Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: Comparison between two extenders

Stradaioli, G.; Noro, Tadataka; Sylla, Lakamy; Monaci, Maurizio

doi:10.1016/j.theriogenology.2007.01.009

Cited by 116 publications

(95 citation statements)

References 31 publications

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“…Our results showed that better results of the cold shock test were achieved using the AndroMed® and Bioxcell®. This is in accordance with Jannet et al (2005) or Stradaioli et al (2007) Key: BULL = eff ect of each bull; DIL = eff ect of each diluter; LDLC = eff ect of diff erent concentration of LDL in tested sample; L0 = percentage rate of live sperm at the beginning of the test; L2 = percentage rate of live sperm a er 2 hours of the test duration; L0 -L2 = diff erence between percentage rate of live sperm at time 0 and 2 hours of the test. Diff erent superscript letters mean a signifi cant diff erence within a column -a, b = P < 0.05; A, B, C = P < 0.01…”

Section: Resultssupporting

confidence: 94%

Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Beran¹,

Šimoník²,

Stádník³

et al. 2013

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The objectives of this study were to determine and evaluate the eff ect of bull, diluter and addition of LDL in diff erent concentration on the percentage rate of spermatozoa survival a er cold shock. In total, four bulls were collected during a period of eight weeks. A total of 8 samples of fresh semen with required quality were processed. Three extenders were used for dilution of each sample; AndroMed®, Bioxcell® and Triladyl®, each in standard and LDL enriched variants. In the case of AndroMed® and Bioxcell®, 4, 6 and 8% of LDL were simply added. In Triladyl®, 6, 8 and 10% of LDL replaced the standard egg yolk component. Resistance of spermatozoa against cold shock (0 °C, 10 minutes) was evaluated by the percentage rate of live sperm using Eosin-Nigrosine staining immediately and 2 hours a er heat incubation (37 °C). The results showed the infl uence of bull individuality as an important factor. Among diluters used it is possible to recommend AndroMed® and Bioxcell® due to signifi cantly (P < 0.01) lower decline of live sperm proportion during the cold shock test than Triladyl® (−9.19, respectively −4.95%). The optimal LDL concentration increasing resistance of spermatozoa against cold shock was not determined, therefore subsequent research is necessary. bull semen, sperm survival, cold shock, extender, LDL cholesterol

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Section: Resultssupporting

confidence: 94%

Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Beran¹,

Šimoník²,

Stádník³

et al. 2013

Acta Univ. Agric. Silvic. Mendelianae Brun.

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“…Bioxcell was also used in the study of Celeghini et al (2008), who reported the same conclusions -that the other extenders which they evaluated were significantly better than Bioxcell. A contrary finding by Stradaioli et al (2007) that Bioxcell achieved significantly (P<0.05) better results was not confirmed in our observation. At the same time, we cannot confirm the conclusions of Herold et al (2000) and Janett et al (2005), who reported AndroMed to be the most suitable extender for cryopreservation of bull semen.…”

Section: Extender Effect On Traits Of Sperm Activity After Thawingcontrasting

confidence: 99%

Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

et al. 2012

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The objectives were to evaluate the effects of sire and content of fresh or ionised egg yolk in extenders on sperm motility and share of live and dead sperm in collected ejaculate, in thawed artificial insemination (AI) doses, and during thermodynamic testing compared to extenders not containing egg yolk. Ejaculates were collected once a week from 4 Holstein bulls. Each of the 20 ejaculate samples from each bull was diluted with 4 different extenders. AndroMed and Bioxcell (no egg yolk) and Triladyl and Optidyl (fresh, ionised egg yolk) were used. A total of 640 AI doses were analysed. The volume of samples, sperm concentration, and percentage of motile spermatozoa were evaluated after collection, as was sperm motility after thawing of AI doses and during thermodynamic testing. Percentages of live and dead sperm were also evaluated. The data set was analysed using SAS/STAT 9.1 (SAS Institute Inc., Cary, NC, USA). The results confirmed significant (P<0.05-0.01) between-sire differences in the volume, density, and activity of sperm as well as in share of live and dead sperm after collection; in decline of sperm motility in the fresh ejaculate, after thawing, and during the entire thermodynamic test, as well as in the share of live and dead sperm after thawing. The extenders ranked by sperm motility are: Optidyl, Triladyl, AndroMed, and Bioxcell, demonstrating the higher quality of AI doses produced using egg yolk extenders. Differences in sperm motility were significant (P<0.05-0.01) during the entirety of thermodynamic testing. Egg yolk extenders had a significantly (P<0.05-0.01) higher percentages of live sperm after thawing.

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“…This is in accordance with Stradaioli et al (2007) who state AndroMed ® and Bioxcell ® as the most suitable extenders for cryopreservation of bull semen. However, the general course of reproduction results in cows points out the necessity of continuous improving of extenders.…”

supporting

confidence: 88%

Influence of selected factors on bovine spermatozoa cold shock resistance

et al. 2015

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The objectives of this study were to determine the effects of sire, extender, and addition of Low Density Lipoprotein (LDL) to extenders used on the percentage rate of spermatozoa survival after cold shock. Two groups of extenders were compared: without LDL addition (control variants) and LDL enriched (experimental variants). Three extenders were used: AndroMed ® extenders, and 6-10% LDL addition into the Triladyl ® extender. In total, 12 samples of fresh semen were collected from 4 bulls during a period of 8 weeks. Bovine spermatozoa cold shock resistance (1 ± 1 °C, 10 min) was evaluated by the percentage rate of live sperm using eosin-nigrosine staining immediately and after heat incubation (37 ± 1 °C, 120 min). The results showed the effect of sire as important and individual differences between selected sires in their sperm resistance against cold shock were confirmed. AndroMed ® and Bioxcell ® were found to be providing better protection of bull semen to cold shock compared to Triladyl ® due to lower decline of live sperm proportion. Our results detected a positive effect of LDL addition on sperm resistance against cold shock, especially on lower decrease of live sperm percentage rate after 120 min of the heat test (P < 0.05). Further studies are needed to assess the optimal concentration of LDL in various kinds of extenders as well to state ideal time and temperature conditions for ensuring LDL reaction with sperm. , bull sperm, extender, LDL cholesterol, sperm survival, cold shock, eosin-nigrosine staining Reproduction

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Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: Comparison between two extenders

Cited by 116 publications

References 31 publications

Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

Influence of selected factors on bovine spermatozoa cold shock resistance

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