Deciphering the contribution of PerR to oxidative stress defense system in Clostridium tyrobutyricum

Abstract

As a next‐generation probiotic, Clostridium tyrobutyricum, which is considered obligate anaerobe, has been extensively considered a promising candidate for future use to promote health benefits. The industrial applications were limited by its weak tolerance to oxygen stress during fermentation. Here, we enhanced the oxidative stress tolerance of C. tyrobutyricum L319 by deleting the peroxide response regulator gene perR using an endogenous CRISPR‐Cas system. The mutant strain exhibited greatly improved growth when exposed to oxygen, which also showed a shortened lag phase compared with the control strain. Consistently, a concomitant increase in butyric acid productivity from 0.08 to 0.13 g/L/h in the presence of oxygen was observed. Decreased intracellular ROS level sand NAD+/NADH ratios were also observed in the mutant strain. Furthermore, transcriptome and binding target analysis revealed that genes encoding transcriptional regulators and proteins related to redox balance were upregulated. Changes in carbohydrate, amino acid and purine metabolisms upon oxygen exposure were also observed. Importantly, phylogenomic analysis indicated that PerR may be a universal engineering target in diverse Clostridium species. These findings will contribute to the development of robust C. tyrobutyricum strains for efficient butyric acid production in the food industry.

Metabolic and Bioprocess Engineering of Clostridium tyrobutyricum for Butyl Butyrate Production on Xylose and Shrimp Shell Waste

“…Upon being constructed, the recombinant plasmids were conjugated into C. tyrobutyricum L319 cells via the E. coli CA434 strain [28]. Colony PCR was used to confirm the successful transformation.…”

Comparative transcriptome analysis reveals the contribution of amino acid transporters to acid tolerance in Lactococcus lactis

“…Acidity is one of the most prominent stress factors, especially when selecting potential probiotics (Qi et al., 2021; Yang et al., 2023). The accumulation of acidic fermentative products such as lactate and corresponding drop in pH can lead to cell growth inhibition and even cell death due to the entrance of undissociated organic acids into the cytoplasm through simple diffusion (Zhu et al., 2022).…”

Clostridium tyrobutyricum in Combination with Chito-oligosaccharides Modulate Inflammation and Gut Microbiota for Inflammatory Bowel Disease Treatment

“…The emerging focus on gut-derived Clostridium species stems from their capacity to produce SCFAs effectively . Among these, Clostridium tyrobutyricum, a Gram-positive anaerobic bacterium known for its butyrate synthesis, shows promise in the management and prevention of various human and animal ailments. − Therefore, C. tyrobutyricum could be explored as a potential biotherapeutic for IBD treatment.…”