Deciphering the Colonization Strategies in Roots of Long-Term Fertilized Festuca rubra

Corcoz, Larisa; Păcurar, F.; Vaida, Ioana; Pleşa, Anca; Moldovan, Cristina; Stoian, Vlad; Vidican, Roxana

doi:10.3390/agronomy12030650

Cited by 8 publications

(10 citation statements)

References 67 publications

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“…Indeed, decreases in plant root length densities are known to decrease the rate of spread of AMF, due to a lower number of plant roots available to colonise(Van Noordwijk et al, 1998). Moreover, nutrient availability has a strong in uence on the level of symbiosis with AMF, as host plants no longer invest in the fungal partner if nutrients are accessible to them in su cient quantities without the symbiosis(Corcoz et al, 2022;Nouri et al, 2014). In our study, the positive effect of trees on soil mineral N and water content would have made the GPC less dependent on symbiosis with AMF…”

mentioning

confidence: 68%

The effect of trees on arbuscular mycorrhizal fungi and nutrient acquisition of grassland plant communities in three temperate silvopastoral systems

Murray,

Avezaat,

Guilmot

et al. 2023

Preprint

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Aims Despite their importance for plant nutrient acquisition, the role of arbuscular mycorrhizal fungi (AMF) in tree-crop interactions in silvopastoral systems is unclear. The aim of this study was to investigate the effect of trees on AMF biomass and grassland root colonisation in relation with nutrient acquisition of the grassland plant community (GPC) in temperate permanent grazed silvopastures. Methods Samples were collected at two soil depths (0–20 cm and 20–60 cm) in three paired sites on commercial farms, each combining an apple (Malus domestica)-based silvopasture adjacent to a grassland managed identically excepting the presence of trees. Soil chemical and physical properties were determined. AMF biomass was measured by extracting and quantifying Neutral Lipid Fatty Acids (NLFA). GPC roots were isolated from the soil samples and their colonisation by AMF, biomass, and N and P concentrations were measured. Results Apple trees had an overall negative effect on AMF biomass, colonisation, and GPC root biomass. Trees increased soil mineral N and N concentrations in GPC roots, which were likely N-limited at all sites as indicated by N:P ratios comprised between 5 and 9. Conclusions Rather than mediating interactions between trees and the GPC, AMF colonisation and biomass likely followed direct effects of trees on nutrient cycling and consequential changes in root biomass. We speculate that trees lifted nutrient limitations in the topsoil due to ‘hydraulic lift’ and ‘nutrient pumping’ mechanisms which would have decreased root biomass by shifting the functional equilibrium of the GPC towards above-ground production.

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confidence: 68%

The effect of trees on arbuscular mycorrhizal fungi and nutrient acquisition of grassland plant communities in three temperate silvopastoral systems

Murray,

Avezaat,

Guilmot

et al. 2023

Preprint

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“…Data from each of the segments were further analyzed for the detection of a colonization strategy. This procedure followed the concept proposed by Corcoz et al [52], the four strategies considered being: resistance, proliferative, transfer, and storage. Changes to the proposed concept were performed to the intensity, arbuscules and vesicles values, which indicates the orientation of each segment toward a clear strategy.…”

Section: Discussionmentioning

confidence: 99%

“…The overall microscopic images were 15 for each of the analyzed segments and reassembled into one larger image to provide the mycorrhizal colonization base for an entire segment. Images from each segment were coded based on the indexes in the mycorrhizal pattern method [50] in order to be further converted with the MycoPatt tool [50] in colors and to provide the colonization image and patterns [51,52]. From the entire experiment resulted a database of 4500 values for each of the colonization parameters: colonization frequency (%); colonization intensity (%); arbuscules and vesicles abundance (%); the overall colonization degree (%); the share of non-mycorrhizal areas (%) in roots; the report between mycorrhized and non-mycorrhized areas and the report between arbuscules and vesicles.…”

Section: Laboratory Analysismentioning

confidence: 99%

“…This type of analysis is necessary for a deeper analysis of the colonization mechanism, which acts with various intensities within the same root. Four strategies were identified for A. capillaris, based on the proposal made by Corcoz et al [52] Intensity of colonization in root segments is set as the indicator of all the colonization strategies. Based on the intensity value and the presence/absence of arbuscules and vesicles, the strategies present a clear orientation.…”

