Decolorization of textile azo dye and Congo red by an isolated strain of the dissimilatory manganese-reducing bacterium Shewanella xiamenensis BC01

Abstract

Shewanella xiamenensis BC01 (SXM) was isolated from sediment collected off Xiamen, China and was identified based on the phylogenetic tree of 16S rRNA sequences and the gyrB gene. This strain showed high activity in the decolorization of textile azo dyes, especially methyl orange, reactive red 198, and recalcitrant dye Congo red, decolorizing at rates of 96.2, 93.0, and 87.5%, respectively. SXM had the best performance for the specific decolorization rate (SDR) of azo dyes compared to Proteus hauseri ZMd44 and Aeromonas hydrophila NIU01 strains and had an SDR similar to Shewanella oneidensis MR-1 in Congo red decolorization. Luria-Bertani medium was the optimal culture medium for SXM, as it reached a density of 4.69 g-DCW L(-1) at 16 h. A mediator (manganese) significantly enhanced the biodegradation and flocculation of Congo red. Further analysis with UV-VIS, Fourier Transform Infrared spectroscopy, and Gas chromatography-mass spectrometry demonstrated that Congo red was cleaved at the azo bond, producing 4,4'-diamino-1,1'-biphenyl and 1,2'-diamino naphthalene 4-sulfonic acid. Finally, SEM results revealed that nanowires exist between the bacteria, indicating that SXM degradation of the azo dyes was coupled with electron transfer through the nanowires. The purpose of this work is to explore the utilization of a novel, dissimilatory manganese-reducing bacterium in the treatment of wastewater containing azo dyes.

Mobilization of Heavy Metals in Contaminated Soils induced by Bioaugmentation of Shewanella xiamenensis HM14

“…Phylogenetic tree (Fig. 1) shows the position of the isolated bacterium with respect to related species which is a facultative anaerobe with the ability to reduce cadmium as well as iron and manganese metabolically; that is, it can use iron and manganese as the terminal electron acceptor in the electron transport chain (Ng et al 2014). reaches to maximal at 60h after incubation.…”

Explored a cryptic plasmid pSXM33 from Shewanella xiamenensis BC01 and construction as the shuttle vector

“…The latest investigations on Shewanella have been focused on the metabolic pathways or application systems under different conditional stimuli [10,[14][15][16]. Studies on the molecular and genomic makeup of Shewanella are far behind those of common bacteria, mainly on account of the lack of effective vector systems and difficulties with DNA transformation.…”

“…In contrast, our previous study of a new strain, S. xiamenensis BC01 (SXM) showed good performance in the biodecolorization of azo dyes. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences revealed that the strain was most closely related to S. xiamenensis S4, S. oneidensis MR-1 and related species [10]. To clarify the connection between SXM and other strains, the draft genome of SXM was sequenced and characterized.…”

Adenosine monophosphate degradation and inosinic acid accumulation in the shrimp Penaeus japonicus

“…Exiguobacterium oxidotolerans has been isolated from a drain in a fish processing plant in Hokkaido (Yumoto et al 2004) and from sea mud (Chen et al 2010). Shewanella xiamenensis has been isolated from sea floor sediment (Ng et al 2014;Huang et al 2010). These three species of bacteria are present on the sea floor where shrimp live, so it was not surprising to encounter them in our samples.…”