Decellularized extracellular matrix bioinks and the external stimuli to enhance cardiac tissue development in vitro

Das, Sanjeev; Kim, Seok-Won; Choi, Yeong‐Jin; Lee, Sooyeon; Lee, Se-Hwan; Kong, Jeong-Sik; Park, Hun‐Jun; Cho, Dong‐Woo; Jang, Jinah

doi:10.1016/j.actbio.2019.04.026

Cited by 124 publications

(84 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various types of dECM bio-inks have been introduced for potential applications in artificial tissue regeneration. [5][6][7][8][9][10] In dECM bio-ink development, detergents are commonly used in the decellularization process and are the greatest determinants of the bio-ink properties. [16][17][18][20][21][22] Despite various studies on dECM bio-inks, analyses of the effectiveness of different detergents are lacking.…”

Section: Discussionmentioning

confidence: 99%

“…Various types of animal tissue-derived dECM bio-inks have been introduced. [4][5][6][7] Pati et al 8 reported that dECM bio-inks derived from the porcine heart, cartilage, and adipose tissue exhibit excellent performance in tissue-specific differentiation. Yi et al 9 introduced a tumor model printed with glioblastoma-derived dECM bio-ink that produces a patient-specific drug response.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

2021

View full text Add to dashboard Cite

Decellularized extracellular matrix-based bio-inks (dECM bio-inks) for bioprinting technology have recently gained attention owing to their excellent ability to confer tissue-specific functions and 3D-printing capability. Although decellularization has led to a major advancement in bio-ink development, the effects of detergent type, the most important factor in decellularization, are still unclear. In this study, the effects of various detergent types on bio-ink performance were investigated. Porcine liver-derived dECM bio-inks prepared using widely used detergents, including sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), Triton X-100 (TX), and TX with ammonium hydroxide (TXA), were characterized in detail. SDS and SDC severely damaged glycosaminoglycan and elastin proteins, TX showed the lowest rate of decellularization, and TXA-based dECM bio-ink possessed the highest ECM content among all bio-inks. Differences in biochemical composition directly affected bio-ink performance, with TXA-dECM bio-ink showing the best performance with respect to gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. More importantly, cytocompatibility tests using primary mouse hepatocytes also showed that the TXA-dECM bio-ink improved albumin secretion and cytochrome P450 activity by approximately 2.12- and 1.67-fold, respectively, compared with the observed values for other bio-inks. Our results indicate that the detergent type has a great influence on dECM damage and that the higher the dECM content, the better the performance of the bio-ink for 3D bioprinting.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

2021

View full text Add to dashboard Cite

show abstract

“…In vivo, the printed GelMA-cardiac ECM patches remained attached to rat hearts epicardially, and vessels were formed after 14 days, indicating their integration with the native myocardium [83]. The importance of cardiac ECM as a component of bioinks was also underlined by Das and colleagues, who created bioinks with porcine heart tissue-derived ECM or collagen for encapsulating neonatal rat CMs (2 × 10 7 cells/mL) using an extrusion-based 3D bioprinter [84]. The patches were then cultured for a month in dynamic or static conditions with the aim to evaluate the structural arrangement of CMs and their subsequent gene expression.…”

Section: D Bioprinting Of Functional Myocardiummentioning

confidence: 96%

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

Gardin

Ferroni

Latrémouille

et al. 2020

Cells

View full text Add to dashboard Cite

Three dimensional (3D) printing, which consists in the conversion of digital images into a 3D physical model, is a promising and versatile field that, over the last decade, has experienced a rapid development in medicine. Cardiovascular medicine, in particular, is one of the fastest growing area for medical 3D printing. In this review, we firstly describe the major steps and the most common technologies used in the 3D printing process, then we present current applications of 3D printing with relevance to the cardiovascular field. The technology is more frequently used for the creation of anatomical 3D models useful for teaching, training, and procedural planning of complex surgical cases, as well as for facilitating communication with patients and their families. However, the most attractive and novel application of 3D printing in the last years is bioprinting, which holds the great potential to solve the ever-increasing crisis of organ shortage. In this review, we then present some of the 3D bioprinting strategies used for fabricating fully functional cardiovascular tissues, including myocardium, heart tissue patches, and heart valves. The implications of 3D bioprinting in drug discovery, development, and delivery systems are also briefly discussed, in terms of in vitro cardiovascular drug toxicity. Finally, we describe some applications of 3D printing in the development and testing of cardiovascular medical devices, and the current regulatory frameworks that apply to manufacturing and commercialization of 3D printed products.Cells 2020, 9, 742 2 of 33 Cells 2020, 9, 742 3 of 33 in digital modeling softwares without the need for file type conversion [16]. Several medical image segmentation softwares are available; some of these are open-source and freely accessible, while others are commercial, licensed products. From the DICOM dataset, the target anatomic geometry is identified and segmented based on properties such as contrast or brightness [17]. As a result, segmentation masks are created such that pixels with the same intensity range are grouped and converted into 3D digital models using rendering techniques. These segmented digital models are then exported out in the standard tessellation language (STL) file type, which is the industry standard for 3D objects and 3D printing software [17]. Nevertheless, STL files do not contain color information and in order to print in multiple colors, a different file format will need to be used. For example, virtual reality modeling language (VRML) and additive manufacturing file format (AMF) provide multiple color and material options [18]. Once segmentation is completed, the final 3D digital model may be further modified within computer-aided design (CAD) software. The main post-processing adjustments of the 3D model are: Repairing errors and discontinuities that sometimes arise in the image segmentation and exporting processes, smoothing of the surface of the model due to scaling errors resulting from the resolution of the original medical image, and addition of other...

show abstract

“…The contractile force readout was collected in a real-time manner [16,131]. In addition, Das et al demonstrated the promising effect of a heart-derived decellularized ECM (hdECM) bioink on accelerating the maturation of cardiac muscle tissue constructs in vitro (Figure 2c) [20]. Using a 3D bioprinting process, an in vitro model of the cardiac muscle was constructed to allow fixation of the cell-laden hdECM hydrogel on poly(ethylene/vinyl acetate) anchors.…”

Section: Cardiovascular Systemmentioning

confidence: 99%

3D Bioprinting of In Vitro Models Using Hydrogel-Based Bioinks

Choi

Park

Ha³

et al. 2021

Polymers

Self Cite

View full text Add to dashboard Cite

Coronavirus disease 2019 (COVID-19), which has recently emerged as a global pandemic, has caused a serious economic crisis due to the social disconnection and physical distancing in human society. To rapidly respond to the emergence of new diseases, a reliable in vitro model needs to be established expeditiously for the identification of appropriate therapeutic agents. Such models can be of great help in validating the pathological behavior of pathogens and therapeutic agents. Recently, in vitro models representing human organs and tissues and biological functions have been developed based on high-precision 3D bioprinting. In this paper, we delineate an in-depth assessment of the recently developed 3D bioprinting technology and bioinks. In particular, we discuss the latest achievements and future aspects of the use of 3D bioprinting for in vitro modeling.

show abstract

Decellularized extracellular matrix bioinks and the external stimuli to enhance cardiac tissue development in vitro

Cited by 124 publications

References 55 publications

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

3D Bioprinting of In Vitro Models Using Hydrogel-Based Bioinks

Contact Info

Product

Resources

About