Decrease of vasoactive intestinal peptide, methionine-enkephalin, substance P and increase of neuropeptide Y immunoreactive nerve fibres in aganglionic colon of Hirschsprung's disease

Laet, Marc-Henri De; Dassonville, Martine; Steyaert, Henri; Vanderveken, Erwin; Lotstra, Françoise; Vierendeels, Godelieve; Przedborski, Serge; Vaudry, Hubert; Vanderhaeghen, Jean‐Jacques

doi:10.1016/0197-0186(89)90113-7

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…Our data also show that SP-immunoreactive nerve fibres are present in the myenteric ganglia of the rectum and the anal canal, in the circular and longitudinal muscle layers, as well as in the internal anal sphincter of 3-month old rats. The distribution of SP in the distal part of the large intestine is consistent with earlier findings in rats [20], guinea pigs [21] and humans [22]. Therefore, the presence and distribution of the SP-positive cell population in the recto-anal region indicates more similarities rather than species differences.…”

Section: Discussionsupporting

confidence: 81%

“…Our study clearly indicates degenerative alterations in SP-containing fibres in the terminal part of the rat colon which also support this finding. Also of interest, notably from a clinical point of view, is that disturbance and disruption of SP innervation of the aganglionic colon in Hirschsprung's disease, resulting in limited occurrence of immunoreactive fibres in the internal anal sphincter, are well documented [22,28]. Both of these findings suggest that, although agedependent SPergic neuronal loss does not cause dramatic changes in intestinal motility, it may be an underlying factor in the higher frequency of ano-rectal motility disorders encountered in the elderly [26].…”

Section: Discussionmentioning

confidence: 99%

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Age-related changes in substance P-immunoreactive nerve structures of the rat recto-anal region

et al. 2010

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AbstractThe recto-anal region is innervated by extrinsic and intrinsic nerves and a number of neuropeptides including substance P (SP) have been suggested to participate in the regulation of intestinal movements. We examined the age-related changes in the distribution of SP-immunoreactive nerve structures in the distal part of the rat large intestine. Using immunohistochemistry, the presence of SP was studied in fresh tissues from Wistar rats at different ages taken at three sampling sites, the distal rectum, anal canal and internal anal sphincter. In the 15-day old rats the myenteric plexus of the distal rectum and anal canal was well outlined by numerous SP-immunoreactive varicose nerve fibres encircling immunonegative perikarya. In the circular muscle layer, nerve fibres and small nerve bundles ran parallel to the muscle cells, while in the longitudinal muscle layer, only occasional nerve fibres were seen. At the level of the internal anal sphincter, no myenteric ganglia were present. Here, thin varicose fibers ran parallel to the smooth muscle cells. In the 3-month old rats, a larger number of intensely staining SP-immunoreactive nerve fibres were found and in the circular muscle layer, thicker nerve strands were observed. In the 26-month old rats, the density and staining intensity of SP-immunopositive nerve fibres in the myenteric plexus was lower than in the 3-month-old rats. Similar changes in the SP-immunostained fibres in the internal anal sphincter were observed. Degenerative alterations in SP-containing fibres during aging appear to play a role in ano-rectal motility and sphincter control.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Age-related changes in substance P-immunoreactive nerve structures of the rat recto-anal region

et al. 2010

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“…We cannot answer the question whether leuENK-reactive stubby neurons with and without additional SP reactivity are also functionally different or are rather chemically variable subtypes of a common population. Both ENK-reactive (Lolova et al 1986;De Laet et al 1989;Johanson et al 1991;Munakata et al 1997;Porter et al 1998) and SP-reactive neurons (Porter et al 1998;Neunlist et al 2003) were shown to participate in plastic changes in pathological conditions. ENK/ SP-co-reactive structures will have to be further evaluated in future studies, as will the leuENK-negative stubby neurons that were not further investigated here.…”

