Decellularization of tissues and organs

Cesur, Nevra Pelin; Yalman, Volkan; Türkoğlu, Nelisa

doi:10.7197/cmj.vi.609592

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…Similarly, histological staining indicated almost the same trend in inflammation, dermal cell proliferation, and angiogenesis (Figure 9). Some previous studies reported the same trend, 61,62 while others showed the unintended effects of enzymatic steps on wound healing. The trypsin enzyme cleaves protein subsets through disulfide bonds which eventually causes wound healing postponement and scar tissue formation 63 .…”

Section: Discussionsupporting

confidence: 52%

The effect of source animal age, decellularization protocol, and sterilization method on bovine acellular dermal matrix as a scaffold for wound healing and skin regeneration

et al. 2022

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Background: Healing the full-thickness skin wounds has remained a challenge.One of the most frequently used grafts for skin regeneration is xenogeneic acellular dermal matrices (ADMs), including bovine ADMs. This study investigated the effect of the source animal age, enzymatic versus non-enzymatic decellularization protocols, and gamma irradiation versus ethylene oxide (EO) sterilization on the scaffold. Methods: ADMs were prepared using the dermises of fetal bovine or calf skins.All groups were decellularized through chemical and mechanical methods, unless T-FADM samples, in which an enzymatic step was added to the decellularization protocol. All groups were sterilized with ethylene oxide (EO), except G-FADM which was sterilized using gamma irradiation. The scaffolds were characterized through scanning electron microscopy, differential scanning calorimetry, tensile test, MTT assay, DNA quantification, and real-time PCR. The performance of the ADMs in wound treatment was also evaluated macroscopically and histologically.Results: All ADMs were effectively decellularized. In comparison to FADM (EOsterilized fetal ADM), morphological, and mechanical properties of G-FADM, T-FADM, and CADM (EOsterilized calf ADM) were changed to different extents. In addition, the CADM and G-FADM were thermally more stable than the FADM and T-FADM. Although all ADMs were noncytotoxic, the wounds of the FADM, T-FADM, and G-FADM groups were contracted to almost 30.0% of the original area on day 7, significantly faster than the CADM (17.5% ± 1.7) and control (12.2% ± 1.59) groups. However, by day 21, all ADMs were mostly closed except for the untreated group (60.1 ± 1.8). Conclusion:Altogether, fetal source and EO-sterilized samples performed better than calf source and gamma-sterilized samples unless in some mechanical properties. There was no added value in using enzymatic treatment during the decellularization process. Our results suggest that the age, decellularization, and

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Section: Discussionsupporting

confidence: 52%

The effect of source animal age, decellularization protocol, and sterilization method on bovine acellular dermal matrix as a scaffold for wound healing and skin regeneration

et al. 2022

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“…The elimination of cellular content from natural tissue-derived matrices has the potential to promote healing, integration with host tissues, and limit a foreign body reaction [107]. The decellularization process typically involves the use of chemicals, enzymes, physical forces, and/or a combination of these methods to remove cellular components, while preserving the ECM [108,109]. Methods of decellularization affect the structure of the ECM, the structure of the tissue, and the biomechanical behavior.…”

Section: Decellularizationmentioning

confidence: 99%

Placental-Derived Biomaterials and Their Application to Wound Healing: A Review

Protzman¹,

Mao

Long

et al. 2023

Bioengineering

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Chronic wounds are associated with considerable patient morbidity and present a significant economic burden to the healthcare system. Often, chronic wounds are in a state of persistent inflammation and unable to progress to the next phase of wound healing. Placental-derived biomaterials are recognized for their biocompatibility, biodegradability, angiogenic, anti-inflammatory, antimicrobial, antifibrotic, immunomodulatory, and immune privileged properties. As such, placental-derived biomaterials have been used in wound management for more than a century. Placental-derived scaffolds are composed of extracellular matrix (ECM) that can mimic the native tissue, creating a reparative environment to promote ECM remodeling, cell migration, proliferation, and differentiation. Reliable evidence exists throughout the literature to support the safety and effectiveness of placental-derived biomaterials in wound healing. However, differences in source (i.e., anatomical regions of the placenta), preservation techniques, decellularization status, design, and clinical application have not been fully evaluated. This review provides an overview of wound healing and placental-derived biomaterials, summarizes the clinical results of placental-derived scaffolds in wound healing, and suggests directions for future work.

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“…The elimination of cellular content from natural tissue-derived matrices has the potential to promote healing, integration with host tissues, and limit a foreign body reaction [96]. The decellularization process typically involves the use of chemicals, enzymes, physical forces, and/or a combination of these methods to remove cellular components, while preserving the ECM [97,98]. Methods of decellularization affect the structure of the ECM, the structure of the tissue, and the biomechanical behavior.…”

Section: Decellularizationmentioning

confidence: 99%

Placental-Derived Biomaterials and Their Application to Wound Healing: A Review

Protzman¹,

Mao²,

Long³

et al. 2023

Preprint

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Chronic wounds are associated with considerable patient morbidity and present a significant economic burden to the healthcare system. Often, chronic wounds are in a state of persistent in-flammation and unable to progress to the next phase of wound healing. Placental-derived bio-materials are recognized for their biocompatibility, biodegradability, angiogenic, an-ti-inflammatory, anti-microbial, anti-fibrotic, immunomodulatory, and immune privileged prop-erties. As such, placental-derived biomaterials have been used in wound management for more than a century. Placental-derived scaffolds are composed of an extracellular matrix (ECM) that can mimic the native tissue, creating a reparative environment to promote ECM remodeling, cell migration, proliferation, and differentiation. Reliable evidence exists throughout the literature to support the safety and effectiveness of placental-derived biomaterials in wound healing. How-ever, differences in source (i.e., anatomical regions of the placenta), preservation techniques, decellularization status, design, and clinical application have not been fully evaluated. This re-view provides an overview of wound healing and placental-derived biomaterials, summarizes the clinical results of placental-derived scaffolds in wound healing, and suggests directions for future work.

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Decellularization of tissues and organs

Cited by 3 publications

References 23 publications

The effect of source animal age, decellularization protocol, and sterilization method on bovine acellular dermal matrix as a scaffold for wound healing and skin regeneration

The effect of source animal age, decellularization protocol, and sterilization method on bovine acellular dermal matrix as a scaffold for wound healing and skin regeneration

Placental-Derived Biomaterials and Their Application to Wound Healing: A Review

Placental-Derived Biomaterials and Their Application to Wound Healing: A Review

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