Decontamination of a BSL3 laboratory by hydrogen peroxide fumigation using three different surrogates for <i>Bacillus anthracis</i>
 spores

Kaspari, Oliver; Lemmer, Karin; Becker, S; Lochau, P.; Howaldt, Sabine; Nattermann, H.; Grunow, Roland

doi:10.1111/jam.12601

Cited by 17 publications

(24 citation statements)

References 30 publications

(84 reference statements)

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“…Survival of Geobacillus stearothermophilus spores was used to verify the disinfection efficiency of VHP in this study [11]. The effects of different injection rates and different injection times were investigated to determine optimal disinfection conditions.…”

Section: Determination Of Optimal Disinfection Conditions Using Vhpmentioning

confidence: 99%

“…VHP can automatically degrade into water and oxygen in nature, and therefore is friendly to the environment. Hydrogen peroxide decontamination systems are widely used to reduce the risk of infection in hospitals, biosafety laboratories, and so on [10,11]. Chou et al demonstrated that hydrogen peroxide had no effect on the mechanical properties of nylon, polyester, and Nomex [12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Disinfection efficiency of positive pressure respiratory protective hood using fumigation sterilization cabinet

Hao

Zhang

et al. 2019

Biosafety and Health

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Section: Determination Of Optimal Disinfection Conditions Using Vhpmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Disinfection efficiency of positive pressure respiratory protective hood using fumigation sterilization cabinet

Hao

Zhang

et al. 2019

Biosafety and Health

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“…Consequently, surrounding rooms have to be closed during the procedure. Furthermore, formamide forms toxic or irritant residues on surfaces, which subsequently need to be cleaned (Cheney and Collins, 1995;Krishnan et al, 2006;Gordon et al, 2012;Kaspari et al, 2014). Finally, since 2014, formamide is classified as a Class 1B carcinogen and Class 2 mutagen in Europe and has also been recognized as a human carcinogen in the United States (U.S Department of Health and Human Services, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, it has become more widely used than formamide for room-wide decontaminations in recent years. It has proven to be effective against a wide range of bacteria, spores, and viruses in various facility settings (Heckert et al, 1997;Krishnan et al, 2006;Pottage et al, 2010;Bentley et al, 2012;Goyal et al, 2014;Kaspari et al, 2014;Lemmen et al, 2015;Petit et al, 2017). Depending on the environmental conditions, H 2 O 2 can react as a weak acid, a potent oxidizing, or a reducing agent.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Hydrogen Peroxide Fumigation and Heat Treatment for Standard Emergency Arthropod Inactivation in BSL-3 Insectaries

Häcker

Koller²,

Eichner

et al. 2020

Front. Bioeng. Biotechnol.

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Climate change and global movements of people and goods have accelerated the spread of invasive species, including insects that vector infectious diseases, which threaten the health of more than half of the world’s population. Increasing research efforts to control these diseases include the study of vector – pathogen interactions, involving the handling of pathogen-infected vector insects under biosafety level (BSL) 2 and 3 conditions. Like microbiology BSL-3 laboratories, BSL-3 insectaries are usually subjected to fixed-term or emergency room decontamination using recognized methods such as hydrogen peroxide (H 2 O 2 ) or formaldehyde fumigation. While these procedures have been standardized and approved for the inactivation of diverse pathogens on surfaces, to date, there are no current standards for effective room-wide inactivation of insects in BSL-3 facilities in case of an emergency such as the accidental release of a large number of infected vectors. As H 2 O 2 is often used for standard room decontamination in BSL-3 facilities, we evaluated H 2 O 2 fumigation as a potential standard method for the safe, room-wide deactivation of insects in BSL-3 insectaries in comparison to heat treatment. To account for physiological diversity in vector insect species, six species from three different orders were tested. For the H 2 O 2 fumigation we observed a strong but also varying resilience across all species. Lethal exposure time for the tested dipterans ranged from nine to more than 24 h. Furthermore, the coleopteran, Tribolium castaneum , did not respond to continuous H 2 O 2 exposure for 48 h under standard room decontamination conditions. In contrast, temperatures of 50°C effectively killed all the tested species within 2 to 10 min. The response to lower temperatures (40–48°C) again showed a strong variation between species. In summary, results suggest that H 2 O 2 fumigation, especially in cases where a gas generator is part of the laboratory equipment, may be used for the inactivation of selected species but is not suitable as a general emergency insect inactivation method under normal room decontamination conditions. In contrast, heat treatment at 48 to 50°C has the potential to be developed as an approved standard procedure for the effective inactivation of insects in BSL-3 facilities.

