Decoupling analysis of the production mechanism of aqueous reactive species induced by a helium plasma jet

Chen, Zeyu; Liu, Dingxin; Xu, Han; Xia, Wenjie; Liu, Zhijie; Xu, Dehui; Rong, Mingzhe; Kong, Michael G.

doi:10.1088/1361-6595/ab006b

Cited by 40 publications

(29 citation statements)

References 53 publications

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“…The schematic diagram of the experimental setup is shown in Figure 13. The validation of this experimental setup was proved in [31]. Cell C, which did not have a copper grid and wire, was studied as the control group.…”

Section: Resultsmentioning

confidence: 99%

Aqueous Gold Nanoparticles Generated by AC and Pulse-Power-Driven Plasma Jet

Xie

Wu³

et al. 2019

Nanomaterials

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In this study, we developed a simple-to-use approach based on an atmospheric pressure plasma jet to synthesize aqueous Au nanoparticles (AuNP). Special attention was paid to the different reaction dynamics and AuNP properties under AC and pulse-power-driven plasma jets (A-Jet and P-Jet, respectively). The morphology of the AuNP, optical emissions, and chemical reactions were analyzed. Further, a copper mesh was placed above the reaction cell to evaluate the role of electrons and neutral species reduction. A visible color change was observed after the A-Jet treatment for 30 s, while it took 3 min for the P-Jet. The A-Jet treatment presented a much higher AuNP growth rate and a smaller AuNP diameter compared with the P-Jet treatment. Further analysis revealed an increase in chemical concentrations (Cl− and H2O2) and liquid conductivity after plasma treatment, with a higher increased amplitude for the A-Jet case. Moreover, the electrons alone had little effect on AuNP generation, while neutral species showed a clear Au+ reduction effect, and a unique coupling effect between both reactions was observed. The different reaction dynamics between the A-Jet and P-Jet were attributed to their different local heating effects and different discharge power during the reaction.

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Section: Resultsmentioning

confidence: 99%

Aqueous Gold Nanoparticles Generated by AC and Pulse-Power-Driven Plasma Jet

Xie

Wu³

et al. 2019

Nanomaterials

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“…CAP can be generated without requiring a vacuum environment [ 6 ], and the apparent temperature of CAP is close to room temperature, making it possible to directly treat the cells or tissues without causing thermal damage [ 7 , 8 , 9 ]. CAP can be used to generate reactive oxygen species (ROS) and reactive nitrogen species (RNS), including H 2 O 2 , NO 2 − , NO 3 − , O 3 , ONOO − , • OH, and O 2 •− [ 10 , 11 , 12 , 13 , 14 ], which play important roles in biomedical applications [ 15 , 16 ], such as cancer therapy [ 17 , 18 , 19 ], hemostasis [ 20 ], wound healing [ 21 , 22 , 23 ], and skin disease [ 24 ]. CAP is highly efficient for inactivation of different kinds of bacteria such as Gram-positive, Gram-negative bacteria and fungus [ 25 , 26 ], as well as the drug-resistant microorganisms and bacterial biofilms [ 27 , 28 ], which is benefit for the bacterial infected wound.…”

Section: Introductionmentioning

confidence: 99%

Effects of Plasma-Activated Water on Skin Wound Healing in Mice

Wang

et al. 2020

Microorganisms

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Cold atmospheric plasma (CAP) has been widely used in biomedicine during the last two decades. While direct plasma treatment has been reported to promote wound healing, its application can be uneven and inconvenient. In this study, we first activated water with a portable dielectric barrier discharge plasma device and evaluated the inactivation effect of plasma-activated water (PAW) on several kinds of bacteria that commonly infect wounds. The results show that PAW can effectively inactivate these bacteria. Then, we activated tap water and examined the efficacy of PAW on wound healing in a mouse model of full-thickness skin wounds. We found that wound healing in mice treated with PAW was significantly faster compared with the control group. Histological analysis of the skin tissue of mice wounds showed a significant reduction in the number of inflammatory cells in the PAW treatment group. To identify the possible mechanism by which PAW promotes wound healing, we analyzed changes in the profiles of wound bacteria after PAW treatment. The results show that PAW can significantly reduce the abundance of wound bacteria in the treatment group. The results of biochemical blood tests and histological analysis of major internal organs in the mice show that PAW had no obvious side effects. Taken together, these results indicate that PAW may be a new and effective method for promoting wound healing without side effects.

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“…It has been extensively proven that there are types of reactive species (such as energy ions, excited molecules, free radicals, electric fields, and UV radiation) in the gas phase of CAP [ 59 ]. When CAP irradiates cells cultured in vitro or living tissues, the gas-phase reactive species can be trapped by the medium or blood surrounding the cells, where they then initiate a series of reactions to generate a large number of liquid-phase ROS and RNS, such as ozone (O 3 ), hydrogen peroxide (H 2 O 2 ), hydroxide radicals (OH•), superoxide anion radicals (O 2 − ), nitrite ions (NO 2 − ), nitrate ions (NO 3 − ), peroxynitrite (ONOOH/ONOO − ), and singlet oxygen (O 2 ( 1 Δg)) [ 49 , 60 , 61 , 62 ]. These CAP-generated exogenous ROS and RNS can cause the accumulation of intracellular ROS levels, and eventually trigger programmed cell death.…”

Section: Discussionmentioning

confidence: 99%

The Antitumor Effects of Plasma-Activated Saline on Muscle-Invasive Bladder Cancer Cells In Vitro and In Vivo Demonstrate Its Feasibility as a Potential Therapeutic Approach

Zhang

Guo

et al. 2021

Cancers

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Muscle-invasive bladder cancer (MIBC) is a fast-growing and aggressive malignant tumor in urinary system. Since chemotherapy and immunotherapy are only useable with a few MIBC patients, the clinical treatment of MIBC still faces challenges. Here, we examined the feasibility of plasma-activated saline (PAS) as a fledgling therapeutic strategy for MIBC treatment. Our data showed that plasma irradiation could generate a variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in saline. In vivo tests revealed that pericarcinomatous tissue injection with PAS was effective at preventing subcutaneous bladder tumor growth, with no side effects to the visceral organs after long-term administration, as well as having no obvious influence on the various biochemistry indices of the blood in mice. The in vitro studies indicated that adding 30% PAS in cell culture media causes oxidative damage to the bladder transitional cells T24 and J82 through enhancing the intracellular ROS level, and eventually induces cancer cells’ apoptosis by activating the ROS-mediated Fas/CD95 pathway. Therefore, for an intracavity tumor, these initial observations suggest that the soaking of the tumor tissue with PAS by intravesical perfusion may be a novel treatment option for bladder cancer.

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Decoupling analysis of the production mechanism of aqueous reactive species induced by a helium plasma jet

Cited by 40 publications

References 53 publications

Aqueous Gold Nanoparticles Generated by AC and Pulse-Power-Driven Plasma Jet

Aqueous Gold Nanoparticles Generated by AC and Pulse-Power-Driven Plasma Jet

Effects of Plasma-Activated Water on Skin Wound Healing in Mice

The Antitumor Effects of Plasma-Activated Saline on Muscle-Invasive Bladder Cancer Cells In Vitro and In Vivo Demonstrate Its Feasibility as a Potential Therapeutic Approach

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