Decrease of contractile properties and transversal stiffness of single fibers in human soleus after 7-day “dry” immersion

Пономарева, Е. В.; Kartashkina, N. L.; Altaeva, E. G.; Fokina, N M; Kurushin, V. A.; Kozlovskaya, I. B.; Шенкман, Борис

doi:10.1016/j.actaastro.2010.01.012

Cited by 17 publications

(23 citation statements)

References 27 publications

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“…In 2002 and 2009, we studied the effect of 7-day “dry” immersion on the contractile properties of human soleus muscle fibers. In the first series of experiments, maximal tension of soleus muscle fibers decreased by 32% (Shenkman et al., 2004), and in the second series of experiments, it decreased by 26% (Ogneva et al., 2011a,b). No significant alterations in specific tension were noted.…”

Section: Contractile Properties Of Single Permeabilized Fibersmentioning

confidence: 99%

“…We used atomic force microscopy for the first study of the transversal stiffness of the various compartments of human soleus muscle fibers after 7 days of “dry” immersion (Ogneva et al., 2011a,b). The measurements were performed according to the original protocol (Ogneva et al., 2010) on single permeabilized fibers in the relaxed state and at the peak of isometric activity with pCa = 4.2.…”

Section: Transversal Stiffness Of Myofibrillar Apparatusmentioning

confidence: 99%

“…After 7-day “dry” immersion soleus muscle samples exhibited a 20% decrease in the content of desmin (Ogneva et al., 2011a,b), a protein interlinking myofibrils in the Z-disc region and other cellular compartments (mitochondria, myonuclei, etc.) (see review by Capetanaki et al., 2007).…”

Section: Myofibrillar Cytoskeletal Proteinsmentioning

confidence: 99%

“…Thus, atrophy development was prevented without usage of intense running or resistance exercise. Plantar stimulation allowed to prevent decrease in maximal isometric tension and calcium sensitivity in permeabilized muscle fibers (Figure 1; Litvinova et al., 2004; Shenkman et al., 2004; Ogneva et al., 2011a,b). These findings give evidence that muscle activity induced by support afferent stimulation could prevent malformation of cross-bridges.…”

Section: The Role Of Support Afferentation In the Development Of Hypomentioning

confidence: 99%

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Cellular Responses of Human Postural Muscle to Dry Immersion

Шенкман

Kozlovskaya

2019

Front. Physiol.

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Support withdrawal has been currently considered as one of the main factors involved in regulation of the human locomotor system. For last decades, several authors, including the authors of the present paper, have revealed afferent mechanisms of support perception and introduced the concept of the support afferentation system. The so-called “dry immersion” model which was developed in Russia allows for suspension of subjects in water providing the simulation of the mechanical support withdrawal. The present review is a summary of data allowing to appreciate the value of the “dry” immersion model for the purposes of studying cellular responses of human postural muscle to gravitational unloading. These studies corroborated our hypothesis that the removal of support afferentation inactivates the slow motor unit pool which leads to selective inactivation, and subsequent atony and atrophy, of muscle fibers expressing the slow isoform of myosin heavy chain (which constitutes the majority of soleus muscle fibers). Fibers that have lost a significant part of cytoskeletal molecules are incapable of effective actomyosin motor mobilization which leads to lower calcium sensitivity and lower range of maximal tension in permeabilized fibers. Support withdrawal also leads to lower efficiency of protective mechanisms (nitric oxide synthase) and decreased activity of AMP-activated protein kinase. Thus, “dry” immersion studies have already contributed considerably to the gravitational physiology of skeletal muscle.

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Section: Contractile Properties Of Single Permeabilized Fibersmentioning

confidence: 99%

Section: Transversal Stiffness Of Myofibrillar Apparatusmentioning

confidence: 99%

Section: Myofibrillar Cytoskeletal Proteinsmentioning

confidence: 99%

Section: The Role Of Support Afferentation In the Development Of Hypomentioning

confidence: 99%

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Cellular Responses of Human Postural Muscle to Dry Immersion

Шенкман

Kozlovskaya

2019

Front. Physiol.

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“…Short and long-term exposures to microgravity analogs are known to alter skeletal neuromuscular performance in weight and non-weight bearing muscle groups, also known as load- and non-load bearing muscles. The longer the exposure to the WI unloading environment, the greater the alteration to performance, neuromuscular signaling, and muscle microanatomy ( Desaphy et al, 2005 ; Ogneva et al, 2011 ; Cotter et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of Resting, Consecutive, Long-Duration Water Immersions on Neuromuscular Endurance in Well-Trained Males

et al. 2018

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Purpose: This study examined the effects of repeated long-duration water immersions (WI)s at 1.35 atmospheres absolute (ATA) on neuromuscular endurance performance. We hypothesized that, following 5 days of consecutive, resting, long-duration WIs, neuromuscular endurance performance would decrease.Methods: Fifteen well-trained, male subjects completed five consecutive 6-h resting WIs with 18-h surface intervals during the dive week while breathing compressed air at 1.35 ATA. Skeletal muscle endurance performance was assessed before and after each WI, and 24 and 72 h after the final WI. Muscular endurance assessments included 40% maximum handgrip endurance (MHE) and 50-repetition maximal isokinetic knee extensions. Near infrared spectroscopy was used to measure muscle oxidative capacity of the vastus lateralis and localized muscle tissue oxygenation of the vastus lateralis and flexor carpi radialis. Simultaneously, brachioradialis neuromuscular activation was measured by surface electromyography.Results: A 24.9% increase (p = 0.04) in the muscle oxidative capacity rate constant (k) occurred on WI 4 compared to baseline. No changes occurred in 40% MHE time to exhaustion or rate of fatigue or total work performed for the 50-repetition maximal isokinetic knee extension. The first quartile of deoxygenated hemoglobin concentration showed a 6 and 35% increase on WIs 3 and 5 (p = 0.026) with second quartile increases of 9 and 32% on WIs 3 and 5 (p = 0.049) during the 40% MHE testing when compared to WI 1.Conclusion: Our specific WI protocol resulted in no change to muscular endurance and oxygen kinetics in load bearing and non-load bearing muscles.

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