Decellularized fish swim bladder patch loaded with mesenchymal stem cells inhibits neointimal hyperplasia

Abstract

We previously showed decellularized fish swim bladder can be used as vascular patch and tube graft in rats, mesenchymal stem cells (MSCs) have showed the capability to inhibit neointimal hyperplasia in different animal models. We hypothesized that decellularized fish swim bladder patch loaded with MSCs (bioinspired patch) can inhibit neointimal hyperplasia in a rat aortic patch angioplasty model. Rat MSCs were grown in vitro and flow cytometry was used to confirm their quality. 3.6  10 5 MSCs were mixed into 100 μl of sodium alginate (SA)/hyaluronic acid (HA) hydrogel, two layers of fish swim bladders (5 mm  5 mm) were sutured together, bioinspired patch was created by injection of hydrogel with MSCs into the space between two layers of fish swim bladder patches. Decellularized rat thoracic aorta patch was used as control. Patches were harvested at days 1 and 14 after implantation. Samples were examined by histology, immunohistochemistry, and immunofluorescence. The decellularized rat thoracic aorta patch and the fish swim bladder patch had a similar healing process after implantation. The bioinspired patch had a similar structure like native aorta. Bioinspired patch showed a decreased neointimal thickness (p = .0053), fewer macrophages infiltration (p = .0090), and lower proliferation rate (p = .0291) compared to the double layers fish swim bladder patch group. Decellularized fish swim bladder patch loaded with MSCs can inhibit neointimal hyperplasia effectively.Although this is a preliminary animal study, it may have a potential application in large animals or clinical research.

Influences of Polyphenols on the Properties of Crosslinked Acellular Fish Swim Bladders: Experiments and Molecular Dynamic Simulations

“…Li et al [ 2 ] confirmed that AFSB utilized as a dura mater substitute can meet the demands of clinical applications. Liu et al [ 3 ] prepared AFSB-based scaffolds crosslinked by glutaraldehyde (GA) for cardiovascular diseases, which showed superior anti-calcification properties and biocompatibility compared to bovine pericardium, revealing their promising application for use in cardiovascular biomaterials [ 4 , 5 , 6 ]. Furthermore, AFSBs have also shown potential for use in several fields, such as wound healing [ 7 ], cervical oesophagoplasty [ 8 ] and cystoplasty [ 9 ].…”

Preparation of extracellular matrix of fish swim bladders by decellularization with supercritical carbon dioxide

“…Fish swim bladders (FSBs) used to be considered as garbage in fishery (Yadav et al 2019 ), however, with the well-aligned natural collagen nano-fibrils, they have been applied in biomedical fields at present, such as the bio-piezoelectric nanogenerator used as a sensor (Ghosh and Mandal 2016 ). Meanwhile, after decellularization treatment, acellular fish swim bladders (AFSBs) with high content of collagen, excellent biocompatibility and biodegradable properties (Shahabipour et al 2013 ; Yazdani Moghaddam et al 2009 ), have been used as tissue engineering scaffolds in several fields (Bai et al 2021 ; Li et al 2021a , b ; Sun et al 2022 ). The outstanding advantage of scaffolds with AFSBs is that they can decrease the risk of foot and mouth disease, bovine spongiform encephalopathy and immune response when compared with mammalian-derived materials (Kim and Mendis 2006 ).…”