Decreased CTLA4+ and Foxp3+ CD25highCD4+ Cells in Induced Sputum from Patients with Mild Atopic Asthma

Kawayama, Tomotaka; Matsunaga, Kazuko; Kaku, Yoichiro; Yamaguchi, Kyoko; Kinoshita, Takashi; O'Byrne, Paul M.; Hoshino, Tomoaki

doi:10.2332/allergolint.12-oa-0492

Cited by 25 publications

(22 citation statements)

References 34 publications

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“…In addition, the suppression of runx1 occurred at an earlier timepoint (1 day) than for foxp3 (4 days), an outcome that is consistent with the earlier requirement for runx1 in the regulatory T-cell pathway (Chatila, 2008). Reduced foxp3 expression has also been recently associated with asthma and immune-mediated skin diseases (Kawayama et al, 2013;Quaglino et al, 2013). Although the exact role of T H 17 cells in allergic disease is still unclear, available data suggest that this T-cell lineage may also contribute to the pathogenesis of atopic diseases such as asthma and contact dermatitis.…”

Section: Discussionmentioning

confidence: 54%

Expression kinetics of miRNA involved in dermal toluene 2,4-diisocyanate sensitization

Anderson

Beezhold

Lukomska

et al. 2013

Journal of Immunotoxicology

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Allergic disease is an important occupational health concern, with work-related asthma and allergic contact dermatitis being the most frequently diagnosed occupational illnesses. Diisocyanates, particularly toluene 2,4-diisocyanate (TDI), have been the leading cause of occupational asthma for many years. Understanding the mechanisms behind allergic disease is critical for treatment and prevention. Recently, the study of post-transcriptional regulation by microRNAs (miRNA) has shed light on mechanisms of allergic disease. The present studies report the expression of miRNA during the sensitization phase of an allergic response to TDI in a murine model. Female BALB/c mice were dermally exposed to TDI (0.1-15% [v/v]) or vehicle. RNA was isolated from superficial parotid lymph nodes at timepoints between 1 h and 15 days post-exposure and then miRNA expression was analyzed using array and real-time quantitative PCR analysis. Consistent changes in miRNA expression were identified for miR-21, miR-22, miR-27b, miR-31, miR-126, miR-155, miR-210, and miR-301a. Following TDI exposure, peak expression was observed by Day 4 for the majority of miRNA evaluated with trends in expression correlated to exposure concentration. Confirmed and predicted targets were identified using Diana-microT, miRanda, miRwalk, and Targetscan algorithms. Evaluation of mRNA expression of cytokine and transcription factor targets suggests that miRNA may have a central role early in TDI sensitization. Understanding the role of these miRNA and their specific mechanism of action in sensitization to TDI may provide pertinent information for the identification of other chemical sensitizers while also contributing to the treatment and prevention of allergic disease.

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Section: Discussionmentioning

confidence: 54%

Expression kinetics of miRNA involved in dermal toluene 2,4-diisocyanate sensitization

Anderson

Beezhold

Lukomska

et al. 2013

Journal of Immunotoxicology

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“…Our results [32,40] suggest that airway Tregs may be decreased, and that the decreased Tregs may be associated with increased airway inflammation in asthma. This inflammation may contribute to bronchoconstriction.…”

Section: Kinetics Of Tregs After Allergen Challenge In Adult Atopic Mmentioning

confidence: 69%

“…The induced sputum technique also can assess the effectiveness of newly developed agents [30,31]. The number of airway eosinophils in patients with mild asthma was significantly higher than non-asthmatic control subjects (control subjects) ( Figure 1A and 1B) [32]. The number of airway eosinophils was also increased in patients with asthma after allergen, but not methacholine (Mch) challenge ( Figure 1C) [33].…”

Section: Evaluation Of Airway Inflammation and Hyperresponsivenessmentioning

confidence: 99%

Role of Regulatory T cells in Airway Inflammation in Asthma

et al. 2017

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“…Peripheral blood collected from asthmatic showed decreased expression of CD4+CD25+ than in non-asthmatics (126). Other studies highlighted that Tregs were decreased in samples collected from the airways of atopic individuals (180). Shi et al, also observed that patients with moderate to severe asthma showed decreased number of CD4+CD25+ Tregs and had increased Th2 responses (181).…”

Section: Regulatory Mediatorsmentioning

confidence: 96%

Key mediators in the immunopathogenesis of allergic asthma

Hall

Agrawal

2014

International Immunopharmacology

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Asthma is described as a chronic inflammatory disorder of the conducting airways. It is characterized by reversible airway obstruction, eosinophil and Th2 infiltration, airway hyper-responsiveness and airway remodeling. Our findings to date have largely been dependent on work done using animal models, which have been instrumental in broadening our understanding of the mechanism of the disease. However, using animals to model a uniquely human disease is not without its drawbacks. This review aims to examine some of the key mediators and cells of allergic asthma learned from animal models and shed some light on emerging mediators in the pathogenesis allergic airway inflammation in acute and chronic asthma.

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Decreased CTLA4+ and Foxp3+ CD25highCD4+ Cells in Induced Sputum from Patients with Mild Atopic Asthma

Abstract: Airway, but not circulating, Tregs are decreased in mild atopic asthmatics, and are negatively correlated to an increase of airway eosinophilic inflammation and AHR.

Cited by 25 publications

References 34 publications

Expression kinetics of miRNA involved in dermal toluene 2,4-diisocyanate sensitization

Expression kinetics of miRNA involved in dermal toluene 2,4-diisocyanate sensitization

Role of Regulatory T cells in Airway Inflammation in Asthma

Key mediators in the immunopathogenesis of allergic asthma

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