Section: Enkephalinergic Neurons In the Myenteric Plexus Of The Humanmentioning

confidence: 97%

“…Within pathologically altered gut samples derived from patients suffering from Hirschsprung's disease, intestinal neuronal dysplasia or slow transit constipation, ENK-immunoreactive nerve fibres were, in contrast to fibres displaying immunoreactivities for some other neuroactive substances, found to be reduced, especially in the circular muscle (Lolova et al 1986;De Laet et al 1989;Johanson et al 1991;Munakata et al 1997;Porter et al 1998). In segments obtained from patients with ulcerative colitis, Vento et al (2001) did not find differences in ENK-immunoreactive nerve elements in comparison to normal gut samples.…”

Section: Introductionmentioning

confidence: 96%

Morphology of enkephalin-immunoreactive myenteric neurons in the human gut

Brehmer

Lindig

Schrödl

et al. 2005

Histochem Cell Biol

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The aim of this study was the morphological and further chemical characterisation of neurons immunoreactive for leu-enkephalin (leuENK). Ten wholemounts of small and large intestinal segments from nine patients were immunohistochemically triple-stained for leuENK/neurofilament 200 (NF)/substance P (SP). Based on their simultaneous NF-reactivity and 3D reconstruction of single NF-reactive cells, 97.5% of leuENK-positive neurons displayed the appearance of stubby neurons: small somata; short, stubby dendrites and one axon. Of these leuENK-reactive stubby neurons, 91.3% did not display co-reactivity for SP whereas 8.7% were SP-co-reactive. As to their axonal projection pattern, 50.4% of the recorded leuENK stubby neurons had axons running orally whereas in 29.4% they ran anally; the directions of the remaining 20.2% could not be determined. No axons were seen to enter into secondary strands of the myenteric plexus. Somal area measurements revealed clearly smaller somata of leuENK-reactive stubby neurons (between 259+/-47 microm(2) and 487+/-113 microm(2)) than those of putative sensory type II neurons (between 700+/-217 microm(2) and 1,164+/-396 microm(2)). The ratio dendritic field area per somal area of leuENK-reactive stubby neurons was between 2.0 and 2.8 reflecting their short dendrites. Additionally, we estimated the proportion of leuENK-positive neurons in comparison to the putative whole myenteric neuron population in four leuENK/anti-Hu doublestained wholemounts. This proportion ranged between 5.9% and 8.3%. We suggest leuENK-reactive stubby neurons to be muscle motor neurons and/or ascending interneurons. Furthermore, we explain why we do not use the term "Dogiel type I neurons" for this population.

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Intestinal endocrine cells in Hirschsprung's disease

1990

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The aganglionic intestine in Hirschsprung's disease displays a severe neuronal derangement. The changes are particularly evident in the muscular innervation. In the gut the endocrine cells are among the cells known to be influenced by neurons. We have, therefore, examined the endocrine cells in ganglionic and aganglionic intestine using immunocytochemistry and immunochemistry. The endocrine cells were studied using antibodies against the neuroendocrine marker chromogranin A, the amine serotonin and the hormonal peptides somatostatin, glucagon/glicentin and peptide YY (PYY), thus covering virtually all endocrine cell types known to occur in this region. The PYY concentration in the mucosal layer was measured by radioimmunoassay. In ganglionic as well as in aganglionic intestine large populations of cells storing chromogranin A, serotonin, glucagon and PYY and a smaller population of somatostatin cells were seen. There was an increase in the density of these cells in the aganglionic intestine compared with ganglionic. The data indicate that the endocrine cell populations in the intestinal wall can be maintained despite severe derangements of the nerve supply.

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Decrease of vasoactive intestinal peptide, methionine-enkephalin, substance P and increase of neuropeptide Y immunoreactive nerve fibres in aganglionic colon of Hirschsprung's disease

Cited by 10 publications

References 21 publications

Age-related changes in substance P-immunoreactive nerve structures of the rat recto-anal region

Age-related changes in substance P-immunoreactive nerve structures of the rat recto-anal region

Morphology of enkephalin-immunoreactive myenteric neurons in the human gut

Intestinal endocrine cells in Hirschsprung's disease

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