Decellularized Scaffolds

Background: Tissue engineering is the science of tissue design and one of the main branches of regenerative medicine that aims to improve and repair tissue injuries. Objectives: This study aimed to decellularize the tissue of bovine Achilles tendon and create a natural 3D scaffold. Then, the interaction of human adipose tissue-derived mesenchymal stem cells (hAd-MSCs) with this 3D scaffold was evaluated for use in tendon injuries. Methods: The bovine Achilles tendon was obtained from a slaughterhouse and decellularized by the combination of ethylenediaminetetraacetic acid (EDTA) and sodium dodecyl sulfate (SDS). Histological and biomechanical tests were used to evaluate the quality of decellularized scaffolds. Adipose-derived mesenchymal stem cells were cultured on scaffolds, and cell viability and cell behavior were evaluated by the MTT test and scanning electron microscopy. Results: The results of histological and biomechanical tests showed the complete removal of cells with the preservation of the extracellular matrix. The results of cell culture on scaffolds also showed that optical absorption in scaffolds containing cells increased over time. Conclusions: In general, the decellularized scaffold in this study did not undergo significant structural changes in the tendon tissue. The interaction between hAd-MSCs and the decellularized scaffold revealed that the scaffold was somehow suitable for cell culture. However, it needs to be more investigated for use in the treatment of tendon injuries of the athletes.

show abstract

“…ponents of the nucleus by detergents, and ultimately washing and removing cell debris from the extracellular matrix (3).…”

Section: R E T Rmentioning

confidence: 99%

Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Effective decellularization is necessary to prevent immune and inflammatory reactions. However, the efficiency of a given decellularization method or protocol depends on the characteristics of the tissue of interest [ 7 ]. The proposed protocol allows for the decellularization of bile ducts while preserving the ECM integrity and architecture, comparable to that of native tissue ( Figure 1 b–d).…”

Section: Discussionmentioning

confidence: 99%

“…Decellularization is a process that generates scaffolds of natural origin, with the aim to replace or repair diseased organs or tissues. During the decellularization process, most of the tissular cellular antigens that may elicit an immune response in the host body should be removed [ 7 ]. As antigens are removed, this technique may generate acellular scaffolds from both allogeneic or xenogeneic sources, thus overcoming current organ donor shortages.…”

Section: Introductionmentioning

confidence: 99%

Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility

et al. 2021

View full text Add to dashboard Cite

Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options when it comes to a long segment reconstruction of the bile duct. Biological scaffolds of decellularized biliary origin may offer an approach to support the replace of bile ducts. Our objective was to obtain an extracellular matrix scaffold derived from porcine extrahepatic bile ducts (dECM-BD) and to analyze its biological and biochemical properties. The efficiency of the tailored perfusion decellularization process was assessed through histology stainings. Results from 4’-6-diamidino-2-phenylindole (DAPI), Hematoxylin and Eosin (H&E) stainings, and deoxyribonucleic acid (DNA) quantification showed proper extracellular matrix (ECM) decellularization with an effectiveness of 98%. Immunohistochemistry results indicate an effective decrease in immunogenic marker as human leukocyte antigens (HLA-A) and Cytokeratin 7 (CK7) proteins. The ECM of the bile duct was preserved according to Masson and Herovici stainings. Data derived from scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) showed the preservation of the dECM-BD hierarchical structures. Cytotoxicity of dECM-BD was null, with cells able to infiltrate the scaffold. In this work, we standardized a decellularization method that allows one to obtain a natural bile duct scaffold with hierarchical ultrastructure preservation and adequate cytocompatibility.

show abstract

“…Decellularization is defined as a process to obtain natural matrices frequently used as scaffolds to replace tissues and organs. This process implies the removal of the allogeneic or xenogeneic antigens from cells in the source tissue or organ that lead to an immune response in the host body (59,60). Thus, decellularization produces a natural three-dimensional scaffold containing the main elements of the ECM, which can be repopulated with host cells (61).…”

Section: Decellularized Cell Scaffolds Containing Elastinmentioning

confidence: 99%

“…On the other hand, this process has some unsolved challenges, such as the hazard of incomplete decellularization or the uncontrollable variability in the attributes of the final matrix (59). Thus, decellularization is performed with a wide variety of intricated protocols, using diverse decellularization agents (physical, chemical, and biological) such as detergents (e.g., Sodium deoxycholate, SDS) and enzymes (e.g., trypsin) (63).…”

Section: Decellularized Cell Scaffolds Containing Elastinmentioning

confidence: 99%

Recent Advances in Elastin-Based Biomaterials

et al. 2020

View full text Add to dashboard Cite

Elastin is one of the main components of the extracellular matrix; it provides resistance and elasticity to a variety of tissues and organs of the human body, besides participating in cellular signaling. On the other hand, elastin-derived peptides are synthetic biopolymers with a similar conformation and structure to elastin, but these possess the advantage of solubility in aqueous mediums. Due to their biological activities and physicochemical properties, elastin and related peptides may be applied as biomaterials to develop diverse biomedical devices, including scaffolds, hydrogels, and drug delivery systems for tissue engineering. Likewise, the combination of elastin with natural or synthetic polymers has demonstrated to improve the mechanical properties of biomedical products and drug delivery systems. Here we comprehensively describe the physicochemical properties and physiological functions of elastin. Moreover, we offer an overview of the use of elastin and its derivative polymers as biomaterials to develop scaffolds and hydrogels for tissue engineering. Finally, we discuss some perspectives on the employment of these biopolymers to fabricate new biomedical products.

show abstract

Decellularized Scaffolds

Cited by 11 publications

References 224 publications

Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells

Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells

Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility

Recent Advances in Elastin-Based Biomaterials

Contact Info

Product

Resources

About