Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration

Hu, Lei; Gao, Zhenhua; Xu, Junji; Zhu, Zhenxin; Fan, Zhipeng; Zhang, Chunmei; Wang, Jinsong; Wang, Songlin

doi:10.1155/2017/9342714

Cited by 56 publications

(53 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tooth slices were prepared and transplanted as previous study 21 . Eighteen human impacted third molars (wisdom teeth) from eighteen healthy individuals (20‐30 years old) were received from Beijing Stomatological Hospital, Capital Medical University, followed approved guidelines and informed consent.…”

Section: Methodsmentioning

confidence: 99%

IGFBP5 enhances the dentinogenesis potential of dental pulp stem cells via JNK and ErK signalling pathways

Hao

Yang

Cao

et al. 2020

J of Oral Rehabilitation

Self Cite

View full text Add to dashboard Cite

Background: Dental stem cell transplantation has become a new method for tooth tissue regeneration. However, its molecular mechanism of the dentinogenic differentiation is still unclear, limited its application. Our previous studies found that insulin-like growth factor-binding protein 5 (IGFBP5) can promote the osteogenic differentiation of periodontal ligament stem cells and the regeneration of periodontal tissues. This study aims to clarify the effect and mechanism of IGFBP5 on the dentinogenesis of dental pulp stem cells (DPSCs). Objective and Methods: Lentiviral IGFBP5 shRNA was used to knock-down of IGFBP5. And recombinant human IGFBP5 protein (rhIGFBP5) was used to treat DPSCs. Alkaline phosphatase (ALP) staining, Alizarin red staining, quantitative calcium analysis, real-time RT-PCR and Western Blot were used to detect dentinogenic differentiation markers and related signalling pathways. Transplantation in nude mice was used to detect the dentin regeneration in vivo. Results: Depletion of IGFBP5 inhibited ALP activity and the mineralisation and reduced the expressions of osteo/dentinogenic differentiation markers BSP, DMP-1 and DSPP in DPSCs. 0.05 ng/mL rhIGFBP5 promoted ALP activity, the mineralisation and the expressions of BSP, DMP-1 and DSPP in DPSCs. In addition, 0.05 ng/mL rhIGFBP5 could promote DPSC-mediated dentin-like tissues formation in vivo. Western blot results showed that IGFBP5 activated JNK and Erk signalling pathways in DPSCs. Furthermore, inhibition of JNK pathway by SP600125, the expression of p-JNK and pErk was reduced, while inhibition of Erk pathway by PD98059, only pErk expression was decreased. Conclusions: Our results demonstrated that IGFBP5 could promote the dentinogenic differentiation and dentinogenesis potential of DPSCs via JNK and ErK signalling pathways.

show abstract

Section: Methodsmentioning

confidence: 99%

IGFBP5 enhances the dentinogenesis potential of dental pulp stem cells via JNK and ErK signalling pathways

Hao

Yang

Cao

et al. 2020

J of Oral Rehabilitation

Self Cite

View full text Add to dashboard Cite

show abstract

“…Transplantation of human DPSCs with decellularized dental pulp into nude mice demonstrated ECM preservation and a pulp-like tissue structure, as revealed by histological analysis. 24 Decellularized natural porcine tooth bud has also been shown to be useful as a bioengineered scaffold for tooth regeneration, when transplanted with porcine dental epithelial cells, human dental pulp cells, and human umbilical vein endothelial cells. The implantation of samples into the mandibles of mini-pigs revealed dentin-and enamel-like tissues.…”

Section: Scaffoldsmentioning

confidence: 99%

“…Several kinds of material for scaffolds that influence DPSC properties have been reported. [19][20][21][22][23][24][25][26] Mitochondria are cytoplasmic organelles that have critical functions in energy metabolism for the regulation of stem cells. Considering analysis of the energy metabolism of stem cells for regenerative research, knowledge of mitochondrial properties is important as it should deepen our understanding of the differentiation of these cells.…”

