Decorating graphene oxide/nanogold with dextran-based polymer brushes for the construction of ultrasensitive electrochemical enzyme biosensors

Boujakhrout, Abderrahmane; Sánchez, Alfredo; Díez, Paula; Jiménez-Falcao, Sandra; Martı́nez-Ruiz, Paloma; Peña-Álvarez, Miriam; Pingarrón, José M.; Villalonga, Reynaldo

doi:10.1039/c5tb00451a

Cited by 37 publications

(11 citation statements)

References 49 publications

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“…Detection methods for biosensors include, micro-ring resonators [176], voltammetry [177][178][179], electrochemical detection [89,92,180], interferometric detection of scattering (iSCAT) [181], as well as secondary label methods such as ELISA [182] and fluorescence [174]. Methods such as fluorescence and ELISA can have a high degree of specificity and are amenable to microarray applications allowing detection of multiple components at once (Figure 14) [183,184].…”

Section: Modification and Detection Of Biological Componentsmentioning

confidence: 99%

From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes

et al. 2015

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Abstract:In this review, we describe the latest advances in synthesis, characterization, and applications of polymer brushes. Synthetic advances towards well-defined polymer brushes, which meet criteria such as: (i) Efficient and fast grafting, (ii) Applicability on a wide range of substrates; and (iii) Precise control of surface initiator concentration and hence, chain density are discussed. On the characterization end advances in methods for the determination of relevant physical parameters such as surface initiator concentration and grafting density are discussed. The impact of these advances specifically in emerging fields of nano-and bio-technology where interfacial properties such as surface energies are controlled to create nanopatterned polymer brushes and their implications in mediating with biological systems is discussed.

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Section: Modification and Detection Of Biological Componentsmentioning

confidence: 99%

From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes

et al. 2015

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“…These carbon nanomaterials showing a planar two-dimensional morphology can be prepared at relative low cost and easily functionalized with a variety of chemical groups. Graphene and its oxidized derivative can be also combined with other materials and compounds, such as polymers, ceramics, metal and metal oxides nanostructures, etc., to yield novel composite or hybrid adducts with improved or new chemical and physical properties [11][12][13]. In this regard, the synergistic effect between graphene and metal oxide nanostructures in hybrid nanomaterials have opened new possibilities to design electrochemical biosensors with desired properties and behaviors [14].…”

Section: Introductionmentioning

confidence: 99%

Reduced graphene oxide-Sb2O5 hybrid nanomaterial for the design of a laccase-based amperometric biosensor for estriol

Cincotto

Canevari

Machado

et al. 2015

Electrochimica Acta

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Reduced graphene oxide-Sb2O5 hybrid nanomaterial for the design of a laccase-based amperometric biosensor for estriol, Electrochimica Acta http://dx. AbstractA novel reduced graphene oxide/Sb2O5 hybrid nanomaterial was prepared by a one-pot reaction process. The nanomaterial was characterized by different spectroscopic, microscopic and electrochemical techniques, demonstrating that the graphene sheets were doped with a Sb2O5 thin film. A glassy carbon electrode coated with the hybrid material was further employed as support for the covalent immobilization of laccase to develop an electrochemical biosensor for estriol. The enzyme biosensor showed high sensitivity (275 mA/M) and fast analytical response (4 s) for the hormone, with a limit of detection of 11 nM in the range of 25 nM to 1.03 µM. The biosensor showed high selectivity for the analytical detection of estriol.

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“…The copper enzyme tyrosinase converts a broad spectrum of phenolic compounds using the ambient oxygen as the electron acceptor . Among them PAR is converted in two consecutive one‐electron oxidation steps into N‐acetyl‐p‐benzoquinone (4‐AOBQ) .…”

Section: Resultsmentioning

confidence: 99%

MIP‐esterase/Tyrosinase Combinations for Paracetamol and Phenacetin

Yarman

Scheller

2016

Electroanalysis

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A new electrochemical MIP sensor for the most frequently used drug paracetamol (PAR) was prepared by electropolymerization of mixtures containing the template molecule and the functional monomers o‐phenylenediamine, resorcinol and aniline. The imprinting factor of 12 reflects the effective target binding to the MIP as compared with the non‐imprinted electropolymer. Combination of the MIP with a nonspecific esterase allows the measurement of phenacetin – another analgesic drug. In the second approach the PAR containing sample solution was pretreated with tyrosinase in order to prevent electrochemical interferences by ascorbic acid and uric acid. Interference‐free indication at a very low electrode potential without fouling of the electrode surface was achieved with the o‐phenylenediamine: resorcinol‐based MIP.

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Decorating graphene oxide/nanogold with dextran-based polymer brushes for the construction of ultrasensitive electrochemical enzyme biosensors

Abstract: A novel strategy to prepare a water-soluble graphene derivative by attaching dextran polymer brushes.

Cited by 37 publications

References 49 publications

From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes

From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes

Reduced graphene oxide-Sb2O5 hybrid nanomaterial for the design of a laccase-based amperometric biosensor for estriol

MIP‐esterase/Tyrosinase Combinations for Paracetamol and Phenacetin

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