De novo assembly of<i>Amorpha fruticosa</i>L. transcriptome in response to drought stress provides insight into the tolerance mechanisms

SUN, Xinzhu; Hu, Songmiao; Wang, Xin; Li, He; Zhou, Yun; Guan, Qing

doi:10.7717/peerj.11044

Cited by 7 publications

(5 citation statements)

References 52 publications

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“…The metabolic process may contribute to the cold tolerance in the northward invasion of Alternanthera philoxeroides [105]. The oxidation-reduction process is involved in the drought stress response in Amorpha fruticosa seedlings [106] and in the adaptation to hypoxia and low temperatures in Kandelia obovata [107]. Effective responses to environmental changes or stress stimuli are beneficial for the colonization and adaptation of invasive plants in nonindigenous areas.…”

Section: Role Of Adaptive Genes In M Micranthamentioning

confidence: 99%

Population Genomics Reveals Gene Flow and Adaptive Signature in Invasive Weed Mikania micrantha

Ruan

Wang

et al. 2021

Genes

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A long-standing and unresolved issue in invasion biology concerns the rapid adaptation of invaders to nonindigenous environments. Mikania micrantha is a notorious invasive weed that causes substantial economic losses and negative ecological consequences in southern China. However, the contributions of gene flow, environmental variables, and functional genes, all generally recognized as important factors driving invasive success, to its successful invasion of southern China are not fully understood. Here, we utilized a genotyping-by-sequencing approach to sequence 306 M. micrantha individuals from 21 invasive populations. Based on the obtained genome-wide single nucleotide polymorphism (SNP) data, we observed that all the populations possessed similar high levels of genetic diversity that were not constrained by longitude and latitude. Mikania micrantha was introduced multiple times and subsequently experienced rapid-range expansion with recurrent high gene flow. Using FST outliers, a latent factor mixed model, and the Bayesian method, we identified 38 outlier SNPs associated with environmental variables. The analysis of these outlier SNPs revealed that soil composition, temperature, precipitation, and ecological variables were important determinants affecting the invasive adaptation of M. micrantha. Candidate genes with outlier signatures were related to abiotic stress response. Gene family clustering analysis revealed 683 gene families unique to M. micrantha which may have significant implications for the growth, metabolism, and defense responses of M. micrantha. Forty-one genes showing significant positive selection signatures were identified. These genes mainly function in binding, DNA replication and repair, signature transduction, transcription, and cellular components. Collectively, these findings highlight the contribution of gene flow to the invasion and spread of M. micrantha and indicate the roles of adaptive loci and functional genes in invasive adaptation.

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Section: Role Of Adaptive Genes In M Micranthamentioning

confidence: 99%

Population Genomics Reveals Gene Flow and Adaptive Signature in Invasive Weed Mikania micrantha

Ruan

Wang

et al. 2021

Genes

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“…Four-week-sized seedlings of A. fruticosa were grown hydroponically in a 20% PEG 6000 (polyethylene glycol 6,000) solution for 72 h. Whole seedlings were sampled separately from the stressed and unstressed treatments, rapidly frozen in liquid nitrogen, and stored in a − 80°C refrigerator. After transcriptome sequencing results were obtained ( Sun et al, 2021 ), co-expression networks of genes were constructed using the WGCNA (Weighted Gene Co-expression Network Analysis) R package.…”

Section: Methodsmentioning

confidence: 99%

“…NAC-like transcription factors are one of the largest families of transcription factors specific to plants, and they play an important role in response to various abiotic stresses, such as salt, drought, and damage. In herbaceous plants such as Arabidopsis thaliana ( Mahmood et al, 2019 ), rice ( Li, 2014 ), alfalfa ( Shen et al, 2014 ), and land cotton ( Shah, 2013 ), NAC transcription factors were found to be upregulated in response to abiotic stresses, and in woody plants such as tamarisk ( Lu et al, 2019 ) and poplar ( Wang et al, 2015 ), PEG-6000 mimics drought stress; the transcriptome detected upregulation of NAC-like transcription factors in response to drought-induced differences in gene expression ( Sun et al, 2021 ). The NAC family has been found to function in a variety of processes, including apical meristem organization ( Takada et al, 2001 ), flower development ( Sablowski and Meyerowitz, 1998 ), cell division ( Kim et al, 2006 ), leaf senescence ( Breeze et al, 2011 ), secondary wall formation ( Zhong et al, 2010 ), and biotic and abiotic stress responses ( Olsen et al, 2005 ; Christianson et al, 2010 ; Tran et al, 2010 ; Nakashima et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Drought resistance of tobacco overexpressing the AfNAC1 gene of Amorpha fruticosa Linn.

