Decline in rates of clearance of IgG-sensitized erythrocytes with increasing age

Melez, KA; Fries, L F; Bender, BS; Tc, Quinn; Meyer, Frank

doi:10.1182/blood.v71.6.1726.1726

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…However, complete heteropolymers, containing MAb to CR1 cross-linked with either human or mouse IgG, bind to primate erythrocytes and are cleared (26). Numerous in vivo studies indicate that IgG that is bound to erythrocytes via cell surface antigens causes the erythrocyte to be recognized and cleared by the RES (45,46), yet IgG that is bound to erythrocyte CR1 as part of a C3b-opsonized IC does not sensitize erythrocytes for clearance, but IC are cleared and removed from erythrocytes in the liver and spleen (19,20). In vitro experiments suggest that recognition of human IgG bound to primate erythrocytes via most cell surface antigens appears to be quite different from recognition of human IgG bound to erythrocyte CR1 in a C3b-opsonized IC (47).…”

Section: Discussionmentioning

confidence: 99%

Antigen‐based heteropolymers: a potential therapy for binding and clearing autoantibodies via erythrocyte cr1

Ferguson

Reist

Martin

et al. 1995

Arthritis & Rheumatism

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Objective. To determine if complexes containing monoclonal antibodies to CR1 cross-linked with antigen (antigen-based heteropolymers [AHP]) can bind the corresponding autoantibody to primate erythrocyte CR1 and promote autoantibody clearance from the circulation.Methods. AHP were constructed by cross-linking double-stranded DNA (dsDNA) to monoclonal antibodies to CR1. The ability of AHP to facilitate binding of human anti-dsDNA antibodies to primate erythrocytes was studied in vitro using a variety of radioimmunoassays (including Farr assays), enzyme immunoassays, and fluorescence-activated cell sorting. In addition, we used a monkey model to study in vivo the AHP-mediated clearance of passively infused human anti-dsDNA an ti bodies.Results. Large amounts of lupus IgG anti-dsDNA antibodies can be specifically bound to human erythrocytes via the complexes, and studies in 2 rhesus monkeys indicate that the erythrocyte-bound antibodies are rapidly cleared from the circulation.Conclusion. This methodology may allow for development of a new therapy to facilitate autoantibody clearance in autoimmune disease.Antinuclear antibodies have been recognized as a serologic hallmark of systemic lupus erythematosus

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Section: Discussionmentioning

confidence: 99%

Antigen‐based heteropolymers: a potential therapy for binding and clearing autoantibodies via erythrocyte cr1

Ferguson

Reist

Martin

et al. 1995

Arthritis & Rheumatism

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“…The effect of age on phagocytes has previously been studied using a variety of methods (14,(39)(40)(41). Receptor number has been reported unaffected with age (42).…”

Section: F E M a L E M A L Ementioning

confidence: 99%

“…Macrophages show little dysfunction with age (44), and their function appears to be maintained in well-studied animal models, as well as in vitro (45,46). However, Melez et al (41) reported an age-related decrease in Fc-mediated clearance rates of IgG sensitized erythrocytes by splenic macrophages. Nagel et al (47) found the ability of PMNL to reduce nitroblue tetrazolium and to kill Staphylococcus aureus to be independent of age, but superoxide production by PMNL in response to latex particles was significantly lower in elderly adults.…”

Section: F E M a L E M A L Ementioning

confidence: 99%

The influence of age and sex on phagocyte chemiluminescence

McCafferty

Cree

McMurdo

1995

J. Biolumin. Chemilumin.

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The process of ageing is associated with increased susceptibility to infection. Phagocytes form the primary defence mechanism against infecting microorganisms, but the influence of ageing on phagocyte function remains controversial. In this study we have applied a microtitre plate phagocyte chemiluminescence (CL) assay suitable for clinical use to compare phagocyte oxidative metabolism in younger healthy subjects (age 20-60 years) and healthy older (60-70 years) subjects. Polymorphonuclear leukocytes (PMNL) and monocytes were stimulated using phorbol myristate acetate (PMA), serum opsonized zymosan (SOZ), and non-opsonized zymosan (ZYM) in the presence of both lucigenin and luminol. Monocytes showed a higher luminol-enhanced CL response to PMA in males compared with females in the younger age group. No PMNL differences were observed between the sexes. Although no differences were found in relation to age when cells were stimulated with PMA and SOZ, significantly lower background (unstimulated) CL was obtained from PMNL with luminol. PMNL luminol-enhanced CL responses were also lower in response to ZYM. The findings suggest a reduced response of PMNL from older subjects to minimal stimulation. This could be related to abnormalities in the triggering of the respiratory burst or myeloperoxidase release due to ageing. The influence of age and sex should be taken into account in clinical studies of phagocyte CL.

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“…However, the effects of aging on macrophages, which participate in a variety of host defence mechanisms, are quite contradictory. There are reports that macrophage-dependent host defence mechanisms are decreased [4][5][6], apparently unaffected [7] or even enhanced [8] with age.…”

Section: Introductionmentioning

confidence: 99%

Decrease in free-radical production with age in rat peritoneal macrophages

Alvarez

Conde

Machado

et al. 1995

Biochemical Journal

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The respiratory-burst reaction has been studied in rat peritoneal macrophages of different ages (3, 12 and 24 months) using phorbol 12-myristate 13-acetate (PMA) to stimulate NADPH oxidase. Production of O2-. and H2O2 decreased with age (about 50 and 75% respectively); however, no difference in NADPH oxidase activity was found. NO. production was also reduced with age (40%). Furthermore, a progressive and significant decrease in the pentose phosphate flux was detected as a function of age in control and PMA-stimulated macrophages. The NADPH/NADP+ ratio decreased with age in control and PMA-stimulated macrophages. Glucose uptake was lower in middle-aged (12 months) and old (24 months) animals but no differences were found between these groups.

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Decline in rates of clearance of IgG-sensitized erythrocytes with increasing age

Cited by 8 publications

References 18 publications

Antigen‐based heteropolymers: a potential therapy for binding and clearing autoantibodies via erythrocyte cr1

Antigen‐based heteropolymers: a potential therapy for binding and clearing autoantibodies via erythrocyte cr1

The influence of age and sex on phagocyte chemiluminescence

Decrease in free-radical production with age in rat peritoneal macrophages

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