DCE MRI reveals early decreased and later increased placenta perfusion after a stress challenge during pregnancy in a mouse model

Remus, Chressen Catharina; Kording, Fabian; Arck, Petra C.; Solano, Emilia; Sedlacik, Jan; Adam, Gerhard; Hecher, Kurt; Forkert, Nils D.

doi:10.1016/j.placenta.2018.03.009

Cited by 7 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such adverse effects on pregnancy have also been reported in animal models. For example, in pregnant mice, exposure to noise reduced placental blood flow, [15], impaired fetal growth [16], and programmed offspring for HPA dysfunction in adulthood [17]. Despite these findings, recent epidemiological reports suggest that air pollutant exposure may act as a confounding factor in the relationship between noise and health outcomes given their colocalization, making it difficult to understand the true influence of gestational noise exposure on fetal health [18, 19].…”

Section: Introductionmentioning

confidence: 99%

Fetal growth outcomes following peri-implantation exposure of Long-Evans rats to noise and ozone differ by sex

et al. 2019

View full text Add to dashboard Cite

BackgroundExposure to air pollution and high levels of noise have both been independently associated with the development of adverse pregnancy outcomes including low birth weight. However, exposure to such environmental stressors rarely occurs in isolation and is often co-localized, especially in large urban areas.MethodsThe purpose of this study was to compare the effects of combined exposure to noise (N) or ozone (O3), compared to either exposure alone. Long-Evans dams were exposed to air or 0.4 ppm ozone for 4 h on gestation day (GD) 5 and 6, coinciding with implantation receptivity. A subset of dams from each exposure group was further exposed to intermittent white noise (~ 85 dB) throughout the dark cycle following each inhalation exposure (n = 14 − 16/group). Uterine artery ultrasound was performed on GD 15 and 21. Fetal growth characteristics and indicators of placental nutrient status were measured at GD 21.ResultsExposure to ozone + quiet (O3 + Q) conditions reduced uterine arterial resistance at GD 15 compared to air + quiet (A + Q) exposure, with no further reduction by GD 21. By contrast, exposure to air + noise (A + N) significantly increased uterine arterial resistance at both GD 15 and 21. Notably, while peri-implantation exposure to O3 + Q conditions reduced male fetal weight at GD 21, this effect was not observed in the air + noise (A + N) or the ozone + noise (O3 + N) exposure groups. Fetal weight in female offspring was not reduced by ozone exposure alone (O3 + Q), nor was it affected by air + noise (A + N) or by combined ozone + noise (O3 + N) exposure.ConclusionsThese data indicate that exposure to ozone and noise differentially impact uterine blood flow, particularly at mid-gestation, with only ozone exposure being associated with sex-dependent fetal growth retardation in male offspring.

show abstract

Section: Introductionmentioning

confidence: 99%

Fetal growth outcomes following peri-implantation exposure of Long-Evans rats to noise and ozone differ by sex

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Administration via common mouse iv administration routes like femoral, jugular, or retro orbital veins is terminal and cannot be used for longitudinal monitoring. Although tail-vein injection (38, 43–45) can be performed multiple times, quantitative DCE requires administration of Gd contrast agent via an IV catheter inside the magnet during DCE acquisition, which is difficult for multiple administration longitudinally. Therefore, most mouse placental DCE studies with the steepest slope model were performed with single imaging time points.…”

Section: Resultsmentioning

confidence: 99%

“…The identification of separate perfusion chambers in the mouse placenta via DCE-based MRI has been shown in several other studies that correlate the perfusion chambers with the decidua and labyrinth (39)(40)(41). These zones are anatomically and physiologically defined and separated by a junction-protein dense membrane to regulate endocrine crosstalk between the two regions (54).…”

Section: Physiological Thresholding Of the Mouse Placenta Into Perfus...mentioning

confidence: 91%

See 1 more Smart Citation

Modeling Normal Mouse Uterine Contraction and Placental Perfusion with Non-invasive Longitudinal Dynamic Contrast Enhancement MRI

Cortes,

Stapleton,

Schwab

et al. 2024

Preprint

View full text Add to dashboard Cite

The placenta is a transient organ critical for fetal development. Disruptions of normal placental functions can impact health throughout an individual's entire life. Although being recognized by the NIH Human Placenta Project as an important organ, the placenta remains understudied, partly because of a lack of non-invasive tools for longitudinally evaluation for key aspects of placental functionalities. Non-invasive imaging that can longitudinally probe murine placental health in vivo are critical to understanding placental development throughout pregnancy. We developed advanced imaging processing schemes to establish functional biomarkers for non-invasive longitudinal evaluation of placental development. We developed a dynamic contrast enhancement magnetic resonance imaging (DCE-MRI) pipeline combined with advanced image process methods to model uterine contraction and placental perfusion dynamics. Our novel imaging pipeline uses subcutaneous administration of gadolinium for steepest-slope based perfusion evaluation. This enables non-invasive longitudinal monitoring. Additionally, we advance the placental perfusion chamber paradigm with a novel physiologically-based threshold model for chamber localization and demonstrate spatially varying placental chambers using multiple functional metrics that assess mouse placental development and continuing remodeling throughout gestation. Lastly, using optic flow to quantify placental motions arisen from uterine contractions in conjunction with time-frequency analysis, we demonstrated that the placenta exhibited asymmetric contractile motion.

