Debneyol, a fungicidal sesquiterpene from tnv infected Nicotiana debneyi

Burden, Raymond S.; Rowell, P.M.; Bailey, J. A.; Loeffler, R. S. Thomas; Kemp, Malcolm S.; Brown, Charles A.

doi:10.1016/s0031-9422(00)83008-3

Cited by 30 publications

(19 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specific silencing of either Nb-ABCG1 or Nb-ABCG2 had no significant effect on capsidiol accumulation (Supplemental Figure 8C). Accumulation of debneyol, another sesquiterpenoid phytoalexin structurally related to capsidiol (Burden et al, 1985), was also reduced in Nb-ABCG1/2-silenced plants (Supplemental Figure 11). As degradation of the sesquiterpenoid phytoalexins rishitin and capsidiol by potato (Solanum tuberosum) and sweet pepper (Capsicum annuum) cells has been reported previously (Stöessl et al, 1977;Horikawa et al, 1976), it was conceivable that capsidiol and debneyol may be degraded or metabolized in cells of Nb-ABCG1/2-silenced plants.…”

Section: Effect Of Nb-abcg1/2 Silencing On the Accumulation Of Phytoamentioning

confidence: 97%

The Full-Size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 Function in Pre- and Postinvasion Defense against Phytophthora infestans in Nicotiana benthamiana

et al. 2016

View full text Add to dashboard Cite

Section: Effect Of Nb-abcg1/2 Silencing On the Accumulation Of Phytoamentioning

confidence: 97%

The Full-Size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 Function in Pre- and Postinvasion Defense against Phytophthora infestans in Nicotiana benthamiana

et al. 2016

View full text Add to dashboard Cite

“…4A). The P_ Bccpdh:GFP transformant was incubated with other anti-microbial terpenoids produced by Solanaceae species, including rishitin, debneyol (29), sclareol (30) and capsidiol 3-acetate (31). Among the terpenoids tested, treatment of capsidiol and capsidiol 3-acetate induced expression of GFP in the P_ Bccpdh:GFP transformant.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Botrytis cinerea identifies host plants via the recognition of antifungal capsidiol to induce expression of a specific detoxification gene

Kuroyanagi

Bulasag

Fukushima

et al. 2022

Preprint

View full text Add to dashboard Cite

The gray mold pathogen Botrytis cinerea has a broad host range, causing disease in over 400 plant species, but it is not known how this pathogen evolved this polyxenous nature. B. cinerea can metabolize a wide range of phytoalexins, including the stilbenoid, resveratrol, and the sesquiterpenoids capsidiol in tobacco, and rishitin in potato and tomato. In this study, we analyzed the metabolism of sesquiterpenoid phytoalexins by B. cinerea. Capsidiol was dehydrogenated to capsenone which was then further oxidized, while rishitin was directly oxidized to epoxy- or hydroxy-rishitins indicating that B. cinerea has separate mechanisms to detoxify structurally similar sesquiterpenoid phytoalexins. RNAseq analysis revealed that a distinct set of genes were induced in B. cinerea when treated with capsidiol or rishitin, suggesting that B. cinerea can distinguish structurally similar phytoalexins to activate appropriate detoxification mechanisms. The gene most highly upregulated by capsidiol treatment encoded a dehydrogenase, designated Bccpdh. Heterologous expression of Bccpdh in a capsidiol-sensitive plant symbiotic fungus, Epichloë festucae, resulted in an acquired tolerance of capsidiol and the ability to metabolize capsidiol to capsenone, while B. cinerea Δbccpdh mutants became relatively sensitive to capsidiol. The Δbccpdh mutant showed reduced virulence on the capsidiol producing Nicotiana and Capsicum species but remained fully pathogenic on potato and tomato. Homologs of Bccpdh are found in taxonomically distant Ascomycota fungi but not in other Botrytis species, suggesting that B. cinerea acquired the ancestral Bccpdh by horizontal gene transfer, thereby extending the pathogenic host range of this polyxenous pathogen to capsidiol-producing plant species.Significance StatementB. cinerea can metabolize a wide range of phytoalexins, however, the extent to which phytoalexin detoxification contributes to pathogenicity is largely unknown. In this study, we have shown that B. cinerea recognizes structurally resembling sesquiterpenoid phytoalexins, rishitin and capsidiol, to activate appropriate detoxification mechanisms. We identify Bccpdh, encoding a dehydrogenase for capsidiol detoxification, which is upregulated in B. cinerea exclusively during the infection of capsidiol producing plant species, and is required to exert full virulence. Analysis of the Bccpdh locus suggests that the gene was acquired via horizontal gene transfer. This work highlights that the polyxenous plant pathogen B. cinerea can distinguish its host plants by its anti-microbial compounds, to activate appropriate mechanisms for enhanced virulence.

show abstract

“…Similarly, systemic resistance to anthracnose in cucumber is induced by inoculation with TMV (Jenns and Kuć, 1980), as is resistance to Peronospora tabacina in tobacco (Ye et al, 1989) and Erysiphe chicoracearum (Marte et al, 1993). Indeed, fungicidal compounds have been isolated from plants reacting with necrosis to virus infection (Burden et al, 1985). The development of resistance of this sort probably involves the PR proteins discussed in Chapter 11, Section III.…”

Section: Effect Of Virus Infection On Fungal Diseases a Increased Rementioning

confidence: 99%

Symptoms and Host Range

Hull

2014

Plant Virology

View full text Add to dashboard Cite

Debneyol, a fungicidal sesquiterpene from tnv infected Nicotiana debneyi

Cited by 30 publications

References 30 publications

The Full-Size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 Function in Pre- and Postinvasion Defense against Phytophthora infestans in Nicotiana benthamiana

The Full-Size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 Function in Pre- and Postinvasion Defense against Phytophthora infestans in Nicotiana benthamiana

Botrytis cinerea identifies host plants via the recognition of antifungal capsidiol to induce expression of a specific detoxification gene

Symptoms and Host Range

Contact Info

Product

Resources

About