De novo transcriptome analysis of tobacco seedlings and identification of the early response gene network under low-potassium stress

Li, L. Q.; Li, J.; Chen, Y.; Lu, Yanli; Lu, Lin

doi:10.4238/gmr.15038599

Cited by 8 publications

(7 citation statements)

References 37 publications

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“…It was shown that although changes in K + supply (either short- or long-term) affected the expression of a large number of membrane transporter genes, but surprisingly only a few genes belonged to K + transporters that have been reported. Similar results were reported for Arabidopsis [ 31 ], wheat and rice [ 66 ], and model legume, M. truncatula [ 63 ]. Also, only three GhHAK5 genes and one GhKUP3 gene were induced by low K + in the roots of both grafts in this study ( Figure 6 a).…”

Section: Discussionsupporting

confidence: 84%

Transcriptome Analysis Unravels Key Factors Involved in Response to Potassium Deficiency and Feedback Regulation of K+ Uptake in Cotton Roots

Yang

et al. 2021

IJMS

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To properly understand cotton responses to potassium (K+) deficiency and how its shoot feedback regulates K+ uptake and root growth, we analyzed the changes in root transcriptome induced by low K+ (0.03 mM K+, lasting three days) in self-grafts of a K+ inefficient cotton variety (CCRI41/CCRI41, scion/rootstock) and its reciprocal grafts with a K+ efficient variety (SCRC22/CCRI41). Compared with CCRI41/CCRI41, the SCRC22 scion enhanced the K+ uptake and root growth of CCRI41 rootstock. A total of 1968 and 2539 differently expressed genes (DEGs) were identified in the roots of CCRI41/CCRI41 and SCRC22/CCRI41 in response to K+ deficiency, respectively. The overlapped and similarly (both up- or both down-) regulated DEGs in the two grafts were considered the basic response to K+ deficiency in cotton roots, whereas the DEGs only found in SCRC22/CCRI41 (1954) and those oppositely (one up- and the other down-) regulated in the two grafts might be the key factors involved in the feedback regulation of K+ uptake and root growth. The expression level of four putative K+ transporter genes (three GhHAK5 and one GhKUP3) increased in both grafts under low K+, which could enable plants to cope with K+ deficiency. In addition, two ethylene response factors (ERFs), GhERF15 and GhESE3, both down-regulated in the roots of CCRI41/CCRI41 and SCRC22/CCRI41, may negatively regulate K+ uptake in cotton roots due to higher net K+ uptake rate in their virus-induced gene silencing (VIGS) plants. In terms of feedback regulation of K+ uptake and root growth, several up-regulated DEGs related to Ca2+ binding and CIPK (CBL-interacting protein kinases), one up-regulated GhKUP3 and several up-regulated GhNRT2.1s probably play important roles. In conclusion, these results provide a deeper insight into the molecular mechanisms involved in basic response to low K+ stress in cotton roots and feedback regulation of K+ uptake, and present several low K+ tolerance-associated genes that need to be further identified and characterized.

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Section: Discussionsupporting

confidence: 84%

Transcriptome Analysis Unravels Key Factors Involved in Response to Potassium Deficiency and Feedback Regulation of K+ Uptake in Cotton Roots

Yang

et al. 2021

IJMS

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“…CPK13 specifically inhibits guard cell-expressed KAT2 and KAT1 shaker K + channels [ 85 ]. The results of a recent study [ 86 ] showed that two CDPKs are up-regulated in tobacco seedlings under low-K + stress, whereas OsCPK9 and OsCCaMK1 are down-regulated in rice root responses to K + deficiency [ 87 ].…”

Section: Molecular Mechanisms Of Calcium Signaling Involved In Plamentioning

confidence: 99%

Plant Calcium Signaling in Response to Potassium Deficiency

Wang

Líu

Zhu

et al. 2018

IJMS

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Potassium (K+) is an essential macronutrient of living cells and is the most abundant cation in the cytosol. K+ plays a role in several physiological processes that support plant growth and development. However, soil K+ availability is very low and variable, which leads to severe reductions in plant growth and yield. Various K+ shortage-activated signaling cascades exist. Among these, calcium signaling is the most important signaling system within plant cells. This review is focused on the possible roles of calcium signaling in plant responses to low-K+ stress. In plants, intracellular calcium levels are first altered in response to K+ deficiency, resulting in calcium signatures that exhibit temporal and spatial features. In addition, calcium channels located within the root epidermis and root hair zone can then be activated by hyperpolarization of plasma membrane (PM) in response to low-K+ stress. Afterward, calcium sensors, including calmodulin (CaM), CaM-like protein (CML), calcium-dependent protein kinase (CDPK), and calcineurin B-like protein (CBL), can act in the sensing of K+ deprivation. In particular, the important components regarding CBL/CBL-interacting protein kinase (CBL/CIPK) complexes-involved in plant responses to K+ deficiency are also discussed.

