Deciphering extracellular antibiotic resistance genes (eARGs) in activated sludge by metagenome

Zhou, Shuai; Zhu, Yijing; Yuan, Yan; Wang, Weigang

doi:10.1016/j.watres.2019.06.048

Cited by 110 publications

(33 citation statements)

References 68 publications

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“…In fact, several studies suggest that eARGs can persist in wastewater effluents even after several advanced treatment technologies, including: membrane filtration, chlorine disinfection and ultraviolet light disinfection. Consequently, the released eARGs into the aquatic environment may contribute to the sources of antibiotic resistance both in environmental and pathogenic bacteria [ 40 , 48 , 49 , 50 ].…”

Section: Edna Persistence and Natural Transformationmentioning

confidence: 99%

“…Zhou et al, (2019) investigated the abundance and occurrence of eARGs in activated sludge waste from five wastewater treatment plants [ 50 ]. Although there was a major difference observed in the distribution pattern of species abundance between municipal WWTPs (n = 4) and swine WWTP (n = 1), all municipal WWTPs had shown no significant difference.…”

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 99%

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Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Sivalingam

Poté

Prabakar³

2020

Pathogens

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Over the past decades, the rising antibiotic resistance bacteria (ARB) are continuing to emerge as a global threat due to potential public health risk. Rapidly evolving antibiotic resistance and its persistence in the environment, have underpinned the need for more studies to identify the possible sources and limit the spread. In this context, not commonly studied and a neglected genetic material called extracellular DNA (eDNA) is gaining increased attention as it can be one of the significant drivers for transmission of extracellular ARGS (eARGs) via horizontal gene transfer (HGT) to competent environmental bacteria and diverse sources of antibiotic-resistance genes (ARGs) in the environment. Consequently, this review highlights the studies that address the environmental occurrence of eDNA and encoding eARGs and its impact on the environmental resistome. In this review, we also brief the recent dedicated technological advancements that are accelerating extraction of eDNA and the efficiency of treatment technologies in reducing eDNA that focuses on environmental antibiotic resistance and potential ecological health risk.

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Section: Edna Persistence and Natural Transformationmentioning

confidence: 99%

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 99%

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Sivalingam

Poté

Prabakar³

2020

Pathogens

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“…Furthermore, adsorbed DNA is transformed at higher rates than free DNA, making bacterial aggregates a good spot for transformation due to the high amounts of extracellular material where DNA can be attached ( Dong et al., 2019 ). Several studies of exDNA in WWTPs have shown the high abundance of extracellular MGEs (65% of the exDNA) ( Calderón-Franco et al., 2020 ) and the presence of several ARGs ( Dong et al., 2019 ; Yuan et al., 2019 ; Zhou et al., 2019 ). However, nothing is currently known about the OMP-degrading genes present in exDNA in WWTPs.…”

Section: Catabolic Mobile Genetic Elements In Wastewater Treatment Plmentioning

confidence: 99%

The role of mobile genetic elements in organic micropollutant degradation during biological wastewater treatment

2020

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Wastewater treatment plants (WWTPs) are crucial for producing clean effluents from polluting sources such as hospitals, industries, and municipalities. In recent decades, many new organic compounds have ended up in surface waters in concentrations that, while very low, cause (chronic) toxicity to countless organisms. These organic micropollutants (OMPs) are usually quite recalcitrant and not sufficiently removed during wastewater treatment. Microbial degradation plays a pivotal role in OMP conversion. Microorganisms can adapt their metabolism to the use of novel molecules via mutations and rearrangements of existing genes in new clusters. Many catabolic genes have been found adjacent to mobile genetic elements (MGEs), which provide a stable scaffold to host new catabolic pathways and spread these genes in the microbial community. These mobile systems could be engineered to enhance OMP degradation in WWTPs, and this review aims to summarize and better understand the role that MGEs might play in the degradation and wastewater treatment process. Available data about the presence of catabolic MGEs in WWTPs are reviewed, and current methods used to identify and measure MGEs in environmental samples are critically evaluated. Finally, examples of how these MGEs could be used to improve micropollutant degradation in WWTPs are outlined. In the near future, advances in the use of MGEs will hopefully enable us to apply selective augmentation strategies to improve OMP conversion in WWTPs.

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“…The genes tetA, tetM, tetW, and tetX were a large proportion of the sludge samples. (Xu et al 2020;Zhou et al 2019;Zhang et al 2019). However, in the presence of trace tetracycline, the relative abundances of efflux pumps, such as tetA and tetG, tended to remarkably increase in the UNITANK process (Liu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the elimination effectiveness of tetracycline and AmpC β-lactamase resistance genes in a municipal wastewater treatment plant using four parallel processes

et al. 2020

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Municipal wastewater treatment plants (mWWTPs), considered reservoirs of antibiotic resistance genes (ARGs), are selected to compare the contributions of technology and process to ARG removal. Fifteen ARGs (tetA, tetB, tetC, tetE, tetG, tetL, tetM, tetO, tetQ, tetS, tetX, MOX, CIT, EBC, and FOX) and two integron genes (intI1, intI2) were tracked and detected in wastewater samples from a large-scale mWWTP with four parallel processes, including three biological technologies of AAO (anaerobic-anoxic-oxic), AB (adsorption-biodegradation), and UNITANK, two different disinfection technologies, and two primary sedimentation steps. The results showed that ARGs were widely detected, among which tetA and tetM had the highest detection rate at 100%. AAO was the most effective process in removing ARGs, followed by the AB and UNITANK processes, where the separation step was critical: 37.5% AmpC β-lactamase genes were reduced by the secondary clarifier. UV disinfection was more efficient than chlorination disinfection by 47.0% in ARG removal. Both disinfection and primary sedimentation processes could effectively remove integrons, and the swirling flow grit chamber was a more effective primary settling facility in total ARG removal than the aerated grit chamber. The tet genes and AmpC β-lactamase genes were significantly correlated with the water quality indexes of BOD 5 , COD Cr , SS, TP, TOC, pH and NH 4 +-N (p < 0.05). In addition, the correlation between efflux pump genes and AmpC β-lactamase genes was strongly significant (r 2 = 0.717, p < 0.01). This study provides a more powerful guide for selecting and designing treatment processes in mWWTPs with additional consideration of ARG removal.

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Deciphering extracellular antibiotic resistance genes (eARGs) in activated sludge by metagenome

Cited by 110 publications

References 68 publications

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

The role of mobile genetic elements in organic micropollutant degradation during biological wastewater treatment

Comparison of the elimination effectiveness of tetracycline and AmpC β-lactamase resistance genes in a municipal wastewater treatment plant using four parallel processes

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