Liu

Liu³

et al. 2022

Front. Plant Sci.

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Plants are often adversely affected by abiotic stresses such as drought, low temperature, and salinity during growth, and plant NAC-like transcription factors are involved in regulating growth and developmental processes in response to stresses such as drought and salinity. In this study, to investigate the function of AfNAC1, a co-expression network of AfNAC1 genes was constructed using gene expression data from the Chinese legume deciduous shrub, Amorpha fruticosa Linn. A 576 bp NAC transcription factor (AfNAC1 gene, MN180266) encoding 191 amino acids was isolated from Amorpha fruticosa seedlings by RT-PCR. qRT-PCR showed that the AfNAC1 gene was expressed in the roots, stems, leaves, and flowers of Amorpha fruticosa. However, drought stress significantly increased root expression, and the AfNAC1 protein was localized in the nucleus by green fluorescence detection. This study analyzed the drought resistance of overexpressing tobacco in depth. Under natural drought stress, the chlorophyll and antioxidant enzyme activities of overexpressing plants were significantly higher than those of wild-type (WT) plants, but the MDA content was lower than that of WT; after rehydration the Fv/Fm values of AfNAC1-overexpressing tobacco recovered faster than those of wild-type tobacco and rapidly reached the control levels; AfNAC1 may be involved in the regulation of the photosystem and indirectly in the regulation of the plant in response to drought stress.

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“…Although studies on the drought resistance of A. fruticosa WRKY TFs are limited, transcriptome sequencing analysis of A. fruticosa has shown that the AfWRKY20 gene is upregulated in response to drought-induced expression [44]. In this study, the AfWRKY20 gene was cloned from the transcriptome sequencing of A. fruticosa under drought stress using RT-PCR technology.…”

Section: Introductionmentioning

confidence: 99%

Functional Study of Amorpha fruticosa WRKY20 Gene in Response to Drought Stress

Li,

Gu,

Huang

et al. 2023

IJMS

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The WRKY gene family in plants regulates the plant’s response to drought through regulatory networks and hormone signaling. AfWRKY20 (MT859405) was cloned from Amorpha fruticosa (A. fruticosa) seedlings using RT-PCR. The binding properties of the AfWRKY20 protein and the W-box (a DNA cis-acting element) were verified both in vivo and in vitro using EMSA and Dual-Luciferase activity assays. RT-qPCR detected that the total expression level of AfWRKY20 in leaves and roots was 22 times higher in the 30% PEG6000 simulated drought treatment compared to the untreated group. Under the simulated drought stress treatments of sorbitol and abscisic acid (ABA), the transgenic tobacco with the AfWRKY20 gene showed enhanced drought resistance at the germination stage, with significantly increased germination rate, green leaf rate, fresh weight, and root length compared to the wild-type (WT) tobacco. In addition, the superoxide dismutase (SOD) activity, chlorophyll content, and Fv/Fm ratio of AfWRKY20 transgenic tobacco were significantly higher than those of the WT tobacco under natural drought stress, while the malondialdehyde (MDA) content and 3,3′-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) staining levels were lower. The expression levels of oxidation kinase genes (NbSOD, NbPOD, and NbCAT) in transgenic tobacco under drought stress were significantly higher than those in WT tobacco. This enhancement in gene expression improved the ability of transgenic tobacco to detoxify reactive oxygen species (ROS). The survival rate of transgenic tobacco after natural drought rehydration was four times higher than that of WT tobacco. In summary, this study revealed the regulatory mechanism of AfWRKY20 in response to drought stress-induced ABA signaling, particularly in relation to ROS. This finding provides a theoretical basis for understanding the pathways of WRKY20 involved in drought stress, and offers genetic resources for molecular plant breeding aimed at enhancing drought resistance.

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De novo assembly ofAmorpha fruticosaL. transcriptome in response to drought stress provides insight into the tolerance mechanisms

Cited by 7 publications

References 52 publications

Population Genomics Reveals Gene Flow and Adaptive Signature in Invasive Weed Mikania micrantha

Population Genomics Reveals Gene Flow and Adaptive Signature in Invasive Weed Mikania micrantha

Drought resistance of tobacco overexpressing the AfNAC1 gene of Amorpha fruticosa Linn.

Functional Study of Amorpha fruticosa WRKY20 Gene in Response to Drought Stress

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