Decreased collagen concentration in rat uterine implantation sites compared with non-implantation tissue at days 6-11 of pregnancy

Myers, DB; Clark, Dawn E.; Hurst, PR

doi:10.1071/rd9900607

Cited by 17 publications

(12 citation statements)

References 22 publications

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“…Hydroxyproline concentration was multiplied by a factor of (Woessner, 1962) (1977). Overall, collagen values were similar to those obtained by Myers et al (1990), again confirming that the effects of fixation were minimal.…”

Section: Introductionsupporting

confidence: 73%

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Collagen concentrations in dissected tissue compartments of rat uterus on Days 6, 7 and 8 of pregnancy

Clark¹,

Hurst²,

Myers³

et al. 1992

Reproduction

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Summary. Implantation and non-implantation sites were dissected into myometrial and stromal components; a decidual/embryonic region was obtained on Days 7 and 8 of pregnancy. The concentration of collagen (as a percentage of the dry weight of tissue), measured by hydroxyproline analysis, was significantly lower in the implantation regions than in the non-implant regions in all areas studied. The concentrations in the antimesometrial myometrium and stroma of the implantation region remained the same over the days studied. In contrast, the mesometrial collagen concentration in the implantation region declined from Day 6 to Day 8 of pregnancy. Collagen concentration was low within the decidual/embryonic tissue on Days 7 and 8 of pregnancy. Remodelling of collagen within the embryonic area appears to be an important feature of the uterine response to implantation in rats.

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Section: Introductionsupporting

confidence: 73%

“…Morphological studies have suggested a decline in collagen in the implantation area (Fainstat, 1963;O'Shea, 1983), but the extent of the decline was not measured. Myers et al (1990) (1967). Hydroxyproline concentration was multiplied by a factor of (Woessner, 1962) (1977).…”

Section: Introductionmentioning

confidence: 99%

Collagen concentrations in dissected tissue compartments of rat uterus on Days 6, 7 and 8 of pregnancy

Clark¹,

Hurst²,

Myers³

et al. 1992

Reproduction

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“…It has been suggested that implantation has evolved from a tissue response to a foreign body [1] and that the uterine response to an invading blastocyst or an artificial deciduogenic stimulus is similar to an inflammatory reaction [2]. The characteristics of the uterine response that are similar to inflammation include an elevated concentration of prostaglandins [3,4], leukotriene (LTB 4 and LTC 4 ) production [5], increased vascular permeability [6], oxygen radical production [7], tissue remodeling [8] including collagen degradation [9], and neutrophil infiltration [10]. Neutrophils have various capabilities that could modify the uterine responses to a blastocyst or deciduogenic stimulus.…”

Section: Introductionmentioning

confidence: 99%

An Investigation into the Role of Neutrophils in Decidualization and Early Pregnancy in the Rat1

Orlando-Mathur

Kennedy

1993

Biology of Reproduction

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Possible roles for neutrophils in the response of sensitized rat uteri to a deciduogenic stimulus were investigated. Ovariectomized rats were sensitized for the decidual cell reaction by the administration of estradiol and progesterone. Sesame oil was injected into one uterine horn as a deciduogenic stimulus on the equivalent of Day 5 of pseudopregnancy. Neutrophil numbers in the endometrium, as determined by microscopy after application of the stimulus, were dependent on time and stimulation. Very few neutrophils were found in the endometrium before stimulation. To determine whether neutrophils were involved in the uterine responses to a deciduogenic stimulus, rats were depleted of circulating neutrophils by prior treatment with either methotrexate (MET) or antineutrophil serum (ANS). The increase in endometrial vascular permeability, as quantified with [125I]-BSA 10 h after stimulation, was not significantly affected by MET or ANS treatment, nor was the extent of decidualization, as indicated by the weight of the uteri 5 days after stimulation. Endometrial neutrophil numbers were significantly lower in the MET- and ANS-treated animals compared to their respective controls. In the uterus, activity of myeloperoxidase, an enzyme found mainly in neutrophils, was significantly reduced in MET-treated animals. To investigate the role of neutrophils in blastocyst implantation, we treated rats with ANS early on Day 5 of pregnancy. On Day 6, neither the numbers of implantation sites nor their weights were affected by ANS treatment. ANS treatment on Day 5 of pregnancy did not attenuate the numbers and weights of placentae and fetuses on Days 19 and 20 of pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Thus, the more plausible explanation for increased capillary diameter may be that changes in vessel wall components (endothelial cells, basal lamina or both) may result in weakening that allows expansion of the capillaries. There is some evidence that collagen, a component of the vascular basal lamina, is being degraded at this early stage of pregnancy (Myers et al, 1990). In that study, collagen concentrations were significantly lower in implantation sites than in intersite tissue by day 6 of pregnancy.…”

Section: Discussionmentioning

confidence: 80%

In vivo microscopy of the subepithelial capillary plexus of the endometrium of rats during embryo implantation

Tawia¹,

Rogers²

1992

Reproduction

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Summary. Using in vivo microscopy we investigated endometrial microvascular events occurring on days 5 and 6 of pregnancy at the time of implantation. Blood flow through the endometrium was visualized using incident-light fluorescence microscopy and a video image was recorded for subsequent analysis. At 17:00 h on day 5 of pregnancy it was not possible to identify the impending implantation site from the in vivo appearance of the subepithelial capillary plexus. At 09:00 h on day 6 of pregnancy the embryo implantation site was recognized as an avascular area surrounded by large diameter vessels. These were highly susceptible to haemorrhage when handled. Capillaries closest to the embryo had the greatest diameters, averaging 18\m=.\5\ m=+-\2\m=.\5\g=m\m, and capillary diameters decreased to 7\m=.\5\m=+-\ 0\m=.\4 \g=m\mby 2000 \g=m\m from the embryo. It was also observed that blood flow through larger diameter vessels was sluggish with frequent reversals and stoppages. Leucocyte rolling and adhesion were also common features in these larger vessels. These data indicate that changes in capillary diameter occur in response to local signals associated with the implanting rat embryo. The embryonic or local endometrial signals that mediate these major microvascular changes remain to be elucidated.

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Decreased collagen concentration in rat uterine implantation sites compared with non-implantation tissue at days 6-11 of pregnancy

Cited by 17 publications

References 22 publications

Collagen concentrations in dissected tissue compartments of rat uterus on Days 6, 7 and 8 of pregnancy

Collagen concentrations in dissected tissue compartments of rat uterus on Days 6, 7 and 8 of pregnancy

An Investigation into the Role of Neutrophils in Decidualization and Early Pregnancy in the Rat1

In vivo microscopy of the subepithelial capillary plexus of the endometrium of rats during embryo implantation

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