Deciphering the Protective Role of Nitric Oxide against Salt Stress at the Physiological and Proteomic Levels in Maize

Bai, Xuegui; Yang, Liming; Yang, Yunqiang; Ahmad, Parvaiz; Yang, Yongping; Hu, Xiangyang

doi:10.1021/pr200333f

Cited by 102 publications

(84 citation statements)

References 41 publications

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“…Discussion DETA/NO was successfully used to elevate the endogenous NO level and reduce salt-induced damage in maize (Keyster et al, 2012). This effect could be reversed by NO metabolic scavengers and inhibitors (Bai et al, 2011). In the present study, the protective role of NO against salt stress was confirmed in maize using DETA/NO and by inhibiting its synthesis with l-NNA.…”

Section: Gene Expression Studiessupporting

confidence: 64%

“…NO enhanced salt tolerance in maize due to the activation of proton pumps and the Na + /H + antiport, as indicated by improved growth, increased dry matter accumulation and greater chlorophyll content (Zhang et al, 2006). The protective https://repository.uwc.ac.za/ effect of NO against salt stress in maize can also be linked to the enhanced activity of antioxidant enzymes (Bai et al, 2011;Keyster et al, 2012). The induction of various antioxidant enzymes (catalase, APX, SOD) by NO was also observed under salt stress in chickpea (Sheokand et al, 2008), wheat (Zheng et al, 2009) and rice (Uchida et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Nitric oxide affects salt-induced changes in free amino acid levels in maize

Boldizsár¹,

Simon‐Sarkadi²,

Szirtes³

et al. 2013

Journal of Plant Physiology

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Abstract:It was assumed that salt-induced redox changes affect amino acid metabolism in maize (Zea mays L.), and this influence may be modified by NO. The applied NaCl treatment reduced the fresh weight of shoots and roots. This decrease was smaller after the combined application of NaCl and an NO-donorETA/NO) in the shoots, while it was greater after simultaneous treatment with NaCl and nitro-l-arginine (l-NNA, inhibitor of NO synthesis) in the roots. The quantum yield efficiency of photosystem II was not influenced by the treatments. NaCl had a significant effect on the redox environment in the leaves as it was shown by the increase in the amount of glutathione disulphide and in the redox potential of the glutathione/glutathione disulphide redox pair. This influence of NaCl was modified by DETA/NO and l-NNA. Pharmacological modification of NO levels affected salt-induced changes in both the total free amino acid content and in the free amino acid composition. NaCl alone increased the concentration of almost all amino acids which effect was strengthened by DETA/NO in the case of Pro. l-NNA treatment resulted in a significant increase in the Ala, Val, Gly and Tyr contents. The Ile, Lys and Val concentrations rose considerably after the combined application of NaCl and DETA/NO compared to NaCl treatment alone in the recovery phase. NaCl also increased the expression of several genes related to the amino acid and antioxidant metabolism, and this effect was modified by DETA/NO. In conclusion, modification of NO levels affected salt-induced, glutathione-dependent redox changes and simultaneously the free amino acid composition and the level of several free amino acids. The observed much higher Pro content in plants treated with both NaCl and DETA/NO during recovery may contribute to the protective effect of NO against salt stress.

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Section: Gene Expression Studiessupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Boldizsár¹,

Simon‐Sarkadi²,

Szirtes³

et al. 2013

Journal of Plant Physiology

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“…NO production is altered when plants are subjected to abiotic or biotic stresses (13,14). High salt, a major environmental factor that limits agriculture yield, induces a quick endogenous NO accumulation in plants (15,16), and triggers enhanced Na + influx and reduced K + absorption in the root (17). Both endogenously produced NO and exogenously applied NO have been proposed to enhance plant salt tolerance (18-21) by attenuating high saltinduced increases in the Na + to K + ratio.…”

mentioning

confidence: 99%

Nitric oxide negatively regulates AKT1-mediated potassium uptake through modulating vitamin B6 homeostasis in Arabidopsis

