Deciphering glycan linkages involved in Jurkat cell interactions with gold-coated nanofibers via sugar-displayed thiols

Du, Junbao; Lin, Pao Hwa; Aich, Udayanath; Tan, Elaine; Kim, Hyo Jun; Sampathkumar, Srinivasa-Gopalan; Yarema, Kevin J.

doi:10.1016/j.bmcl.2011.05.044

Cited by 17 publications

(20 citation statements)

References 22 publications

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“…The past few years have witnessed significant progresses in applications of the electrospun nanomaterials, demonstrated by numbers of recent scientific publications on this area [24,44,82,[219][220][221]. NFN membranes possess almost all the applications that traditional electrospun fibrous membranes have, and due to its much smaller fiber diameter and unique net-like structure, which bring ESN NFN membranes highly attractive to some special area, such as ultra-fine filters to intercept viruses, ultrasensitive sensors, as well as biomedicine.…”

Section: Applications Of Nfn Membranes: Solving Global Issuesmentioning

confidence: 99%

Electro-spinning/netting: A strategy for the fabrication of three-dimensional polymer nano-fiber/nets

Wang

Ding

Sun

et al. 2013

Progress in Materials Science

478

300

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a b s t r a c tSince 2006, a rapid development has been achieved in a subject area, so called electro-spinning/netting (ESN), which comprises the conventional electrospinning process and a unique electro-netting process. Electronetting overcomes the bottleneck problem of electrospinning technique and provides a versatile method for generating spider-web-like nanonets with ultrafine fiber diameter less than 20 nm. Nano-nets, supported by the conventional electrospun nanofibers in the nano-fiber/nets (NFN) membranes, exhibit numerious attractive characteristics such as extremely small diameter, high porosity, and Steiner tree network geometry, which make NFN membranes optimal candidates for many significant applications. The progress made during the last few years in the field of ESN is highlighted in this review, with particular emphasis on results obtained in the author's research units. After a brief description of the development of the electrospinning and ESN techniques, several fundamental properties of NFN nanomaterials are addressed. Subsequently, the used polymers and the state-of-the-art strategies for the controllable fabrication of NFN membranes are highlighted in terms of the ESN process. Additionally, we highlight some potential applications associated with the remarkable features of NFN nanostructure. Our discussion is concluded with some personal perspectives on the future development in which this wonderful technique could be pursued.

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Section: Applications Of Nfn Membranes: Solving Global Issuesmentioning

confidence: 99%

Electro-spinning/netting: A strategy for the fabrication of three-dimensional polymer nano-fiber/nets

Wang

Ding

Sun

et al. 2013

Progress in Materials Science

478

300

View full text Add to dashboard Cite

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“…It may also promote the differentiation of stem cells.T reating non-adhesive human Tcell leukemia cells with Neu5TGc induced spontaneous cellcell clustering,e xpression of ß-catenin, and changes in cell morphology. [240][241][242] In contrast to azides and diazirines,thiols are not abiotic. However,thiols are not found in the glycocalyx of untreated cells.T hiolylated sialosides are able to form cis-disulfide linkages.F urthermore,t hey provide the capability to bind gold, which can be used to visualize sialosides in cell compartments.…”

Section: Modifications Bearing Thiolsmentioning

confidence: 99%

Metabolic Glycoengineering with N‐Acyl Side Chain Modified Mannosamines

2016

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In metabolic glycoengineering (MGE), cells or animals are treated with unnatural derivatives of monosaccharides. After entering the cytosol, these sugar analogues are metabolized and subsequently expressed on newly synthesized glycoconjugates. The feasibility of MGE was first discovered for sialylated glycans, by using N-acyl-modified mannosamines as precursor molecules for unnatural sialic acids. Prerequisite is the promiscuity of the enzymes of the Roseman-Warren biosynthetic pathway. These enzymes were shown to tolerate specific modifications of the N-acyl side chain of mannosamine analogues, for example, elongation by one or more methylene groups (aliphatic modifications) or by insertion of reactive groups (bioorthogonal modifications). Unnatural sialic acids are incorporated into glycoconjugates of cells and organs. MGE has intriguing biological consequences for treated cells (aliphatic MGE) and offers the opportunity to visualize the topography and dynamics of sialylated glycans in vitro, ex vivo, and in vivo (bioorthogonal MGE).

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“…Des Weiteren fördert es die Differenzierung von Stammzellen. Behandelt man nichtadhäsive menschliche T‐Zell‐Leukämiezellen mit Neu5TGc, führt dies zu spontanem Zell‐Zell‐Clustering, der Expression von β‐Catenin und zu Veränderungen der Zellmorphologie …”

Section: Metabolisches Glykoengineering Der N‐acyl‐seitenketten Von unclassified

Metabolisches Glykoengineering mit N‐Acyl‐Seiten‐ ketten‐modifizierten Mannosaminen

2016

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Beim metabolischen Glykoengineering (MGE) werden Zellen und Tiere mit nichtnatürlichen Derivaten von Monosacchariden behandelt. Diese werden nach ihrer Aufnahme ins Zytosol metabolisiert und anschließend auf neusynthetisierten Glykokonjugaten exprimiert. MGE wurde zuerst für sialylierte Glykane realisiert, mit N‐Acyl‐modifizierten Mannosaminen als Vorstufen für nichtnatürliche Sialinsäuren. Voraussetzung ist die Promiskuität der Enzyme des Roseman‐Warren‐Biosyntheseweges. Diese tolerieren spezifische Modifikationen der N‐Acyl‐Seitenkette von Mannosaminderivaten, z. B. Elongation mit Methylen‐Gruppen (aliphatische Modifikationen) oder Einfügen reaktiver Gruppen (bioorthogonale Modifikationen). Nichtnatürliche Sialinsäuren werden in Glykokonjugate von Zellen und Organen integriert. MGE hat faszinierende biologische Konsequenzen für die behandelten Zellen (aliphatisches MGE) und ermöglicht die Visualisierung der Topographie und Dynamik der sialylierten Glykane in vitro, ex vivo und in vivo (bioorthogonales MGE).

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Deciphering glycan linkages involved in Jurkat cell interactions with gold-coated nanofibers via sugar-displayed thiols

Cited by 17 publications

References 22 publications

Electro-spinning/netting: A strategy for the fabrication of three-dimensional polymer nano-fiber/nets

Electro-spinning/netting: A strategy for the fabrication of three-dimensional polymer nano-fiber/nets

Metabolic Glycoengineering with N‐Acyl Side Chain Modified Mannosamines

Metabolisches Glykoengineering mit N‐Acyl‐Seiten‐ ketten‐modifizierten Mannosaminen

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