show abstract

“…[14] Notably, trophectoderm cells from blastocysts cultured in vitro showed major changes in gene expression, including the activation of stress-related pathways and the downregulation of genes involved in placentation. [15] Furthermore, in human placentas after IVF-ET, genome-wide mRNA expression analysis identified the overexpression of genes involved in metabolism, immune response, transmembrane signaling, and cell cycle control. [16] Similarly, transcriptome data in mouse placental tissues showed that some IVF techniques may trigger the induction of genes involved in cellular proliferation and cell cycle progression.…”

Section: Introductionmentioning

confidence: 99%

Alterations in complement and coagulation pathways of human placentae subjected to in vitro fertilization and embryo transfer in the first trimester

et al. 2019

View full text Add to dashboard Cite

The mechanisms underlying the potential risks of in vitro fertilization and embryo transfer (IVF-ET) have not been fully elucidated. The aim of this study was to explore changes in the complement and coagulation pathways in placentae subjected to IVF-ET in the first trimester compared to placentae from normal pregnancies. Four placenta samples in the first trimester were obtained from patients undergoing IVF-ET owing to oviductal factors only. An additional 4 control placentae were obtained from volunteers with normal pregnancies. A GeneChip Affymetrix HG-U133 Plus 2.0 Array was utilized to analyze the changes in gene expression between the normal and IVF-ET placentae. Differentially expressed genes (DEGs) were analyzed using the Database for Annotation and Visualization and Integrated Discovery bioinformatics resource, and gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted. Using real-time PCR, we confirmed the obtained microarray data in 10 dysregulated genes. Five of the gene products were further analyzed by immunohistochemistry (IHC) to determine their protein expression and localization. A total of fifty DEGs were identified in the complement and coagulation pathways in the IVF-ET treated placentae: 38 upregulated and 12 down-regulated. KEGG pathway analysis indicated that IVF-ET manipulation substantially overactivated the coagulation and complement pathways, while urokinase plasminogen activator-and urokinase plasminogen activator receptor-mediated trophoblastic invasion and tissue remodeling were inhibited. Furthermore, the 5 proteins analyzed by IHC were found to be localized specifically to the placenta. This is the first study to compare DEGs relating to the placental complement and coagulation pathways from patients undergoing IVF-ET treatment compared to those undergoing normal pregnancy. These findings identified valuable biomarkers and potential novel therapeutic targets to combat the unfavorable effects of IVF-ET. Abbreviations: CD59 = CD59 molecule complement regulatory protein, CFD = complement factor D (adipsin), DAVID = Database for Annotation, Visualization and Integrated Discovery, DEG = differentially expressed gene, FGA = fibrinogen alpha chain, FGB = fibrinogen beta chain, FGG = fibrinogen gamma chain, GCOS = gene chip operating software, GEO = Gene Expression Omnibus, GO = gene ontology, IHC = immunohistochemistry, IVF-ET = in vitro fertilization and embryo transfer, PLAU = plasminogen activator urokinase, PLAUR = plasminogen activator urokinase receptor, PROC = protein C, qRT-PCR = quantitative real-time PCR, SERPINC1 = serpin peptidase inhibitor clade C member 1, SERPINE1 = serpin peptidase inhibitor clade E member 1, STRING = search tool for the retrieval of interacting genes, uPA = urokinase plasminogen activator, uPAR = urokinase plasminogen activator receptor.

show abstract

DCE MRI reveals early decreased and later increased placenta perfusion after a stress challenge during pregnancy in a mouse model

Cited by 7 publications

References 28 publications

Fetal growth outcomes following peri-implantation exposure of Long-Evans rats to noise and ozone differ by sex

Fetal growth outcomes following peri-implantation exposure of Long-Evans rats to noise and ozone differ by sex

Modeling Normal Mouse Uterine Contraction and Placental Perfusion with Non-invasive Longitudinal Dynamic Contrast Enhancement MRI

Alterations in complement and coagulation pathways of human placentae subjected to in vitro fertilization and embryo transfer in the first trimester

Contact Info

Product

Resources

About