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“…In addition, we describe a comprehensive, systematic method of facility-wide C. bovis eradication. All murine housing rooms; use, quarantine, storage, common procedural, surgical, imaging, genetic engineering, and pharmacy areas; and offices of the facility were decontaminated by using VHP (Bioquell, Horsham, PA) with room equipment left in place; this process was verified through the use of biologic and chemical indicators applied in healthcare, 1,11,15,16 biocontainment, 9 and animal facilities. 10,17 In addition, decontamination of surfaces, including benchtops, biosafety cabinets, animal transfer stations, gloved hands, imaging and anesthesia equipment, and the external surfaces of the occupied IVC before and after each use was accomplished by treatment with accelerated hydrogen peroxide (aHP) typically used in healthcare settings and validated as an effective, safe sanitizing agent (OxivirTb, Diversey, Charlotte, NC).…”

mentioning

confidence: 99%

Facility-wide Eradication of Corynebacterium bovis by using PCR-validated Vaporized Hydrogen Peroxide

Miedel

Ragland

Engelman

2018

j am assoc lab anim sci

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Facility-wide Corynebacterium bovis eradication was established using vaporized hydrogen peroxide (VHP) decontamination guided by C. bovis PCR surveillance. Prior attempts limited to culling PCR-positive mice and decontaminating affected rooms were ineffective in preventing recurrence. Because research aims often require trafficking to and use of procedural cores, a 12-mo facility-wide C. bovis PCR surveillance of 2064 specimens was performed and documented that, despite the presence of few clinically hyperkeratotic mice, 35% of the murine housing and use space was contaminated by C. bovis. The airways of IVC racks and air-handling units (AHU) provided a substantive niche for C. bovis survival, comparable to the primary enclosure, with 26% of murine and 22% of airway specimens PCR-positive for C. bovis. Equipment airway VHP sterilization in a 'flex room' required an 'active-closed' setting with the IVC rack connected to the AHU set to the VHP cycle, because 12% of specimens from 'static-open' VHP-exposed airways remained PCR-positive for C. bovis, whereas 0% of specimens from active-closed VHP exposures were positive. VHP decontamination of the 29,931-ft2 facility was completed in 2 mo. C. bovis PCR testing of IVC exhaust plenums for 200 d in previously C. bovis-affected rooms confirmed that none of the 259 specimens tested were PCR-positive for the organism. Monthly surveillance identified a single recurrence during June 2017 (month 9), ensuring rapid culling of C. bovis PCR-positive mice and acute VHP decontamination of equipment and rooms. Molecular persistence of C. bovis was resolved in procedural and personnel areas, and no murine or housing specimens tested C. bovis PCR-positive during study months 11 and 12. Furthermore, since the conclusion of the 12-mo study, none of the 452 additional murine, cell biologic, environmental, and monthly equipment surveillance specimens tested were C. bovis PCR-positive, documenting an 11-mo period of facility-wide C. bovis eradication to date. Study invalidation due to C. bovis can be avoided through PCR surveillance for the organism, immediate culling of PCR-positive mice, and acute VHP decontamination of affected areas.

show abstract

Decontamination of a BSL3 laboratory by hydrogen peroxide fumigation using three different surrogates for Bacillus anthracis spores

Abstract: The results of this study provide important results in the field of hydrogen peroxide decontamination when analysing the effect on spores other than those of G. stearothermophilus.

Cited by 17 publications

References 30 publications

Disinfection efficiency of positive pressure respiratory protective hood using fumigation sterilization cabinet

Disinfection efficiency of positive pressure respiratory protective hood using fumigation sterilization cabinet

Evaluation of Hydrogen Peroxide Fumigation and Heat Treatment for Standard Emergency Arthropod Inactivation in BSL-3 Insectaries

Facility-wide Eradication of Corynebacterium bovis by using PCR-validated Vaporized Hydrogen Peroxide

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