Decellularized laminate tissues and their derivatives as templates intended for abdominal wall regeneration

Mendoza-Novelo, Birzabith; Castellano, Laura E.; Delgado, Jorge; Quintero-Ortega, Iraís A.; Vargas-Mancilla, Juan; Vega‐González, Arturo

doi:10.1016/j.matlet.2015.11.048

Cited by 4 publications

(2 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study contributes to recognize the effect of TX and STX decellularization method on macrophage activation for the purpose of designing biological implants composed of matrix remaining the pericardium decellularization. For instance, this matrix serves as structural support for the cell attachment and the reservoir of signaling factors,, or as raw material in the manufacture of cross‐linked biological implants . This research deserves further studies to elucidate the impact of both TX and STX methods of pericardium decellularization on the in vivo biomaterial performance at medium and long terms to understand the effect of pericardial bioscaffold composition on the inflammation resolution and remodeling process.…”

Section: Discussionmentioning

confidence: 99%

The component leaching from decellularized pericardial bioscaffolds and its implication in the macrophage response

Mendoza-Novelo

Castellano

Padilla-Miranda

et al. 2016

J Biomedical Materials Res

View full text Add to dashboard Cite

The extracellular matrix molecules remaining in bioscaffolds derived from decellularized xenogeneic tissues appear to be important for inducing cell functions conducting tissue regeneration. Here, we studied whether decellularization methods, that is, detergent Triton X-100 (TX) alone and TX combined with reversible alkaline swelling (STX), applied to bovine pericardial tissue, could affect the bioscaffold components. The in vitro macrophage response, subdermal biodegradation, and cell infiltration were also studied. The results indicate a lower leaching of fibronectin, but a higher leaching of laminin and sulfated glycosaminoglycans from tissues decellularized with STX and TX, respectively. The in vitro secretion of interleukin-6 and monocyte chemoattractant protein by RAW264.7 macrophages is promoted by decellularized bioscaffold leachates. A lower polymorphonuclear cell density is observed around decellularized bioscaffolds at 1-day implantation; concurrently showing a higher cell infiltration in STX- than in TX-implant. Cells infiltrated into TX-implant show a fibroblastic morphology at 7-day implantation, concurrently the capillary formation is observed at 14-day. Pericardial bioscaffolds suffer biodegradation more pronounced in STX- than in TX-implant. Both TX and STX decellularization methods favor a high leaching of basal lamina components, which presumably promotes a faster macrophage stimulation compared to nondecellularized tissue, and appear to be associated with an increased host cell infiltration in a rat subdermal implantation. Meanwhile, the connective tissue components leaching from TX decellularized bioscaffolds, unlike the STX ones, appear to be associated with an enhanced angiogenesis accompanied by an early-promoted fibroblastic cell transition. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2810-2822, 2016.

show abstract

Section: Discussionmentioning

confidence: 99%

The component leaching from decellularized pericardial bioscaffolds and its implication in the macrophage response

Mendoza-Novelo

Castellano

Padilla-Miranda

et al. 2016

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Natural extracellular matrix (ECM) is widely used in cellular and tissue engineering in various forms after different biomanufacturing processes. A variety of ECM-based materials or tissue constructs have been fabricated with the intent to regenerate tissues such as abdominal wall, heart valves, muscle, blood vessel, and trachea . ECM is a complex network that determines the structure and function of every tissue within the body .…”

Section: Introductionmentioning

confidence: 99%

Natural Extracellular Matrix for Cellular and Tissue Biomanufacturing

Xing

Qian

Jia

et al. 2016

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Natural extracellular matrices (ECM) derived from native tissues or cultured cells are extensively employed to fabricate biocompatible scaffolds or living tissue constructs for the application in cellular and tissue engineering. The composition and structure of ECM are not only heterogeneous, but also tissue or cell specific. Recapitulating the unique cell or tissue niche, ECM-based products are promising to quickly integrate with host tissues and accelerate restoration of tissue function. A variety of natural ECM-based scaffolds and tissue constructs have been biomanufactured using different approaches. Native tissue derived ECM is typically grounded into powders that can be further processed into hybrid composites in the form of hydrogels, foams, nanofibers, and 3D-printed complex constructs. Cell-derived ECM follows different biomanufacturing methods. Usually, cells are seeded on a scaffold to deposit ECM resulting in ECM-ornamented materials. The employment of resolvable scaffolds and cell sheet engineering technique enables production of complex 3D constructs exclusively composed of ECM with/without cells. In order to enhance mechanical strength, in vivo stability, and biological performance of ECM-based products, cross-linking reagents or bioactive factors are often used for modification. The major focus of this article is to provide an overview of current biomanufacturing approaches that utilize either native tissue or cell-derived natural ECM in the field of cellular and tissue engineering. Furthermore, the existing challenges for translational application of ECM-based products and the potential resolutions are discussed.

show abstract

Evaluation methods for mechanical biocompatibility of hernia repair meshes: respective characteristics, application scope and future perspectives

Cao

Gan

et al. 2021

Journal of Materials Research and Technology

View full text Add to dashboard Cite

Decellularized laminate tissues and their derivatives as templates intended for abdominal wall regeneration

Cited by 4 publications

References 71 publications

The component leaching from decellularized pericardial bioscaffolds and its implication in the macrophage response

The component leaching from decellularized pericardial bioscaffolds and its implication in the macrophage response

Natural Extracellular Matrix for Cellular and Tissue Biomanufacturing

Evaluation methods for mechanical biocompatibility of hernia repair meshes: respective characteristics, application scope and future perspectives

Contact Info

Product

Resources

About