Deconjugation of bile salts by Lactobacillus acidophilus isolates

Ahn, Young Tae; Kim, Geun Bae; Lim, Kwang‐Sei; baek, yong hyeon; Kim, Hyun Uk

doi:10.1016/s0958-6946(02)00174-7

Cited by 94 publications

(84 citation statements)

References 34 publications

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“…The effects of probiotic bacteria such as L. acidophilus on serum cholesterol levels have attracted much interest in recent years (Gilliland et al 1985;Buck & Gilliland 1994;Ahn et al 2003;Liong & Shah 2005;Park et al 2007;Mathara et al 2008). In the present study, we have shown that all the strains of the lactobacilli tested had the ability to remove cholesterol from laboratory media during growth.…”

Section: Discussionsupporting

confidence: 59%

Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China

Guo¹,

Liu²,

Gui-cheng³

2011

Czech J. Food Sci.

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A total of 38 strains were initially isolated from Jiaoke, a homemade traditional fermented cream, which was sourced from Inner Mongolia, China, and 6 strains were selected after screening, which was based on their cholesterol removal abilities. The Lactobacillus plantarum with the highest level of cholesterol removal from the media was identified using phenotypical characteristics and 16S rRNA sequences, and was named L. plantarum KLDS 1.0344. The mechanisms for cholesterol removal involved co-precipitation, assimilation, and degradation of cholesterol by L. plantarum KLDS 1.0344. At a level of 8.56 log CFU/ml, L. plantarum KLDS 1.0344 survived in pH 2.5 MRS broth for 2 h and exhibited excellent tolerance to 0.3% (w/v) bile. This strain has the most potential in applications as a dietary supplement for lowering human serum cholesterol. A series of tests on animal model and/or clinical tests will be conducted before it can be used in a pharmaceutical application.

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Section: Discussionsupporting

confidence: 59%

Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China

Guo¹,

Liu²,

Gui-cheng³

2011

Czech J. Food Sci.

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“…Con estos resultados se demuestra que las cepas de Lactobacillus en estudio pudieran también ser capaces de desconjugar las sales biliares. En estudios anteriores (Tanaka et al, 1999;Ahn et al, 2003;Liong y Shah, 2005) se ha comprobado que las bacterias ácido lácticas de los géneros Lactobacillus, Bifidobacterium, Lactococcus, Leuconostoc y Streptococcus son capaces de producir la enzima conocida como sal biliar hidrolasa (SBH), que cataliza la hidrólisis de las sales biliares conjugadas con glicina y taurina. Esta desconjugación pudiera ocurrir en la fase estacionaria del crecimiento bacteriano, ya que la actividad de la SBH se incrementa al disminuir el pH por producción de gran cantidad de ácidos orgánicos (Corzo y Gilliland, 1999).…”

Section: Discussionunclassified

AISLAMIENTO, IDENTIFICACIÓN Y CARACTERIZACIÓN PARCIAL DE LAS PROPIEDADES PROBIÓTICAS DE CEPAS DELactobacillussp. PROCEDENTES DEL TRACTO GASTROINTESTINAL DE POLLOS DE CEBA ISOLATION, IDENTIFICATION AND PARTIAL CHARACTERIZATION OF THE PROBIOTIC PROPERTIES OFLactobacillussp. STRAINS OBTAINED FROM THE GASTROINTESTINAL TRACT OF BROILERS

Rondón

Samaniego

Bocourt³

et al. 2008

Ciencia y Tecnologia Alimentaria

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“…However, current research data strongly suggest that microbial BSH function in the detoxification of bile salts increases the intestinal survival and persistence of producing strains, which in turn increases the overall beneficial effects associated with the strain [49,63]. Moreover, the bacterial genera that would most likely be used as probiotics (bifidobacteria and lactobacilli) are not capable of dehydroxylating de-conjugated bile salts [73][74][75], and thus the majority of the breakdown products of BSH activity by a probiotic strain may be precipitated and excreted in feces [76,77].…”

Section: Survival Under Conditions Simulating the Human Gi Tractmentioning

confidence: 98%

Characterization of Antibacterial Activity and Potential as Probiotic of Lactic Acid Bacteria Isolated from Goat’s Milk in Algeria

Benreguieg¹,

Dalache²,

Gacemi³

2013

JLS

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Lactic acid bacteria (LAB) were isolated from goat's milk in different north west region of Algeria. A total of 233 isolates were identified as LAB. However, only 11 strains showed excellent inhibition zones on agar when Salmonella typhimorium ATCC 13311 was used as an indicator for preliminary detection of antagonistic activity. After elimination of inhibition due to acid and H 2 O 2 , Staphylococcus aureus ATCC 25923 was used for secondary screening for antagonistic activity of these 11 strains. It was found that only 9 strains exhibited a good inhibition zones on agar, and all of them could inhibit E. coli ATCC 25921 and Pseudomonas aeruginosa ATCC 27853 as the third and the forth indicators respectively. Lactococcus lactis subsp. cremoris, Lactobacillus casei subsp. casei, Lactobacillus casei subsp. rhamnosus, Streptococcus salivarius subsp. thermophilus and Lactococcus lactis biovar.diacetylactis were the strains that have a good antibacterial activity, survive in the gastrointestinal conditions and were sensible to the majority of the antibiotics. All isolated strains tolerate pH = 2 and high bile salt concentration.

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Deconjugation of bile salts by Lactobacillus acidophilus isolates

Cited by 94 publications

References 34 publications

Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China

Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China

Characterization of Antibacterial Activity and Potential as Probiotic of Lactic Acid Bacteria Isolated from Goat’s Milk in Algeria

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