De novo transcriptomic analysis to reveal insecticide action and detoxification-related genes of the predatory bug, Cyrtorhinus lividipennis

Liu, Siyi; Nelson, David R.; Zhao, Jing; Hua, Hongxia; He, Yueping

doi:10.1016/j.aspen.2017.04.010

Cited by 5 publications

(13 citation statements)

References 41 publications

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“…Therefore, increasing studies on this key biological agent, C. lividipennis, have been published in recent years. For a better understanding, conservation and largescale rearing of this natural enemy, researchers are currently using multiple molecular approaches to investigate the predatory behaviour, pesticide resistance, development and reproduction of C. lividipennis (Ge et al, 2020;Liu et al, 2017;Wang et al, 2018;Zhu et al, 2020). However, low-quality transcriptome data and lack of genomic information limit further research.…”

Section: Introductionmentioning

confidence: 99%

Chromosome‐level genome assembly of the mirid predator Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae), an important natural enemy in the rice ecosystem

Bai

Shi

Zhou

et al. 2021

Molecular Ecology Resources

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Though the genomes of many rice herbivorous pests have recently been well characterized, little is known about the genome of their natural enemies. Here, by using the Illumina and PacBio platforms, we sequenced and assembled the whole genome of the mirid species Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae), which is an economically and ecologically important natural enemy in the rice ecosystem acting as a dominant predator for planthoppers and leafhoppers in the field. Through Hi‐C scaffolding, 1615 scaffolds with a total size of 338.08 Mb were successfully anchored onto 13 chromosomes. The assembled genome size was 345.75 Mb with a final scaffold N50 of 27.58 Mb. Approximately 107.51 Mb of sequences accounting for 31.10% of the genome were identified as repeat elements, and 14,644 protein‐coding genes were annotated. Phylogenetic analysis showed that C. lividipennis clustered with other Hemipteran species and diverged from Apolygus lucorum about 66.7 million years ago. Gene families related to detoxification, environmental adaptation and digestion were analysed comparatively with other Hemipteran species, but no significant expansion or contraction was found in C. lividipennis. We also observed male meiosis in C. lividipennis, which showed a typical post‐reduction of sex chromosomes and a karyotype of 2n = 22 + XY. As the first natural‐enemy genome in the rice ecosystem, the genomic resource of C. lividipennis not only expands our understanding of the multitrophic interactions (host plant–prey–predator), but also provides a genomic basis for better understanding this dominant predator and therefore promotes sustainable rice pest management and food grain production.

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Section: Introductionmentioning

confidence: 99%

Chromosome‐level genome assembly of the mirid predator Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae), an important natural enemy in the rice ecosystem

Bai

Shi

Zhou

et al. 2021

Molecular Ecology Resources

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“…The genetic and molecular responses initiated by different toxicities were revealed in some predatory arthropods, including Propylea japonica, Cyrtorhinus lividipennis, Neoseiulus barkeri, and Pardosa pseudoannulata . Most chemical insecticide molecules and the metabolites derived from their degradation are among the environmental toxicities and arouse various responses in predatory arthropods. , However, genetic information on predatory stink bug species in response to chemical insecticides is lacking.…”

Section: Discussionmentioning

confidence: 99%

Detrimental Impact of λ-Cyhalothrin on the Biocontrol Efficacy of Eocanthecona furcellata by Affecting Global Transcriptome and Predatory Behavior

Yao

Quan

et al. 2022

J. Agric. Food Chem.

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Whether and how insecticide exposure will affect the biological control efficacy of predatory arthropods is critical in insecticide toxicology research but largely unexplored. In the current study, reduced biocontrol efficacy was observed in a predatory stink bugEocanthecona furcellataafter insecticide application in the field. Thus, we constructed a comparative transcriptome analysis and identified a total of 4364 upregulated and 1043 down regulated differentially expressed genes following the sublethal exposure of λ-cyhalothrin. The reduced juvenile hormone (JH) titer and increased trehalose content were observed. The predation capacity and theoretical maximum predation of predators were decreased by 31.08 and 48.90% in response to λ-cyhalothrin, respectively. Furthermore, JH supplementation after λ-cyhalothrin treatment could significantly stimulate trehalase and detoxification enzyme activities, as well as restore the predatory ability of E. furcellata. Our results help to understand the toxicological mechanism of predatory stink bug species in responding to insecticides, benefit predators' ecological services, and optimize the insecticide selection.

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“…Each 20 μl reaction mixture contained 10 μl SYBR Green Realtime PCR Master Mix (Toyobo, Osaka, Japan), 1 μl (20 ng) cDNA template, 0.4 μl (0.2 μM) sense primer, 0.4 μl (0.2 μM) anti-sense primer, and 8.2 μl nuclease-free water. Primers ( Supp Table S1 [online only] ) used for qPCR were designed using the BatchPrimer3 program ( https://probes.pw.usda.gov/batchprimer3/ ), and two housekeeping genes ( β-actin and 18S rRNA ) were used as references to normalize target gene expression ( Liu et al 2017b ).…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization and Expression Analysis of Two Acetylcholinesterase Genes From the Small White Butterfly Pieris rapae (Lepidoptera: Pieridae)

Jiang

Liu

2018

Journal of Insect Science

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Acetylcholinesterases (AChEs) are essential for the hydrolysis of the neurotransmitter acetylcholine and play crucial roles in the termination of neurotransmission. AChEs are encoded by the ace genes. However, the ace genes from the small white butterfly, Pieris rapae (L.) (Lepidoptera: Pieridae), remained uncharacterized. In this study, two aces (Prace1 and Prace2) were identified from P. rapae. Prace1 encoded a PrAChE1 protein consisting of 694 amino acid residues, and Prace2 encoded the 638-amino-acid PrAChE2. The two identified PrAChEs both had features typical of AChEs, including the catalytic triad, choline-binding sites, an oxyanion hole, an acyl pocket, a peripheral anionic subsite, an FGESAG motif and 14 conserved aromatic amino acids. Phylogenetic analysis showed that Prace1 and Prace2 were clustered into two distinct groups: ace1 and ace2, respectively. The two Praces were distributed on different genomic scaffolds: Prace1 on scaffold 156 and Prace2 on scaffold 430. Additionally, Prace1 consisted of three exons and two introns, whereas Prace2 consisted of six exons and five introns. One amino acid mutation (Gly324Ala) in PrAChE1 and two (Ser291Gly and Ser431Phe) in PrAChE2 were consistent with mutations in other insect AChEs that are associated with insecticide insensitivity. Both Prace1 and Prace2 were highly expressed at the fifth-instar larval stage and in the larval head, and the transcriptional levels of Prace1 were significantly higher than those of Prace2 in all of the tested life stages and tissues. This is the first report characterizing two ace genes in P. rapae. The results pave the way for functional study of these genes.

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De novo transcriptomic analysis to reveal insecticide action and detoxification-related genes of the predatory bug, Cyrtorhinus lividipennis

Cited by 5 publications

References 41 publications

Chromosome‐level genome assembly of the mirid predator Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae), an important natural enemy in the rice ecosystem

Chromosome‐level genome assembly of the mirid predator Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae), an important natural enemy in the rice ecosystem

Detrimental Impact of λ-Cyhalothrin on the Biocontrol Efficacy of Eocanthecona furcellata by Affecting Global Transcriptome and Predatory Behavior

Molecular Characterization and Expression Analysis of Two Acetylcholinesterase Genes From the Small White Butterfly Pieris rapae (Lepidoptera: Pieridae)

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