2014
DOI: 10.1016/j.jconrel.2014.08.028
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Decationized polyplexes as stable and safe carrier systems for improved biodistribution in systemic gene therapy

Abstract: Many polycation-based gene delivery vectors show high transfection in vitro, but their cationic nature generally leads to significant toxicity and poor in vivo performance which significantly hampers their clinical applicability. Unlike conventional polycation-based systems, decationized polyplexes are based on hydrophilic and neutral polymers. They are obtained by a 3-step process: charge-driven condensation followed by disulfide crosslinking stabilization and finally polyplex decationization. They consist of… Show more

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Cited by 36 publications
(34 citation statements)
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References 75 publications
(125 reference statements)
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“…Furthermore, the neutral block copolymer pHP--PEG that forms decationized polyplexes showed excellent cytocompatibility upon incubation with HUVEC cells. Finally, an in vivo zebrafish nanotoxicity assay [21] also demonstrated that this polymer had a remarkable low teratogenicity and mortality profile, in great contrast with its biodegradable cationic counterpart ( Figure 2B) [22].…”
Section: Decationized Polyplexes Propertiesmentioning
confidence: 94%
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“…Furthermore, the neutral block copolymer pHP--PEG that forms decationized polyplexes showed excellent cytocompatibility upon incubation with HUVEC cells. Finally, an in vivo zebrafish nanotoxicity assay [21] also demonstrated that this polymer had a remarkable low teratogenicity and mortality profile, in great contrast with its biodegradable cationic counterpart ( Figure 2B) [22].…”
Section: Decationized Polyplexes Propertiesmentioning
confidence: 94%
“…The in vivo applicability of decationized polyplexes for systemic administration was evaluated by determining the stability of decationized polyplexes in full human plasma and a notably stable size distribution was observed for 48 h, whereas their cationic counterpart showed some degree of aggregation [22]. Polyplexes were also evaluated for their Figure 1.…”
Section: Decationized Polyplexes Propertiesmentioning
confidence: 99%
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“…[16,17] The successful employment of siRNA to conduct gene therapy relies on the development of safe and efficient vectors. [18] Polyethyleneimine (PEI) is one of the most effective cationic polymers for gene delivery and has been employed to construct many advanced vectors. [19,20] In addition, gold nanoparticles (GNPs) have recently emerged as an attractive candidate for delivery of various payloads, including drug and gene.…”
Section: Introductionmentioning
confidence: 99%