Seventy-two spayed crossbred ewes were used in a series of five successive tests, at intervals of 15 days, in a study of the actions and interactions of androgen, oestrogen and progesterone on behavioural and vaginal oestrous responses.Androgen and oestrogen were additive in their action (128 vs. 62 services; P<0\m=.\001) on behavioural response, but androgen almost completely inhibited the expected vaginal response to oestrogen.There was a significant linear decline in behavioural sensitivity from test to test; in the five successive tests, forty-eight, forty-three, thirtyseven, thirty-two and thirty ewes were served (P<0\m=.\001). This refractory condition was partly offset by progesterone pretreatment (P<0\m=.\001), but oestrogen was necessary to maintain a constant state of psychic reactivity to androgen (P<0\m=.\001).A highly significant linear dose-response relationship was demonstrated with both testosterone and testosterone propionate (P<0\m=.\001).