De novo generation of macrophage from placenta-derived hemogenic endothelium

Liang, Guixian; Zhou, Chunyu; Jiang, Xiangxiang; Zhang, Yifan; Huang, Biao; Gao, Song; Kang, Zhixin; Ma, Dongyuan; Wang, Fengchao; Göttgens, Berthold; Wang, Hongmei; Han, Jing‐Dong J.; Liu, Feng

doi:10.1016/j.devcel.2021.06.005

Cited by 29 publications

(26 citation statements)

References 74 publications

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“…Of relevance here are differences between murine and human placental macrophages. Murine placental macrophages have been proposed to be analogous to human HBC, hence they have been termed HBC-like cells ( 77 ). However, murine placental macrophages that have been characterized thus far are not like human HBC in terms of ontogeny and localization.…”

Section: Challenges and Experimental Models For The Future Study Of Placental Macrophagesmentioning

confidence: 99%

The Ontogeny and Function of Placental Macrophages

et al. 2021

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The placenta is a fetal-derived organ whose function is crucial for both maternal and fetal health. The human placenta contains a population of fetal macrophages termed Hofbauer cells. These macrophages play diverse roles, aiding in placental development, function and defence. The outer layer of the human placenta is formed by syncytiotrophoblast cells, that fuse to form the syncytium. Adhered to the syncytium at sites of damage, on the maternal side of the placenta, is a population of macrophages termed placenta associated maternal macrophages (PAMM1a). Here we discuss recent developments that have led to renewed insight into our understanding of the ontogeny, phenotype and function of placental macrophages. Finally, we discuss how the application of new technologies within placental research are helping us to further understand these cells.

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Section: Challenges and Experimental Models For The Future Study Of Placental Macrophagesmentioning

confidence: 99%

The Ontogeny and Function of Placental Macrophages

et al. 2021

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“…However, EMPs can also be produced by venous HE cells in the plexus of the YS ( 55 , 56 ) and from HE cells in the heart that have not yet acquired an arterial-venous specification ( 57 ). Macrophages can also directly derive from HE cells via EHT in the placenta ( 58 ). The mere presence of HE cells does not guarantee that an EHT event occurs, indicating that the EHT process is differentially steered by signals from the niche, depending on their precise anatomical location and surroundings.…”

Section: Hematopoietic Production Occurs In Various Highly Vascularized Tissues During Developmentmentioning

confidence: 99%

Recent Advances in Developmental Hematopoiesis: Diving Deeper With New Technologies

2021

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The journey of a hematopoietic stem cell (HSC) involves the passage through successive anatomical sites where HSCs are in direct contact with their surrounding microenvironment, also known as niche. These spatial and temporal cellular interactions throughout development are required for the acquisition of stem cell properties, and for maintaining the HSC pool through balancing self-renewal, quiescence and lineage commitment. Understanding the context and consequences of these interactions will be imperative for our understanding of HSC biology and will lead to the improvement of in vitro production of HSCs for clinical purposes. The aorta-gonad-mesonephros (AGM) region is in this light of particular interest since this is the cradle of HSC emergence during the embryonic development of all vertebrate species. In this review, we will focus on the developmental origin of HSCs and will discuss the novel technological approaches and recent progress made to identify the cellular composition of the HSC supportive niche and the underlying molecular events occurring in the AGM region.

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“…This protocol describes the steps to sort out HE cells from mouse placenta ( Liang et al., 2021 ). It was primarily optimized using mid-gestational placenta, which was dissected from embryonic day (E)11.0 pregnant mice.…”

Section: Before You Beginmentioning

confidence: 99%

“…We also describe techniques for dissection of placental tissues and for the optimization of tissue digestion and antibody conjugation conditions to identify HE cells via fluorescence-activated cell sorting. For details on the usage and application of this protocol, please refer to Liang et al. (2021) .…”

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Protocols for isolation and characterization of mouse placental hemogenic endothelial cells

et al. 2021

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Summary The murine mid-gestational placenta has been identified as a hematopoietic site during embryonic development. Here, we describe a protocol for isolation and characterization of the hemogenic endothelial (HE) cells from mouse placenta. We also describe techniques for dissection of placental tissues and for the optimization of tissue digestion and antibody conjugation conditions to identify HE cells via fluorescence-activated cell sorting. For details on the usage and application of this protocol, please refer to Liang et al. (2021) .

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De novo generation of macrophage from placenta-derived hemogenic endothelium

Cited by 29 publications

References 74 publications

The Ontogeny and Function of Placental Macrophages

The Ontogeny and Function of Placental Macrophages

Recent Advances in Developmental Hematopoiesis: Diving Deeper With New Technologies

Protocols for isolation and characterization of mouse placental hemogenic endothelial cells

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