Section: Establishment Of Multi-point Analysis As a Translation Tool ...mentioning

confidence: 99%

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Long-Term Fertilization Alters Mycorrhizal Colonization Strategy in the Roots of Agrostis capillaris

et al. 2022

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Long-term fertilization targets mycorrhizal fungi adapted to symbiotic exchange of nutrients, thus restricting their colonization potential and re-orienting the colonization strategies. The MycoPatt tool has a high applicability in quantifying the symbiotic process with the identification of mycorrhizal indices and projection of mycorrhizal patterns. Organic treatments increase the symbiotic process, visible in values of colonization frequency and intensity, with about 6% more than the native status of colonization. At the opposite pole, organic-mineral treatments decrease the colonization parameters by up to half of the organic treatment. All of the colonization parameters show significant correlations, except for the arbuscules/vesicle ratio (0.03). All the applied treatments, except for the organic one, record multiple root segments with a colonization degree lower than 10%. The application of treatments changes the strategy of native colonization from a transfer (40%) and storage (37%) to a predominant storage (50%) for organic treatment, and are mainly proliferative between 38–50% in mixed and mineral treatments. The high amount of mineral components increases also the presence of resistance conditions strategies. The use of mycorrhizal pattern maps, with the inclusion of colonization strategies, presents an important direction in understanding the evolution of mutual relations, and to explore in-depth the efficiency of the whole symbiotic process.

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“…Arbuscules and vesicles from each treatment and phenophase were both analyzed in scatterplots, produced by the “stats” package from the R platform ( RCore Team, 2021 ). Each set of data from treated and untreated variants was filtered and grouped based on intensity, arbuscules, and vesicles, in order to identify the number of clear colonization strategies ( Corcoz et al., 2022b ). Variants with an intensity lower than 10% were considered in the group of resistance colonization conditions; the intensity between 10% and 25% was assessed as proliferative colonization strategy; the roots with an intensity higher than 25% and an arbuscule/vesicle ratio higher than 1.0 were considered in the group of transfer colonization strategy; the roots with an intensity higher than 25% and an arbuscule/vesicle ratio lower than 1.0 were considered in the group of storage colonization strategy ( Corcoz et al., 2022a ).…”

Section: Methodsmentioning

confidence: 99%

Models of mycorrhizal colonization patterns and strategies induced by biostimulator treatments in Zea mays roots

et al. 2022

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Agronomic inputs and technologies, especially fertilizers, act on the evolution of the symbiotic partnership between arbuscular mycorrhizal fungi and cultivated plants. The use of the MycoPatt method for the assessment of mycorrhizas in maize roots leads to the extraction of large parameter databases with an increased resolution over the colonization mechanism. The application of a biostimulator treatment on plants acted toward a reduction of root permissiveness for mycorrhizas. The phenomenon was noticeable through an increased colonization variability that overlapped with plant nutritional needs. The annual characteristic of the plant was highlighted by the simultaneous presence of arbuscules and vesicles, with a high share of arbuscules in the advanced phenophases. Colonized root parts presented numerous arbuscule-dominated areas in all phenophases, which indicated a continuous formation of these structures and an intense nutrient transfer between partners. Mycorrhizal maps showed the slowing effect of the biostimulators on colonization, with one phenophase delay in the case of biostimulated plants compared to the ones without biostimulators. The forecast models presented gradual colonization in plants without biostimulators, with the expansion of new hyphal networks. The use of biostimulators on plants exhibited a lower permissiveness for new colonization areas, and the mechanism relies on hyphae developed in the former phenophases.

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Deciphering the Colonization Strategies in Roots of Long-Term Fertilized Festuca rubra

Cited by 8 publications

References 67 publications

The effect of trees on arbuscular mycorrhizal fungi and nutrient acquisition of grassland plant communities in three temperate silvopastoral systems

The effect of trees on arbuscular mycorrhizal fungi and nutrient acquisition of grassland plant communities in three temperate silvopastoral systems

Long-Term Fertilization Alters Mycorrhizal Colonization Strategy in the Roots of Agrostis capillaris

Models of mycorrhizal colonization patterns and strategies induced by biostimulator treatments in Zea mays roots

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