De novo biosynthesis of 2′-fucosyllactose in a metabolically engineered Escherichia coli using a novel ɑ1,2-fucosyltransferase from Azospirillum lipoferum

Chen, Yihan; Zhu, Yingying; Wang, Hao; Chen, Roulin; Liu, Yuanlin; Zhang, Wenli; Mu, Wanmeng

doi:10.1016/j.biortech.2023.128818

Cited by 25 publications

(30 citation statements)

References 40 publications

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“…Screening of specific glycosyltransferases is an important research topic to solve these problems. For example, a novel α1,2-FucT, A. lipoferum SAMT, did not produce the byproducts DFL and 3-FL while ensuring the high productivity of 2′-FL . In addition, protein engineering and molecular modification are expected to improve the substrate specificity and regioselectivity of HMO-producing glycosyltransferases; however, these related studies are still limited.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…57 Therefore, the research on α1,2-FucTs and microbial synthesis of 2′-FL is particularly important in the current studies on HMOs biosynthesis. At least 15 bacterial α1,2-FucTs have been identified and used in the biosynthesis of HMOs, which were from Azospirillum lipoferum (named SAMT), 58 Bacillus cereus VD107 (FutBc), 59 Bacteroides f ragilis NCTC 9343 (WcfB), 60 E. coli O86 (WbnK), 61 E. coli O126 (WbgL), 62 E. coli O86:B7 (WbwK), 63 E. coli O127 (WbiQ), E. coli O128 (WbsJ), Enterocloster bolteae (FutCbo), 64 Helicobacter mustelae (FutL), 65 Helicobacter pylori 26695 (FutC), 66 Helicobacter pylori UA802 (FucT2), 67 Helicobacter sp. 11S02629−2 (BKHT), 68 Thermosynechococcus elongatus (Te2FT), 69 and Thermosynechococcus sp.…”

Section: Gdp-fuc-dependent Fuctsmentioning

confidence: 99%

“…Using HPAEC−PAD detection, two α1,2-FucTs, A. lipoferum SAMT 58 and Helicobacter sp. BKHT, 68 were screened with highly efficient in vivo 2′-FL synthesis ability and without α1,3-FucT activity, and the constructed metabolically engineered E. coli strains produced 112.56 and 94.7 g/L by fed-batch cultivation, respectively.…”

Section: Gdp-fuc-dependent Fuctsmentioning

confidence: 99%

“…For example, a novel α1,2-FucT, A. lipoferum SAMT, did not produce the byproducts DFL and 3-FL while ensuring the high productivity of 2′-FL. 58 In addition, protein engineering and molecular modification are expected to improve the substrate specificity and regioselectivity of HMO-producing glycosyltransferases; however, these related studies are still limited. Protein engineering is also an important approach to enhancing specific activity.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…It is possible that previous studies might ignore the accurate detection of byproducts, so there actually might be more α1,2-FucTs possessing α1,3-FucT activity in the reported α1,2-FucTs. Using HPAEC–PAD detection, two α1,2-FucTs, A. lipoferum SAMT and Helicobacter sp. BKHT, were screened with highly efficient in vivo 2′-FL synthesis ability and without α1,3-FucT activity, and the constructed metabolically engineered E. coli strains produced 112.56 and 94.7 g/L by fed-batch cultivation, respectively.…”

Section: Gdp-fuc-dependent Fuctsmentioning

confidence: 99%

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An Overview of Sugar Nucleotide-Dependent Glycosyltransferases for Human Milk Oligosaccharide Synthesis

Zhao

Zhu

Wang³

et al. 2023

J. Agric. Food Chem.

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Human milk oligosaccharides (HMOs) have received increasing attention because of their special effects on infant health and commercial value as the new generation of core components in infant formula. Currently, large-scale production of HMOs is generally based on microbial synthesis using metabolically engineered cell factories. Introduction of the specific glycosyltransferases is essential for the construction of HMO-producing engineered strains in which the HMO-producing glycosyltransferases are generally sugar nucleotide-dependent. Four types of glycosyltransferases have been used for typical glycosylation reactions to synthesize HMOs. Soluble expression, substrate specificity, and regioselectivity are common concerns of these glycosyltransferases in practical applications. Screening of specific glycosyltransferases is an important research topic to solve these problems. Molecular modification has also been performed to enhance the catalytic activity of various HMO-producing glycosyltransferases and to improve the substrate specificity and regioselectivity. In this article, various sugar nucleotide-dependent glycosyltransferases for HMO synthesis were overviewed, common concerns of these glycosyltransferases were described, and the future perspectives of glycosyltransferase-related studies were provided.

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Section: Future Perspectivesmentioning

confidence: 99%

Section: Gdp-fuc-dependent Fuctsmentioning

confidence: 99%

Section: Gdp-fuc-dependent Fuctsmentioning

confidence: 99%

Section: Future Perspectivesmentioning

confidence: 99%

Section: Gdp-fuc-dependent Fuctsmentioning

confidence: 99%

See 3 more Smart Citations

An Overview of Sugar Nucleotide-Dependent Glycosyltransferases for Human Milk Oligosaccharide Synthesis

Zhao

Zhu

Wang³

et al. 2023

J. Agric. Food Chem.

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De novo biosynthesis of 2ʹ‐fucosyllactose by bioengineered Corynebacterium glutamicum

Lee,

Jo,

Lee

et al. 2023

Biotechnology Journal

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Abstract2ʹ‐Fucosyllactose (2ʹ‐FL) which is well‐known human milk oligosaccharide was biotechnologically synthesized using engineered Corynebacterium glutamicum, a GRAS microbial workhorse. By construction of the complete de novo pathway for GDP‐L‐fucose supply and heterologous expression of Escherichia coli lactose permease and Helicobacter pylori α‐1,2‐fucosyltransferase, bioengineered C. glutamicum BCGW_TL successfully biosynthesized 0.25 g/L 2ʹ‐FL from glucose. The additional genetic perturbations including the expression of a putative 2ʹ‐FL exporter and disruption of the chromosomal pfkA gene allowed C. glutamicum BCGW_cTTLEΔP to produce 2.5 g/L 2ʹ‐FL batchwise. Finally, optimized fed‐batch cultivation of the BCGW_cTTLEΔP using glucose, fructose, and lactose resulted in 21.5 g/L 2ʹ‐FL production with a productivity of 0.12 g/L•h, which were more than 3.3 times higher value relative to the batch culture of the BCGW_TL. Conclusively, it would be a groundwork to adopt C. glutamicum for biotechnological production of other food additives including human milk oligosaccharides.This article is protected by copyright. All rights reserved

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Construction and application of 3-fucosyllactose whole-cell biosensor for high-throughput screening of overproducers

Li,

Liu,

et al. 2024

Bioresource Technology

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De novo biosynthesis of 2′-fucosyllactose in a metabolically engineered Escherichia coli using a novel ɑ1,2-fucosyltransferase from Azospirillum lipoferum

Cited by 25 publications

References 40 publications

An Overview of Sugar Nucleotide-Dependent Glycosyltransferases for Human Milk Oligosaccharide Synthesis

An Overview of Sugar Nucleotide-Dependent Glycosyltransferases for Human Milk Oligosaccharide Synthesis

De novo biosynthesis of 2ʹ‐fucosyllactose by bioengineered Corynebacterium glutamicum

Construction and application of 3-fucosyllactose whole-cell biosensor for high-throughput screening of overproducers

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