Decalactone Production by Yarrowia lipolytica under increased O2 Transfer Rates

Aguedo, Mário; Gomes, Nelma; Garcia, Erandi Escamilla; Waché, Yves; Мота, М.; Teixeira, J. A.; Belo, Isabel

doi:10.1007/s10529-005-2517-z

Cited by 57 publications

(47 citation statements)

References 13 publications

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“…For the highest fractions of Tween 80 and MR tested, which correspond to the biotransformation medium composition usually used [29], k L a values are smaller than those observed with smaller fractions, as it can be seen in Fig. 2A and D.…”

Section: Effect Of the Biphasic Medium On K L A Valuesmentioning

confidence: 67%

Oxygen mass transfer in a biphasic medium: Influence on the biotransformation of methyl ricinoleate into γ-decalactone by the yeast Yarrowia lipolytica

Gomes

Aguedo

Teixeira

et al. 2007

Biochemical Engineering Journal

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In this work, an empirical correlation is proposed to describe k L a as a function of operating conditions (agitation and aeration rates) and of oil and surfactant volumetric fractions in a biotransformation medium, an oil-in-water dispersion. An interaction effect between the oil and the surfactant effects was found, since oil presence increased k L a in the absence of the surfactant but had an opposite effect when Tween 80 was available in the medium. The biotransformation of methyl ricinoleate (MR) into ␥-decalactone (an aroma compound of industrial interest), by the yeast Yarrowia lipolytica, was carried out at different conditions of operation, to evaluate the influence of k L a on the production of the aroma. It was demonstrated that k L a had an influence on the aroma production; however, for the low hydrophobic substrate concentration used (1.08% v/v) and cellular density of 2.0 × 10 7 cells mL −1 , a minimal k L a value of 70 h −1 was necessary to attain the maximal aroma production.

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Section: Effect Of the Biphasic Medium On K L A Valuesmentioning

confidence: 67%

Oxygen mass transfer in a biphasic medium: Influence on the biotransformation of methyl ricinoleate into γ-decalactone by the yeast Yarrowia lipolytica

Gomes

Aguedo

Teixeira

et al. 2007

Biochemical Engineering Journal

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“…x 0 =3 mm) (exp. IV in Figure 3) was reduced to about 50% of that obtained in traditional scale-down systems, such as ST bioreactors (Aguedo et al 2005) or shake flasks. This was confirmed with further experiments carried out at the same conditions (data not shown).…”

Section: The Experimental Biotransformation Set-upmentioning

confidence: 85%

“…In the present case this is beneficial to the metabolism of Y. lipolytica which is a strictly aerobic yeast species. O 2 also indirectly participates in the metabolic pathway leading to the formation of the lactone (peroxisomal b-oxidation), however, no direct relation between oxygen supply and the rate of c-decalactone production was evidenced (Aguedo et al 2005).…”

Section: The Experimental Biotransformation Set-upmentioning

confidence: 99%

Application of a Novel Oscillatory Flow Micro-bioreactor to the Production of γ-decalactone in a Two Immiscible Liquid Phase Medium

et al. 2006

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A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of c-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred tank bioreactor or shake flask). A three-fold increase in c-decalactone productivity was obtained by increasing oscillatory mixing intensity from Re o $482 to Re o $1447. This was presumably related to the effective contribution of the reactor geometry to enhanced mass transfer rates between the two immiscible liquid phases involved in the process by increasing the interfacial area.

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“…The biotechnological production of c-decalactone, a fruity-like aroma compound, results from the biotransformation of ricinoleic acid and it can be carried out by several microorganisms, among which the dimorphic yeast Yarrowia lipolytica (Aguedo et al 2005a).…”

Section: Introductionmentioning

confidence: 99%

Oil-in-water emulsions characterization by laser granulometry and impact on γ-decalactone production in Yarrowia lipolytica

et al. 2011

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Oil-in-water emulsions composed of methyl ricinoleate (MR) or castor oil (CO) as the organic phase, stabilized by Tween 80, are in the basis of the biotechnological production of γ-decalactone. Yarrowia lipolytica was used due to its ability to grow on hydrophobic substrates and to carry out the biotransformation. The characterization of oil droplets size distribution by laser granulometry was performed under different oil concentrations. The impact of the presence of cells on droplets size was also analyzed as well as the relevance of washing inoculum cells. Furthermore, the granulometric characterization of the emulsions was related with γ-decalactone production and it was observed that, in the presence of non-washed cells, the smaller droplets disappeared, using both oils, which increased γ-decalactone concentration. This suggests that the access of cells to the substrate occurs by their adhesion around larger oil droplets.

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Decalactone Production by Yarrowia lipolytica under increased O2 Transfer Rates

Cited by 57 publications

References 13 publications

Oxygen mass transfer in a biphasic medium: Influence on the biotransformation of methyl ricinoleate into γ-decalactone by the yeast Yarrowia lipolytica

Oxygen mass transfer in a biphasic medium: Influence on the biotransformation of methyl ricinoleate into γ-decalactone by the yeast Yarrowia lipolytica

Application of a Novel Oscillatory Flow Micro-bioreactor to the Production of γ-decalactone in a Two Immiscible Liquid Phase Medium

Oil-in-water emulsions characterization by laser granulometry and impact on γ-decalactone production in Yarrowia lipolytica

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