De novo synthesis of glutathione in human fibroblasts during in vitro ageing and in some metabolic diseases as measured by a flow cytometric method

Poot, Martin; Verkerk, Anton; Koster, J.F.; Jongkind, J. F.

doi:10.1016/0304-4165(86)90300-4

Cited by 57 publications

(22 citation statements)

References 21 publications

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“…Intracellular GSH level was determined using a thiol-staining reagent, mBBr, according to a previous method with slight modification (Poot et al, 1986). RAW264.7 cells were seeded at a concentration of 4.0 × 10 5 cells/mL and following confluence, were treated with the chitosan-phloroglucinol conjugate for 1 h. The cells were then labeled with 40 µM mBBr for 30 min, and then mBBr-GSH fluorescence intensity was measured at an excitation wavelength of 360 nm and an emission wavelength of 465 nm using a spectrofluorometer (Spectra Max).…”

Section: H Nmr Analysis Wasmentioning

confidence: 99%

Antioxidant and Antibacterial Activities of Chitosan-Phloroglucinol Conjugate

Lee¹,

Cho²,

2013

Fisheries and aquatic sciences

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In an effort to develop biopolymer-based antioxidant and antibacterial materials, a chitosan-phloroglucinol conjugate was prepared and cellular antioxidant activity and minimum inhibitory concentration against foodborne pathogens and methicillin-resistant Staphylococcus aureus (MRSA) evaluated. The chitosan-phloroglucinol conjugate showed higher antioxidant activities than the unmodified chitosan (P < 0.05). The chitosan-phloroglucinol conjugate showed 62.29% reactive oxygen species scavenging activity, 56.11% lipid peroxidation inhibition activity, and 2.21-fold increase of glutathione expression in mouse macrophage cells. Additionally, the chitosan-phloroglucinol conjugate exhibited higher antibacterial activities than the unmodified chitosan, and the chitosan-phloroglucinol conjugate showed fourfold higher antibacterial activities against MRSA and clinical isolates and twofold higher activities against foodborne pathogens compared to the unmodified chitosan.

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Section: H Nmr Analysis Wasmentioning

confidence: 99%

Antioxidant and Antibacterial Activities of Chitosan-Phloroglucinol Conjugate

Lee¹,

Cho²,

2013

Fisheries and aquatic sciences

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“…Fluorescence measurements were done with the following dyes as previously described: [31][32][33] MCB and monobromobimane to measure GSH and reduced cellular thiols, respectively; CMX to measure mitochondrial membrane potential; MTG to measure mitochondrial mass; NAO to measure cardiolipin content; and dichloromethyl-x-rosamine (H2-CMXROS), a mitochondrial selective dye that becomes fluorescent when oxidized, to measure mitochondrial ROS. UV excited blue autofluorescence was used to measure reduced NAD, designated NADPH to represent contributions by both NADH and NADPH.…”

Section: Flow Cytometric Detection Of Cellular Redox State and Mitochmentioning

confidence: 99%

Disruption of Redox Homeostasis in Tumor Necrosis Factor-Induced Apoptosis in a Murine Hepatocyte Cell Line

Pierce¹,

Campbell²,

Stephenson³

et al. 2000

The American Journal of Pathology

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114

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Tumor necrosis factor (TNF) is a mediator of the acute phase response in the liver and can initiate proliferation and cause cell death in hepatocytes. We investigated the mechanisms by which TNF causes apoptosis in hepatocytes focusing on the role of oxidative stress, antioxidant defenses, and mitochondrial damage. The studies were conducted in cultured AML12 cells, a line of differentiated murine hepatocytes. As is the case for hepatocytes in vivo, AML12 cells were not sensitive to cell death by TNF alone, but died by apoptosis when exposed to TNF and a small dose of actinomycin D (Act D). Morphological signs of apoptosis were not detected until 6 hours after the treatment and by 18 hours ϳ50% of the cells had died. Exposure of the cells to TNF؉Act D did not block NFB nuclear translocation, DNA binding, or its overall transactivation capacity. Induction of apoptosis was characterized by oxidative stress indicated by the loss of NAD(P)H and glutathione followed by mitochondrial damage that included loss of mitochondrial membrane potential, inner membrane structural damage, and mitochondrial condensation. These changes coincided with cytochrome C release and the activation of caspases-8, -9, and -3. TNF-induced apoptosis was dependent on glutathione levels.

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“…The ESR is an effective approach to examine unstable free radicals and only requires simple sample preparation, short experimental time, and is highly sensitive [41]. Scavenging activities were calculated based on relative peak height in the ESR spectra of treatment groups compared with the control group.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant activity of the halophyte Limonium tetragonum and its major active components

Lee

Kong

Jung

et al. 2011

Biotechnol Bioproc E

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In this study, the antioxidant potentials of crude extracts and solvent-partitioned fractions of Limonium tetragonum were assessed by measuring their ability to scavenge intracellular reactive oxygen species (ROS) generated in HT-1080 cells. Following activity-oriented separation, four flavonol glycosides were isolated as active principles and their chemical structures were determined by 2 D NMR and by comparison with reported spectral data. The isolated compounds (1-4) were evaluated for their antioxidant capacity using three different activity tests; degree of occurrence of intracellular ROS, lipid peroxidation in HT-1080 cells and the extent of oxidative damage of genomic DNA purified from HT-1080 cells. All compounds exhibited significantly inhibited the generation of intracellular ROS and lipid peroxidation in HT-1080 cells, and significantly inhibited DNA oxidation. In addition, direct free radical scavenging effects of these compounds were investigated using the electron spin resonance (ESR) spin-trap technique.

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De novo synthesis of glutathione in human fibroblasts during in vitro ageing and in some metabolic diseases as measured by a flow cytometric method

Cited by 57 publications

References 21 publications

Antioxidant and Antibacterial Activities of Chitosan-Phloroglucinol Conjugate

Antioxidant and Antibacterial Activities of Chitosan-Phloroglucinol Conjugate

Disruption of Redox Homeostasis in Tumor Necrosis Factor-Induced Apoptosis in a Murine Hepatocyte Cell Line

Antioxidant activity of the halophyte Limonium tetragonum and its major active components

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