZL. Strikingly, BOLL is downregulated, and DAZL re-expressed, as primordial follicles form, revealing BOLL expression to be restricted to a narrow window during fetal oogenesis. By quantifying the extent of co-expression of DAZL and BOLL with markers of meiosis, we show that this window likely corresponds to the later stages of meiotic prophase I. Finally, we demonstrate that Boll is also transiently expressed during oogenesis in the fetal mouse ovary, but is simultaneously co-expressed within the same germ cells as Dazl. These data reveal significant similarities and differences between the expression of BOLL homologues during oogenesis in humans and mice, and raise questions as to the validity of the Boll-/- mouse as a model for understanding BOLL function during human oogenesis.

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“…In most cases, DAZL acts by inducing translational activation of its target mRNAs, but its binding site, a GUU triplet, has not been reported to convey cytoplasmic polyadenylation in Xenopus oocytes . In zebrafish embryos, DAZL binding regulates the stability of its target mRNAs, presumably to restrict their expression to the future germ cells, as this protein is normally germ cell specific . In one such study, DAZL was reported to overcome miR‐430 mediated destabilization by the 3′ UTR of its own mRNA.…”

Section: Cytoplasmic Polyadenylation Of Mrnas In Germ Cells and Embryosmentioning

confidence: 80%

Specificity factors in cytoplasmic polyadenylation

2013

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Poly(A) tail elongation after export of an messenger RNA (mRNA) to the cytoplasm is called cytoplasmic polyadenylation. It was first discovered in oocytes and embryos, where it has roles in meiosis and development. In recent years, however, has been implicated in many other processes, including synaptic plasticity and mitosis. This review aims to introduce cytoplasmic polyadenylation with an emphasis on the factors and elements mediating this process for different mRNAs and in different animal species. We will discuss the RNA sequence elements mediating cytoplasmic polyadenylation in the 3′ untranslated regions of mRNAs, including the CPE, MBE, TCS, eCPE, and C-CPE. In addition to describing the role of general polyadenylation factors, we discuss the specific RNA binding protein families associated with cytoplasmic polyadenylation elements, including CPEB (CPEB1, CPEB2, CPEB3, and CPEB4), Pumilio (PUM2), Musashi (MSI1, MSI2), zygote arrest (ZAR2), ELAV like proteins (ELAVL1, HuR), poly(C) binding proteins (PCBP2, αCP2, hnRNP-E2), and Bicaudal C (BICC1). Some emerging themes in cytoplasmic polyadenylation will be highlighted. To facilitate understanding for those working in different organisms and fields, particularly those who are analyzing high throughput data, HUGO gene nomenclature for the human orthologs is used throughout. Where human orthologs have not been clearly identified, reference is made to protein families identified in man. © 2013 John Wiley & Sons, Ltd.

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DAZL binds to 3′UTR of Tex19.1 mRNAs and regulates Tex19.1 expression

Cited by 20 publications

References 24 publications

Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition

Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition

A Developmental Stage-Specific Switch from DAZL to BOLL Occurs during Fetal Oogenesis in Humans, but Not Mice

Specificity factors in cytoplasmic polyadenylation

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