Section: Introductionmentioning

confidence: 99%

<p>Dental Pulp Stem Cells: Advances to Applications</p>

Tsutsui¹

2020

SCCAA

105

View full text Add to dashboard Cite

Dental pulp stem cells (DPSCs) have a high capacity for differentiation and the ability to regenerate a dentin/pulp-like complex. Numerous studies have provided evidence of DPSCs' differentiation capacity, such as in neurogenesis, adipogenesis, osteogenesis, chondrogenesis, angiogenesis, and dentinogenesis. The molecular mechanisms and functions of DPSCs' differentiation process are affected by growth factors and scaffolds. For example, growth factors such as basic fibroblast growth factor (bFGF), transforming growth factor-β (TGF-β), nerve growth factor (NGF), platelet-derived growth factor (PDGF), and bone morphogenic proteins (BMPs) influence DPSC fate, including in differentiation, cell proliferation, and wound healing. In addition, several types of scaffolds, such as collagen, hydrogel, decellularized bioscaffold, and nanofibrous spongy microspheres, have been used to characterize DPSC cellular attachment, migration, proliferation, differentiation, and functions. An appropriate combination of growth factors and scaffolds can enhance the differentiation capacity of DPSCs, in terms of optimizing not only dental-related expression but also dental pulp morphology. For a cell-based clinical approach, focus has been placed on the tissue engineering triad [cells/bioactive molecules (growth factors)/scaffolds] to characterize DPSCs. It is clear that a deep understanding of the mechanisms of stem cells, including their aging, self-renewal, microenvironmental homeostasis, and differentiation correlated with cell activity, the energy for which is provided from mitochondria, should provide new approaches for DPSC research and therapeutics. Mitochondrial functions and dynamics are related to the direction of stem cell differentiation, including glycolysis, oxidative phosphorylation, mitochondrial metabolism, mitochondrial transcription factor A (TFAM), mitochondrial elongation, and mitochondrial fusion and fission proteins. This review summarizes the effects of major growth factors and scaffolds for regenerating dentin/pulp-like complexes, as well as elucidating mitochondrial properties of DPSCs for the development of advanced applications research.

show abstract

“…In addition, 3D nano-fibrous gelatin/silica bioactive glass hybrid scaffolds provide a suitable microenvironment that mimics the architecture and composition of a natural dental micromilieu and enhances the growth and differentiation of human DSCs[ 107 ]. Furthermore, the administration of human DSCs associated with acellular dental pulp resulted in pulp-like tissue structures and the maintenance of ECM[ 108 ]. DSCs seeded in 3D scaffold-free stem-cell sheet-derived pellets promote odontogenic differentiation[ 109 ].…”

Section: Tooth Reconstructionmentioning

confidence: 99%

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Granz

Gorji

2020

WJSC

View full text Add to dashboard Cite

Dental stem cells (DSCs) are self-renewable cells that can be obtained easily from dental tissues, and are a desirable source of autologous stem cells. The use of DSCs for stem cell transplantation therapeutic approaches is attractive due to their simple isolation, high plasticity, immunomodulatory properties, and multipotential abilities. Using appropriate scaffolds loaded with favorable biomolecules, such as growth factors, and cytokines, can improve the proliferation, differentiation, migration, and functional capacity of DSCs and can optimize the cellular morphology to build tissue constructs for specific purposes. An enormous variety of scaffolds have been used for tissue engineering with DSCs. Of these, the scaffolds that particularly mimic tissue-specific micromilieu and loaded with biomolecules favorably regulate angiogenesis, cell-matrix interactions, degradation of extracellular matrix, organized matrix formation, and the mineralization abilities of DSCs in both in vitro and in vivo conditions. DSCs represent a promising cell source for tissue engineering, especially for tooth, bone, and neural tissue restoration. The purpose of the present review is to summarize the current developments in the major scaffolding approaches as crucial guidelines for tissue engineering using DSCs and compare their effects in tissue and organ regeneration.

show abstract

Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration

Cited by 56 publications

References 41 publications

IGFBP5 enhances the dentinogenesis potential of dental pulp stem cells via JNK and ErK signalling pathways

IGFBP5 enhances the dentinogenesis potential of dental pulp stem cells via JNK and ErK signalling pathways

<p>Dental Pulp Stem Cells: Advances to Applications</p>

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Contact Info

Product

Resources

About