Xia

Kong

Xue

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Nitric oxide (NO), an active signaling molecule in plants, is involved in numerous physiological processes and adaptive responses to environmental stresses. Under high-salt conditions, plants accumulate NO quickly, and reorganize Na + and K + contents. However, the molecular connection between NO and ion homeostasis is largely unknown. Here, we report that NO lowers K + channel AKT1-mediated plant K + uptake by modulating vitamin B6 biosynthesis. In a screen for Arabidopsis NO-hypersensitive mutants, we isolated sno1 (sensitive to nitric oxide 1), which is allelic to the previously noted mutant sos4 (salt overly sensitive 4) that has impaired Na + and K + contents and overproduces pyridoxal 5′-phosphate (PLP), an active form of vitamin B6. We showed that NO increased PLP and decreased K + levels in plant. NO induced SNO1 gene expression and enzyme activity, indicating that NO-triggered PLP accumulation mainly occurs through SNO1-mediated vitamin B6 salvage biosynthetic pathway. Furthermore, we demonstrated that PLP significantly repressed the activity of K + channel AKT1 in the Xenopus oocyte system and Arabidopsis root protoplasts. Together, our results suggest that NO decreases K + absorption by promoting the synthesis of vitamin B6 PLP, which further represses the activity of K + channel AKT1 in Arabidopsis. These findings reveal a previously unidentified pivotal role of NO in modulating the homeostasis of vitamin B6 and potassium nutrition in plants, and shed light on the mechanism of NO in plant acclimation to environmental changes.genetic approach | electrophysiological studies | potassium nutrition N itric oxide (NO) acts as a crucial signaling molecule in various physiological processes in plants, such as seed germination and dormancy (1, 2), root development (3), leaf senescence (4, 5), floral transition (6), stomatal movement (7, 8), iron homeostasis (9, 10), and hormone responses (11,12). NO production is altered when plants are subjected to abiotic or biotic stresses (13,14). High salt, a major environmental factor that limits agriculture yield, induces a quick endogenous NO accumulation in plants (15,16), and triggers enhanced Na + influx and reduced K + absorption in the root (17). Both endogenously produced NO and exogenously applied NO have been proposed to enhance plant salt tolerance (18-21) by attenuating high saltinduced increases in the Na + to K + ratio. Genetic analysis showed that K + nutrition, but not Na + , plays critical role in plant salt tolerance (22). However, the molecular basis of NO effect on K + or Na + content is elusive.K + levels in plant tissues are determined by K + uptake and translocation, which are mediated by a large number of transporters and channels. In Arabidopsis, the K + channel AKT1 (23, 24) and K + transporter AtHAK5 (25) are the two major molecular entities responsible for K + absorption from the environment (26-28). AKT1 contributes to K + acquisition over a wide range of external K + concentrations (10 μM-10 mM), whereas AtHAK5 mediates limited uptake capac...

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“…Arfan (2009) reported that, SAinduced SOD activity accompanied with an increase in shoot Ca 2 + supported the view that if sufficient Ca 2 + is present, it acts as a second messenger and causes a transient increase in H 2 O 2 , which in turn induces antioxidant enzymes leading to decrease in ROS on long-term basis. It has also been reported that NO treatment further improved protein accumulations and enzyme activities including APX, Mn-SOD, GR, and growth via G proteins (Bai et al 2011).…”

Section: Discussionmentioning

confidence: 89%

“…Therefore, an efficient H 2 O 2 scavenging system is Simaei et al 2011). Bai et al (2011) found that NO affected a series of protein accumulation involving in material and energy metabolism, including ATPase and LOX.…”

Section: Discussionmentioning

confidence: 99%

Salicylic acid and nitric oxide alleviate osmotic stress in wheat (Triticum aestivum L.) seedlings

Alavi

Arvin

Kalantari

2014

Journal of Plant Interactions

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Salicylic acid (SA) and nitric oxide (NO) are reported to alleviate the damaging effects of stress in plants rather similarly when applied at appropriate low concentrations. An experiment was therefore conducted to study the impact of SA, sodium nitroprusside (SNP; as NO donor), and methylene blue (MB; as a guanylate cyclase inhibitor) on wheat seedling performance under osmotic stress. Osmotic stress significantly reduced shoot fresh weight (SFW), chlorophyll contents (Chla, Chlb, total Chl), and membrane stability index (MSI) and also increased malondialdehyde (MDA) level, lipoxygenase (LOX) activity, and hydrogen peroxide production. Moreover, enzymatic antioxidant activities including superoxide dismutase, guaiacol peroxidase, and glutathione reductase activity were enhanced under osmotic stress. On the contrary, SA or SNP pretreatment reduced the damaging effects of osmotic stress by further enhancing the antioxidant activities that led to increased SFW, Chl, and MSI and reduced MDA level and LOX activity. However, pretreatment of plants with MB reversed or reduced the protective effects of SA and SNP suggesting that the protective effects were likely attributed to NO signaling. Therefore, NO may act as downstream of SA signaling in reduction of induced oxidative damage in wheat seedlings.

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Deciphering the Protective Role of Nitric Oxide against Salt Stress at the Physiological and Proteomic Levels in Maize

Cited by 102 publications

References 41 publications

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Nitric oxide negatively regulates AKT1-mediated potassium uptake through modulating vitamin B6 homeostasis in Arabidopsis

Salicylic acid and nitric oxide alleviate osmotic stress in wheat (Triticum aestivum L.) seedlings

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