De novo discovery of neuropeptides in the genomes of parasitic flatworms using a novel comparative approach

Koziol, Uriel; Koziol, Miguel; Preza, Matías; Costábile, Alicia; Brehm, Klaus; Castillo, Estela

doi:10.1016/j.ijpara.2016.05.007

Cited by 53 publications

(72 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results also give new information about expression of neuropeptides in schistosomes. Based on the discovery of novel neuropeptide precursors (NPPs) in flatworms (Koziol et al 2016), we found that most of SmNPPs were preferentially-expressed in sM-sF-bM (Fig. 8C), a pattern identified recently in the adult stage (Lu et al 2016).…”

Section: Discussionmentioning

confidence: 82%

Meta-analysis of RNA-seq studies reveals genes responsible for life stage-dominant functions in Schistosoma mansoni

Berriman

2018

Preprint

View full text Add to dashboard Cite

Background: Since the genome of the parasitic flatworm Schistosoma mansoni was sequenced in 2009, various RNA-seq studies have been conducted to investigate differential gene expression between certain life stages. Based on these studies, the overview of gene expression in all life stages can improve our understanding of S. mansoni genome biology. Methods: publicly available RNA-seq data covering all life stages and gonads were mapped to the latest S. mansoni genome. Read counts were normalised across all samples and differential expression analysis was preformed using the generalized linear model (GLM) approach. Results: we revealed for the first time the dissimilarities among all life stages. Genes that are abundantly-expressed in all life stages, as well as those preferentiallyexpressed in certain stage(s), were determined. The latter reveals genes responsible for stagedominant functions of the parasite, which can be a guidance for the investigation and annotation of gene functions. In addition, distinct differential expression patterns were observed between adjacent life stages, which not only correlate well with original individual studies, but also provide additional information on changes in gene expression during parasite transitions. Furthermore, thirteen novel housekeeping genes across all life stages were identified, which is valuable for quantitative studies (e.g., qPCR). Conclusions: the metaanalysis provides valuable information on the expression and potential functions of S. mansoni genes across all life stages, and can facilitate basic as well as applied research for the community.

show abstract

Section: Discussionmentioning

confidence: 82%

Meta-analysis of RNA-seq studies reveals genes responsible for life stage-dominant functions in Schistosoma mansoni

Berriman

2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Our phylogeny (Figure 2A) suggests that fwRhods are more similar to peptide than amine receptors. If their heterologous expression can be achieved, one approach to characterisation would be to screen them with the growing canon of peptide ligands from flatworms [36–38], as well as from other genera, in a receptor activation assay. Subsequent localisation of their spatial expression patterns would provide additional data that would inform function.…”

Section: Resultsmentioning

confidence: 99%

Profiling G protein-coupled receptors of Fasciola hepatica identifies orphan rhodopsins unique to phylum Platyhelminthes

McVeigh

McCammick

McCusker

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

“…They might hold important, yet hidden, functional aspects that would allow a complete understanding of the interaction between infective plerocercoids and their fish host (Koziol et al . ).…”

Section: Detecting Distinct Developmental Stages Within the Same Hostmentioning

confidence: 97%

“…These sequences were thus labelled as infective-specific and coexpressed with genes involved in environmental sensing and interaction (Table S1, Supporting information). They might hold important, yet hidden, functional aspects that would allow a complete understanding of the interaction between infective plerocercoids and their fish host (Koziol et al 2016).…”

Section: Detecting Distinct Developmental Stages Within the Same Hostmentioning

confidence: 99%

Major host transitions are modulated through transcriptome‐wide reprogramming events in Schistocephalus solidus, a threespine stickleback parasite

et al. 2017

View full text Add to dashboard Cite

Parasites with complex life cycles have developed numerous phenotypic strategies, closely associated with developmental events, to enable the exploitation of different ecological niches and facilitate transmission between hosts. How these environmental shifts are regulated from a metabolic and physiological standpoint, however, still remain to be fully elucidated. We examined the transcriptomic response of Schistocephalus solidus, a trophically transmitted parasite with a complex life cycle, over the course of its development in an intermediate host, the threespine stickleback, and the final avian host. Results from our differential gene expression analysis show major reprogramming events among developmental stages. The final host stage is characterized by a strong activation of reproductive pathways and redox homoeostasis. The attainment of infectivity in the fish intermediate host—which precedes sexual maturation in the final host and is associated with host behaviour changes—is marked by transcription of genes involved in neural pathways and sensory perception. Our results suggest that un‐annotated and S. solidus‐specific genes could play a determinant role in host–parasite molecular interactions required to complete the parasite's life cycle. Our results permit future comparative analyses to help disentangle species‐specific patterns of infection from conserved mechanisms, ultimately leading to a better understanding of the molecular control and evolution of complex life cycles.

show abstract

De novo discovery of neuropeptides in the genomes of parasitic flatworms using a novel comparative approach

Cited by 53 publications

References 70 publications

Meta-analysis of RNA-seq studies reveals genes responsible for life stage-dominant functions in Schistosoma mansoni

Meta-analysis of RNA-seq studies reveals genes responsible for life stage-dominant functions in Schistosoma mansoni

Profiling G protein-coupled receptors of Fasciola hepatica identifies orphan rhodopsins unique to phylum Platyhelminthes

Major host transitions are modulated through transcriptome‐wide reprogramming events in Schistocephalus solidus, a threespine stickleback parasite

Contact Info

Product

Resources

About