Deaminative-cleaved S. monotuberculatus fucosylated glycosaminoglycan: Structural elucidation and anticoagulant activity

Yuan, Qiuyan; Li, Hong; Wang, Qian; Sun, Shujing; Fang, Ziyu; Tang, Hao; Shi, Xiaohuo; Wen, Jing; Huang, Lianghua; Meng, Bo; Zhong, Shaobin; Liu, Yonghong; Gao, Chenghai; Zhao, Longyan

doi:10.1016/j.carbpol.2022.120072

Cited by 11 publications

(8 citation statements)

References 41 publications

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“…The EC 50 value of TPGS for inhibiting intrinsic tenase (FXase) was 77.15 ng/mL, which was close to that of LMWH (69.82 ng/mL). LMWH showed strong AT-dependent anti-FXa and anti-FIIa activities with EC 50 values of 61.49 and 46.07 ng/mL, respectively, which was consistent with a previous study [ 34 ]. However, TPGS showed no AT-dependent anti-FIIa and anti-FXa activities (EC 50 > 2000 ng/mL).…”

Section: Resultssupporting

confidence: 92%

“…The extraction of polysaccharides from T. palmiferus was based on our previous method, with some modifications [ 34 ]. The brittle stars were dried in an oven at 60 °C and then pulverized into a uniform powder.…”

Section: Methodsmentioning

confidence: 99%

“…The molecular weight was determined by using a Shimadzu LC-2030C 3D HPLC apparatus (Shimadzu Corp., Kyoto, Japan) with a refractive index RID detector and analyzed by a Shodex OHpak SB-804 HQ column (7 µm, 8 × 300 mm) [ 34 ]. The column temperature was maintained at 35 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The inhibition of intrinsic FXase was measured by using the BIOPHEN FVIII: C kit according to our previous study [ 34 ]. Briefly, 30 μL of the polysaccharide solution at a gradient concentration, 2 IU/mL factor VIII (30 μL), and 60 nM factor IXa (30 μL) (containing FIIa, Ca 2+ , and PC/PS) were added to the 96-well plate and incubated for 2 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

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Structural Elucidation of a Glucan from Trichaster palmiferus by Its Degraded Products and Preparation of Its Sulfated Derivative as an Anticoagulant

Yuan

Tang

et al. 2023

Marine Drugs

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Echinoderms have been attracting increasing attention for their polysaccharides, with unique chemical structure and enormous potential for preparing drugs to treat diseases. In this study, a glucan (TPG) was obtained from the brittle star Trichaster palmiferus. Its structure was elucidated by physicochemical analysis and by analyzing its low-molecular-weight products as degraded by mild acid hydrolysis. The TPG sulfate (TPGS) was prepared, and its anticoagulant activity was investigated for potential development of anticoagulants. Results showed that TPG consisted of a consecutive α1,4–linked D-glucopyranose (D-Glcp) backbone together with a α1,4–linked D-Glcp disaccharide side chain linked through C−1 to C-6 of the main chain. The TPGS was successfully prepared with a degree of sulfation of 1.57. Anticoagulant activity results showed that TPGS significantly prolonged activated partial thromboplastin time, thrombin time, and prothrombin time. Furthermore, TPGS obviously inhibited intrinsic tenase, with an EC50 value of 77.15 ng/mL, which was comparable with that of low-molecular-weight heparin (LMWH) (69.82 ng/mL). TPGS showed no AT-dependent anti-FIIa and anti-FXa activities. These results suggest that the sulfate group and sulfated disaccharide side chains play a crucial role in the anticoagulant activity of TPGS. These findings may provide some information for the development and utilization of brittle star resources.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Structural Elucidation of a Glucan from Trichaster palmiferus by Its Degraded Products and Preparation of Its Sulfated Derivative as an Anticoagulant

Yuan

Tang

et al. 2023

Marine Drugs

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“…Stichopus monotuberculatus is another well-known tropical sea cucumber used in folk medicine and tonic food with the traditional belief of its bioactive properties. However, in a recent study Yuan et al (2022) scientific evidence for its anticoagulant activities using fucosylated glycosaminoglycan. According to the authors, fucosylated glycosaminoglycan had the potential to selectively inhibit intrinsic tenase complex without showing any effects on anti-factor IIa, Xa, and VIIa activity under the tested conditions (Yuan et al, 2022).…”

Section: Anticoagulant Activities Reported From Sea Cucumbersmentioning

confidence: 99%

Bioactive secondary metabolites in sea cucumbers and their potential to use in the functional food industry

Sanjeewa¹,

Herath²

2023

Fish Aquat Sci

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The bioactive secondary metabolites produced by sea cucumbers are very diverse with differences in composition, linkages, molecular weight, and various functional properties. Due to their physicochemical properties, these bioactive molecules in sea cucumbers have found applications in various market segments such as functional foods and cosmetics. Sea cucumber side dishes are a prominent food item in traditional cuisine in East Asian countries such as South Korea, China, and Japan. In addition, many studies have reported that the consumption of sea cucumbers can reduce the risk of cardiovascular disease, the pathogenesis of cancer cells, chronic inflammatory diseases, etc. In particular, many studies have recently reported the potential of sea cucumbers to develop functional products to reduce inflammation, oxidative stress, diabetes, and cancer. Additionally, these bioactive properties associated with sea cucumbers make them ideal compounds for use as functional ingredients in functional food products. However, no report has yet reviewed the properties of sea cucumbers related to functional foods. Therefore, in this review, the primary focus is given to collecting published scientific data (from 2019 to 2023) on the bioactive properties of sea cucumbers relevant to the functional food industry.

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Degradation of chondroitin sulfate: Mechanism of degradation, influence factors, structure-bioactivity relationship and application

Wang

Qi²,

Zhao³

et al. 2023

Carbohydrate Polymers

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Deaminative-cleaved S. monotuberculatus fucosylated glycosaminoglycan: Structural elucidation and anticoagulant activity

Cited by 11 publications

References 41 publications

Structural Elucidation of a Glucan from Trichaster palmiferus by Its Degraded Products and Preparation of Its Sulfated Derivative as an Anticoagulant

Structural Elucidation of a Glucan from Trichaster palmiferus by Its Degraded Products and Preparation of Its Sulfated Derivative as an Anticoagulant

Bioactive secondary metabolites in sea cucumbers and their potential to use in the functional food industry

Degradation of chondroitin sulfate: Mechanism of degradation, influence factors, structure-bioactivity relationship and application

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