Decrease of carbohydrate in membrane glycoproteins during human erythrocyte ageing in vivo

Gattegno, Liliane; Perret, G; Fabia, F; Cornillot, P

doi:10.1016/0047-6374(81)90097-x

Cited by 18 publications

(9 citation statements)

References 22 publications

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“…The amount of sialic acid released enzymatically is correlated with the total sialic acid residues on the erythrocyte surface (71). The erythrocyte surface sialic acid has been shown to determine the life span of erythrocytes (72)(73)(74)(75). It has been shown that desialylated erythrocytes are rapidly cleared from blood stream in rabbits (76) and man (77,78).…”

Section: Erythrocyte Sialic Acidmentioning

confidence: 99%

Sialic acid in cardiovascular diseases

Nigam

Narain

Kumar

2006

Indian J Clin Biochem

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Sialic acid, the acylated derivatives of 9-carbon sugar neuraminic acid, present as terminal component of oligosaccharide chains of many glycoproteins and glycolipids, has been recognized to be involved in the regulation of a great variety of biological phenomena. Studies have shown that serum sialic acid predicts both coronary heart disease and stroke mortality and reflects the existence or activity of an atherosclerotic process. Most of the studies have shown an elevation in serum sialic acid concentration in coronary heart disease and a positive correlation between the raised serum sialic acid and the severity of the coronary lesions is observed. However, a few contradictory reports are also available. Racial differences in serum sialic acid have also been reported and correlated with international differences in the prevalence of atherosclerosis. Reduced sialic acid content of platelets, erythrocytes and lipoproteins may play important role in the pathogenesis of atherosclerosis. Elucidation of the mechanism of alternation in sialic acid concentration may throw more light on its potential clinical utility. Hence more studies are needed to designate sialic acid as a cardiovascular risk factor / marker.

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Section: Erythrocyte Sialic Acidmentioning

confidence: 99%

Sialic acid in cardiovascular diseases

Nigam

Narain

Kumar

2006

Indian J Clin Biochem

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“…Decreased content of neutral hexoses, fucose. mannose, galactose, galactosamine, glucose, glucos amine and 2-acetamido-2-deoxyglucose resi dues (per cell) were observed [90,[95][96][97][98], No significant differences were noted when the results were expressed as the numbers of res idues per 100 carbohydrate residues, sug gesting a homogeneous decrease of various carbohydrate residues during erythrocyte aging [95]. These results could be accounted for by microvesiculation although a recent lectin binding study showed a decrease in the number of receptor sites for a variety of lec tins (per unit surface area of red blood cells) without any significant change in the density of receptors for Trilicum vulgaris lectin [99], Similarly, immunological studies point to small decreases in the amounts of B and H but not A, M and N antigens during in vivo aging of the human erythrocyte [100].…”

Section: Carbohydrate Compositionmentioning

confidence: 99%

Erythrocyte Aging: Physical and Chemical Membrane Changes

Bartosz¹

1991

Gerontology

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The mammalian erythrocyte is an interesting model for studies of membrane aging. Experimental approaches to this problem involve, first of all, comparison of properties of erythrocytes separated by density because red blood cell age correlates with density in principle. Other approaches to study red cell membrane aging, such as hypertransfusion, are also discussed. A number of physical and chemical changes occur in erythrocytes with aging. Crucial to the elucidation of aging mechanisms is to determine which are primary and which are secondary. Immunoglobulin G binding triggers cellular removal and seems necessary for the recognition of senescent erythrocytes. Cellular deformability decreases while fragility to hemolytic factors generally increases and surface charge density does not alter. Perhaps more important are reactions of reactive oxygen species with membrane constituents and subsequent proteolysis. These are implicated as causative factors in red cell aging, immunoglobulin binding and recognition of senescent erythrocytes.

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“…While aging, erythrocytes lose sialic acids [25][26][27][28] among other surface carbohydrates. As a reduction of sialic acids on the erythrocyte's surface is known to be associated with elevated erythro-phagocytosis, 16 we quantified sialic acid on wt and tg6 erythrocytes.…”

Section: Assessment Of Properties Typical For Senescent Erythrocytesmentioning

confidence: 99%

Enhanced erythro-phagocytosis in polycythemic mice overexpressing erythropoietin

Bogdanova

Mihov

Lutz

et al. 2007

Blood

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Adaptive mechanisms to hematocrit levels of 0.9 in our erythropoietin-overexpressing mice (tg6) include increased plasma nitric oxide levels and erythrocyte flexibility. Doubled reticulocyte counts in tg6 suggest an increased erythrocyte turnover. Here we show that compared with wild-type (wt) animals, erythrocyte lifespan in tg6 is 70% lower in tg6 mice. Transgenic mice have a younger erythrocyte population as indicated by higher intercellular water and potassium content, higher flexibility, decreased density, increased surface to volume ratio, and decreased osmotic fragility. Interestingly, despite being younger, the tg6 erythrocyte population also harbors characteristics of accelerated aging such as an increased band 4.1a to 4.1b ratio, signs of oxidative stress, or decreased surface CD47 and sialic acids. In tg6, in vivo tracking of PKH26-labeled erythrocytes revealed dramatically increased erythrocyte incorporation by their liver macrophages. In vitro experiments showed that tg6 macrophages are more active than wt macrophages and that tg6 erythrocytes are more attractive for macrophages than wt ones. In conclusion, in tg6 mice erythrocyte aging is accelerated, which results, together with an increased number and activity of their macrophages, in enhanced erythrocyte clearance. Our data points toward a new mechanism downregulating red cell mass in excessive erythrocytosis in mice. IntroductionDuring aging, the erythrocytes considerably change the internal ion and protein composition, and the biomechanical and biochemical properties of their cell membrane, as well as size, shape, and surface to volume ratio. In humans, these changes finally result in selective elimination of senescent erythrocytes from the circulation. 1 The clearance of erythrocytes in mice and rabbits is in part age dependent and in part random. 2 Nevertheless, the agedependent erythrocyte clearance in these mammals is well controlled, involving oxidative damage, phosphatidylserine exposure, 3,4 desialylation, 5 and immunoglobulins. 6 The involvement of immunoglobulins in clearance of senescent erythrocytes in mice must, however, differ from that in humans, because naturally occurring antibodies in mice are exclusively of the IgM class. Under pathological conditions such as sickle cell anemia the phosphatidylserine exposure to the outer leaflet of the red cell membrane presumably plays a predominant role since in this disease the percentage of phosphatidylserine-expressing red cells is increased 2-to 10-fold. 4 Our transgenic (tg6) mice that constitutively overexpress human Epo in an oxygen-independent manner have a 12-fold elevated Epo plasma level 7 leading to hematocrit values of up to 0.9. As to the question how tg6 mice cope with excessive erythrocytosis, we reported earlier that they show chronic vasodilatation due to excessive NO production 7 and regulate blood viscosity by elevating erythrocyte flexibility. 8 Here we were especially interested to define the process of erythrocyte turnover in tg6 animals by focusing on mechanisms of...

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Decrease of carbohydrate in membrane glycoproteins during human erythrocyte ageing in vivo

Cited by 18 publications

References 22 publications

Sialic acid in cardiovascular diseases

Sialic acid in cardiovascular diseases

Erythrocyte Aging: Physical and Chemical Membrane Changes

Enhanced erythro-phagocytosis in polycythemic mice overexpressing erythropoietin

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