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“…In low potassium treated pear fruit, there multiple cytochrome P450 members were detected [34]. In low potassium treated tobacco, 3 cytochrome P450 members were also up-regulated [37]. The DEGs belong to cytochrome P450 family were also detected from potassium deficiency tomato [38], low-N polar [39], rice [40], sorghum [41] and wild barley [42].…”

Section: Discussionmentioning

confidence: 98%

Transcriptome Changes Induced by Different Potassium Levels in Banana Roots

Lin

et al. 2019

Plants

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Potassium plays an important role in enhancing plant resistance to biological and abiotic stresses and improving fruit quality. To study the effect of potassium nutrient levels on banana root growth and its regulation mechanism, four potassium concentrations were designed to treat banana roots from no potassium to high potassium. The results indicated that K2 (3 mmol/L K 2 SO 4 ) treatment was a relatively normal potassium concentration for the growth of banana root, and too high or too low potassium concentration was not conducive to the growth of banana root. By comparing the transcriptome data in each treatment in pairs, 4454 differentially expressed genes were obtained. There were obvious differences in gene function enrichment in root systems treated with different concentrations of potassium. Six significant expression profiles (profile 0, 1, 2, 7, 9 and 13) were identified by STEM analysis. The hub genes were FKF1, HsP70-1, NRT1/PTR5, CRY1, and ZIP11 in the profile 0; CYP51 in profile 1; SOS1 in profile 7; THA, LKR/SDH, MCC, C4H, CHI, F3 H, 2 PR1s, BSP, TLP, ICS, RO, chitinase and peroxidase in profile 9. Our results provide a comprehensive and systematic analysis of the gene regulation network in banana roots under different potassium stress.Plants 2020, 9, 11 2 of 24 area [18]. The lack of potassium had a significant effect on the growth of rapeseed, and the lack of potassium significantly inhibited the growth of taproots and lateral roots [19]. Potassium deficiency of tobacco decreased root growth and mainly affected the formation and elongation of primary lateral roots [20]. Low potassium not only significantly reduced the dry weight of cabbage root, but also reduced its leaf area [21]. Therefore, the morphological structure of root system was greatly affected by potassium stress. It is significant to study the response mechanism of root system under potassium stress. There has been extensive research on root structural changes in response to stress of nutrients such as phosphate and nitrate and the signaling pathways that mediate these changes [22], and practical breakthroughs have been made. However, the mechanism of root structural change and response to potassium stress is still unclear.With the purpose to explore the molecular mechanism of plant root response to potassium pressure, there are more and more reports on transcriptome, metabolome and proteomics of potassium stress (especially low potassium stress) in recent years. Transcriptome responses to K starvation in Arabidopsis thaliana [16], rice [4], soybean [23] and wild barley [24] indicated that genes related to ion transport, metabolism, signal transduction and protein phosphorylation significantly changed. Transcriptional analysis of sugarcane under low potassium stress showed significant differences in the expression of transcription factors, ion transporters, protein kinases and genes related to oxidative stress in the Ca 2+ signal and ethylene signal pathways [25]. The results of transcriptome of rice potassium deficient seedlings show...

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De novo transcriptome analysis of tobacco seedlings and identification of the early response gene network under low-potassium stress

Cited by 8 publications

References 37 publications

Transcriptome Analysis Unravels Key Factors Involved in Response to Potassium Deficiency and Feedback Regulation of K+ Uptake in Cotton Roots

Transcriptome Analysis Unravels Key Factors Involved in Response to Potassium Deficiency and Feedback Regulation of K+ Uptake in Cotton Roots

Plant Calcium Signaling in Response to Potassium Deficiency

Transcriptome Changes Induced by Different Potassium Levels in Banana Roots

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