Decolorization and biodegradation of Indigo carmine by a textile soil isolate Paenibacillus larvae

Ramya, Mohandass; Anusha, B.; Kalavathy, S.

doi:10.1007/s10532-007-9134-6

Cited by 51 publications

(39 citation statements)

References 17 publications

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“…In order to uncover the possible mechanism of indigo dye decolorization and to identify the metabolites generated from indigo dye after bacterial treatment, various analy tical techniques were used. The thin layer chromatography (TLC) (Campos et al, 2001) and nuclear magnetic resonance spectroscopy (NMR) (Ramya et al, 2008) have been proved to be ideal methods with the quantification of secondary metabolites from treated indigo dye. Biofilm developed on polyurethane foam was taken and air dried and SEM analysis was done.…”

Section: Methodsmentioning

confidence: 99%

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Biodecolorization and Bioremediation of Denim Industrial Wastewater by Adapted Bacterial Consortium Immobilized on Inert Polyurethane Foam (PUF) Matrix: A First Approach with Biobarrier Model

Rajendran¹,

Prabhavathi²,

Karthiksundaram³

et al. 2015

Pol J Microbiol

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A b s t r a c tThe present experiments were studied on bioremediation of denim industry wastewater by using polyurethane foam (PU foam) immobilized bacterial cells. About 30 indigenous adapted bacterial strains were isolated from denim textile effluent out of which only four isolates were found to be efficient against crude indigo carmine degradation using broth decolorization method. The selected bacterial strains were identified as Actinomyces sp., (PK07), Pseudomonas sp., (PK18), Stenotrophomonas sp., (PK23) and Staphylococcus sp., (PK28) based on microscopic and biochemical characteristics. The bacterial immobilized cells have the highest number of viable cells (PK07, PK18, PK23 and PK28 appeared to be 1 × 10 8 , 1 × 10 9 , 1 × 10 6 and 1 × 10 7 CFU/ml respectively) and maximum attachment efficiency of 92% on PU foam. The complete degradation using a consortium of PU foam immobilized cells was achieved at pH 6, 27°C, 100% of substrate concentration and allowed to develop biofilm for one day (1.5% W/V). In SEM analysis, it was found that immobilization of bacterial cells using PUF stably maintained the production of various extracellular enzymes at levels higher than achieved with suspended forms. Finally, isatin and anthranilic acid were found to be degradation products by NMR and TLC. The decolorized dye was not toxic to monkey kidney cell (HBL 100) at a concentration of 50 µl and 95% of cell viability was retained. A mathematical model that describes bacterial transport with biodegradation involves a set of coupled reaction equations with non-standard numerical approach based on the time step scheme. K e y w o r d s: biobarrier model, bioflim, bioremediation, cytotoxicity test, polyurethane foam, secondary metabolites

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Section: Methodsmentioning

confidence: 99%

“…On the other hand, the Indigo dye which has undergone effective degradation was recorded by spectrum. The end product isatin exhibited three singlets at 7.5 δ, 6.9 δ and 6.8 δ which reveal the presence of the proton of SO 3 H group an aromatic proton (Ramya et al, 2008).…”

Section: Biodecolorization and Degradation Of Indigo Dye Using Varioumentioning

confidence: 97%

Biodecolorization and Bioremediation of Denim Industrial Wastewater by Adapted Bacterial Consortium Immobilized on Inert Polyurethane Foam (PUF) Matrix: A First Approach with Biobarrier Model

Rajendran¹,

Prabhavathi²,

Karthiksundaram³

et al. 2015

Pol J Microbiol

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“…where I i and I f are initial and final absorbance of the dye solution [15]. Each decolorization value is a mean of two parallel experiments.…”

Section: Decolorization Assaymentioning

confidence: 99%

“…FJ386541) and B. subtilis (GenBank accession no. FJ386544), was developed from the wastewater of a dyeing industrial unit as these microbes are naturally adapted to survive in the presence of toxic dyes(6,15). The individual isolates showed only 75%Fig.…”

mentioning

confidence: 99%

Decolorization of Direct Red 28 by mixed bacterial culture in an up-flow immobilized bioreactor

Tony

Goyal

Khanna

2009

J Ind Microbiol Biotechnol

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Aerobic mixed bacterial culture comprised of five isolates (Bacillus vallismortis, B. pumilus, B. cereus, B. subtilis and B. megaterium) identified by 16srDNA analysis was developed from wastewater samples from the aeration tank of an effluent treatment plant of a textile and dyeing industry and evaluated for its ability to decolorize azo dye Direct Red 28 in an up-flow immobilized packed bed bioreactor using marble chips as support matrix. The bioreactor was operated under two parameters: an aeration rate of 0.4 and 0.6 mmol/min at a flow rate of 60, 90 and 120 ml/h, respectively. At a constant aeration rate of 0.4 mmol/min and with flow rates of 60, 90 and 120 ml/h, optimum decolorization of 91, 75 and 72% was observed, while at an aeration rate of 0.6 mmol/min and flow rates of 60, 90 and 120 ml/h, optimum decolorization of 93, 78 and 72% was observed over 10 days. The study concluded that across the two aeration rates and the respective flow rates, the higher aeration rate of 0.6 mmol/min along with a flow rate of 60 ml/h was best suited to decolorize Direct Red 28 in the packed bed bioreactor. Spectral changes of the input and output of the bioreactor by UV-visible spectroscopy indicated decolorization of the dye solution by degradation in addition to the visual observation of the biosorption process.

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“…Além disso, os microrganismos podem não degradar totalmente o composto e transformá-lo em produtos mais tóxicos e/ou mutagênicos (Ramya et al, 2008, Robinson et al, 2001.…”

Section: Métodos De Tratamento De Efluentes Aquosos Coloridosunclassified

Adsorção de corantes aniônicos de solução aquosa em cinza leve de carvão e zeólita de cinza leve de carvão

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Decolorization and biodegradation of Indigo carmine by a textile soil isolate Paenibacillus larvae

Cited by 51 publications

References 17 publications

Biodecolorization and Bioremediation of Denim Industrial Wastewater by Adapted Bacterial Consortium Immobilized on Inert Polyurethane Foam (PUF) Matrix: A First Approach with Biobarrier Model

Biodecolorization and Bioremediation of Denim Industrial Wastewater by Adapted Bacterial Consortium Immobilized on Inert Polyurethane Foam (PUF) Matrix: A First Approach with Biobarrier Model

Decolorization of Direct Red 28 by mixed bacterial culture in an up-flow immobilized bioreactor

Adsorção de corantes aniônicos de solução aquosa em cinza leve de carvão e zeólita de cinza leve de carvão

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