2012
DOI: 10.1016/j.ygeno.2012.02.002
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De novo intestine-specific transcriptome of the brown planthopper Nilaparvata lugens revealed potential functions in digestion, detoxification and immune response

Abstract: The brown planthopper (Nilaparvata lugens, BPH) is the most serious rice plant pests in Asia. In this study, we performed transcriptome-wide analysis on BPH intestine. We obtained more than 26 million sequencing reads that were then assembled into 53,553 unigenes with a mean size of 388 bp. Based on similarity search with the nucleotide sequences available at NCBI, BPH intestine-specific transcriptome analysis identified 21,405 sequences. Assembled sequences were annotated with gene description, gene ontology … Show more

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Cited by 75 publications
(67 citation statements)
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“…JN662398) was analyzed using the following primers: 5¢- CGCTACTACCGATTGAA- 3¢ (sense) and 5¢- GGAAACCTTGTTACGACTT- 3¢ (antisense). In our previous studies, the utility of the N. lugens 18S rRNA gene was validated for its stable expression in N. lugens tissues, developmental stages and immune-induced individuals182021. In the present study, the results were standardized to the expression level of N. lugens 18S rRNA .…”
Section: Methodsmentioning
confidence: 97%
See 1 more Smart Citation
“…JN662398) was analyzed using the following primers: 5¢- CGCTACTACCGATTGAA- 3¢ (sense) and 5¢- GGAAACCTTGTTACGACTT- 3¢ (antisense). In our previous studies, the utility of the N. lugens 18S rRNA gene was validated for its stable expression in N. lugens tissues, developmental stages and immune-induced individuals182021. In the present study, the results were standardized to the expression level of N. lugens 18S rRNA .…”
Section: Methodsmentioning
confidence: 97%
“…Apoptotic signals trigger initiator caspases, which function to activate downstream effector caspases that lack long prodomains and the ability to self-activate, and lead to programmed cell death1417. We identified five caspase homolog genes through searching the N. lugens genome and transcriptome datasets1819202122. Three genes are highly homologous to each other and share significant sequence similarities with insect caspase-1 genes112324252627.…”
Section: Introductionmentioning
confidence: 99%
“…e l s e v i e r . c o m / l o c a t e / g e n r e p 2011; Hua et al, 2011;Bao et al, 2012;Zheng et al, 2013;Gao et al, 2013). At present, the transcriptome sequencing technology has gradually replaced the gene chip technology and becomes the mainstream method for studying gene expression profiling at the level of whole genome.…”
Section: Contents Lists Available At Sciencedirectmentioning
confidence: 99%
“…The purified colony was used for genomic DNA sequencing [19] and the experiments performed in this study. The insects were reared at 26 ± 0.5°C with 50 ± 5% humidity on rice seedlings (Oryza sativa strain TN1) under a 16:8 hour light:dark photoperiod as previously described [20][21][22]. Technical imidacloprid, triazophos and deltamethrin were supplied by Xinnong Chemical Industrial Group Co. Ltd. (Taizhou, Zhejiang, China).…”
Section: Insects and Insecticidesmentioning
confidence: 99%
“…The N. lugens genome is the first characterized genome of a monophagous sap-sucking arthropod herbivore, and the availability of its genome sequence allows the global analysis of P450 genes, which will yield insights into the resistance mechanisms against a variety of insecticides in this hemimetabolous insect species. Previously, we performed transcriptome sequencing using next-generation highthroughput Illumina technology, which generated detailed transcript information about the developmental stages, wing dimorphism, sex differences, immune responses and tissue specificity in N. lugens [20][21][22]. Here, a thorough search of the N. lugens genome sequence coupled with analyses of the available transcriptome datasets provided comprehensive information about the P450 genes, which can be classified into at least four P450 subgroups based on sequence homology and subcellular location.…”
Section: Introductionmentioning
confidence: 99%