Decellularized liver extracellular matrix and thrombin loaded biodegradable TOCN/Chitosan nanocomposite for hemostasis and wound healing in rat liver hemorrhage model

Mahbub, Md Sowaib Ibne; Bae, Sang Ho; Gwon, Jaegyoung; Lee, Byong‐Taek

doi:10.1016/j.ijbiomac.2022.11.209

Cited by 16 publications

(13 citation statements)

References 49 publications

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“…These results confirmed that GCSF-3/CT could serve as a biodegradable hemostatic material with no significant short- or long-term toxicity. This is because chitosan and silk fibroin are the primary raw materials for the preparation of GCSF-3/CT and are prone to hydrolysis by reaction with lysozymes [ 32 , 33 ]. Moreover, its porous structure endowed GCSF-3/CT with the ability to absorb a large proportion of adjacent tissue fluid.…”

Section: Resultsmentioning

confidence: 99%

Bioinspired Hemostatic Strategy via Pulse Ejections for Severe Bleeding Wounds

Ding

et al. 2023

Research

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Efficient hemostasis during emergency trauma with massive bleeding remains a critical challenge in prehospital settings. Thus, multiple hemostatic strategies are critical for treating large bleeding wounds. In this study, inspired by bombardier beetles to eject toxic spray for defense, a shape-memory aerogel with an aligned microchannel structure was proposed, employing thrombin-carrying microparticles loaded as a built-in engine to generate pulse ejections for enhanced drug permeation. Bioinspired aerogels, after contact with blood, can rapidly expand inside the wound, offering robust physical barrier blocking, sealing the bleeding wound, and generating a spontaneous local chemical reaction causing an explosive-like generation of CO 2 microbubbles, which provide propulsion thrust to accelerate burst ejection from arrays of microchannels for deeper and faster drug diffusion. The ejection behavior, drug release kinetics, and permeation capacity were evaluated using a theoretical model and experimentally demonstrated. This novel aerogel showed remarkable hemostatic performance in severely bleeding wounds in a swine model and demonstrated good degradability and biocompatibility, displaying great potential for clinical application in humans.

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Section: Resultsmentioning

confidence: 99%

Bioinspired Hemostatic Strategy via Pulse Ejections for Severe Bleeding Wounds

Ding

et al. 2023

Research

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“…The decellularized matrix can also be applied to fabricate other forms of scaffolds. For example, in the study of Mahbud and colleagues (2023), the decellularized matrix of livers was freeze-dried, ground, and combined with thrombin, oxidized cellulose, and chitosan for hemostasis and wound-healing in rat liver hemorrhage [ 32 ]. As previously mentioned, the decellularization protocol established in the present research may be employed for making different types of matrices for a variety of tissue-repair applications.…”

Section: Discussionmentioning

confidence: 99%

Easy-to-Assembly System for Decellularization and Recellularization of Liver Grafts in a Bioreactor

2023

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Decellularization of organs creates an acellular scaffold, ideal for being repopulated by cells. In this work, a low-cost perfusion system was created to be used in the process of liver decellularization and as a bioreactor after recellularization. It consists of a glass chamber to house the organ coupled to a peristaltic pump to promote liquid flow through the organ vascular tree. The rats’ liver decellularization was made with a solution of sodium dodecyl sulfate. The recellularization was made with 108 mesenchymal stromal/stem cells and cultivated for seven days. The decellularized matrices showed an absence of DNA while preserving the collagen and glycosaminoglycans quantities, confirming the efficiency of the process. The functional analyses showed a rise in lactate dehydrogenase levels occurring in the first days of the cultivation, suggesting that there is cell death in this period, which stabilized on the seventh day. Histological analysis showed conservation of the collagen web and some groups of cells next to the vessels. It was possible to establish a system for decellularization and a bioreactor to use for the recellularization method. It is easy to assemble, can be ready to use in little time and be easily sterilized.

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“…43,44 Oxidized nanocellulose has also been widely employed as wound-healing scaffolds. 45,46 Some of the most recent applications of nanocellulose modified through various oxidation routes have been summarized in Table 1.…”

Section: Surface Functionalization Of Nanocellulose Through Various C...mentioning

confidence: 99%