Decrease in Ketonemia Due to Infusion of Lipids in Pancreatectomized Dogs

Abstract

Pancreatectomized diabetic dogs, deprived of insulin treatment and anesthetized, were infused intravenously for one hour with a fatty emulsion (Lipomul) and kept under observation for six hours. During this period there was a significant decrease in ketonemia and prolonged persistence of hyperlipemia when compared with normal dogs similarly infused. DIABETES 14:33-35, January 1965.In a previous paper 1 the influence of hormonal factors on diabetic ketosis of pancreatectomized dogs was studied. In most of the cases hyperlipemia appeared before ketonemia. There were, however, certain exceptions; the most remarkable one was seen in pancreatectomized-hypophysectomized dogs, in which the injection of cortisol provoked marked hyperlipemia, but was not followed by an increase in ketonemia. This fact led us to study the effects of hormones on pancreatectomized dogs in which lipids were injected intravenously. MATERIAL AND METHODSTwenty-five dogs were used; fourteen were subjected to total pancreatectomy and eleven served as controls. The experiments were performed five to seven days after the operation and approximately sixteen hours after the last meal. The dogs weighed 4 to 18 kg., and were anesthetized with sodium pentobarbital (33 mg. per kg. of body weight).The pancreatectomized dogs were fed with 40 gm. per kg. of raw heart beef and 70 gm. of raw pancreas daily.The animals were divided into four groups as follows: (I) normal dogs; (II) normal dogs infused with Lipomul* for one hour; (III) totally pancreatecto-

Contractile activity and prostacyclin generation in isolated coronary arteries from diabetic dogs

“…Three different groups of animals were used: a) normal (n = 6); b) totally pancreatectomized (n = 6) and c) totally pancreatectomized injected with insulin (n = 3). Experiments were performed 5-7 days after the operation and approximately 16 h following the last meal [13]. The pancreatectomized dogs were fed with raw beef heart ad libitum, and 70 g of raw pancreas daily [13].…”

“…Experiments were performed 5-7 days after the operation and approximately 16 h following the last meal [13]. The pancreatectomized dogs were fed with raw beef heart ad libitum, and 70 g of raw pancreas daily [13]. Normal and insulin-treated dogs were fed with the same food.…”

Ver�nderungen des Gehaltes der freien Fetts�uren und der Ketonk�rper im Blut alloxandiabetischer Ratten nach i.v. Fettbelastung und Heparinapplikation

“…Verschiedene Angaben aus der Literatur bestgtigen, dag nach langdauernder Fettfiitterung bei chronisch Mloxandiabetischen Ratten [2,11] sowie nach einer i. v. Fettbelastung bei pancreatectomierten [22,3] oder Mloxandiabetischen Hunden [6] exogenes Fett langsamer aus dem Blur verschwindet. Dieses Phgnomen wird h/tufig darauf zur~ickgefiihrt, dab beim Alloxanoder Pancreasdiabetes sowohl die Lipoproteinlipase (LPL)-Aktivit/tt des Fettgewebes [15,20,7], als auch die Postheparin-LPL-Aktivitgt des Plasmas [12] verminder~ ist.…”

“…In der uns zug/~ngliehen Literatur wurden /ihnliche Versuehe nur bei Hunden im akuten oder subakuten Stadium des Diabetes durchgefiihrt [3,6]. In diesen Stadien ist, wie aus den Angaben der Autoren hervorgeh~, der FFA-GehMt des Blutes schon vor der Fettinfusion erh6ht.…”

Das Verhalten von Serumlipiden alloxandiabetischer und normaler Ratten nach verschiedenartiger Applikation einer Fettemulsion

“…Diese Ergebnisse könnten darauf hinweisen, daß bei diabetischer .Stoffwechsellage nur nach peroraler Zufuhr von Fett eine besondere Verwertungsstörung auftritt. Zahlreiche Literat urangaben weisen jedoch auf ein verlangsamtes Ver schwinden infundierter Fettemulsionen und Chylomikronen aus dem Blut dia betischer Patienten und von Tieren mit experimentellem Diabetes hin (10,14,11,5,8,4).…”