Decreased expression of miR-126 correlates with metastatic recurrence of hepatocellular carcinoma

Chen, Hong‐Yuan; Miao, Ruizheng; Fan, Junwei; Han, Ziqiang; Wu, Junyi; Qiu, Guoqiang; Tang, Huamei; Peng, Zhihai

doi:10.1007/s10585-013-9569-6

Cited by 40 publications

(34 citation statements)

References 30 publications

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“…Sun, et al showed that miR-126, through targeting EGFL7, may suppress A549 cell proliferation in vitro , and inhibit tumor growth in vivo [9]. Chen et al revealed that decreased expression of miR-126 is associated with metastatic recurrence of HCC [19]. Consistent with previous studies, our results indicated that down-regulation of miR-126 was detected in the HCC tissues and associated with tumor size and liver cirrhosis.…”

Section: Discussionsupporting

confidence: 89%

MicroRNA-126 inhibits tumor proliferation and angiogenesis of hepatocellular carcinoma by down-regulating EGFL7 expression

Wang

et al. 2016

Oncotarget

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This study aims to explore the effects of microRNA-126 (miR-126) on tumor proliferation and angiogenesis of hepatocellular carcinoma (HCC) by targeting EGFL7. HCC tissues and adjacent normal tissues were obtained from 71 HCC patients. Immunohistochemistry (IHC) was conducted to detect expressions of EGFL7 and VEGF and the micro-vessel density (MVD). HCC cell lines were collected and assigned into the blank, miR-126 mimics, miR-126 inhibitors, miR-126 mimics negative control (NC), miR-126 inhibitors NC, si-EGFL7, and miR-126 inhibitors + si-EGFL7 groups. Expressions of miR-126 and EGFL7 mRNA were detected by qRT-PCR assay. The protein expressions of EGFL7 and VEGF were measured by Western blotting. MTT assay was used to measure the proliferation of HCC cells. Tumor xenograft model in nude mice was utilized to evaluate the influence of miR-126 on tumor growth. HCC tissues had higher miR-126 expression and lower EGFL7 mRNA expression than adjacent normal tissues. Compared with the blank, miR-126 mimic NC, miR-126 inhibitor NC and miR-126 inhibitors + si-EGFL7 groups, the protein expressions of EGFL7 and VEGF and cell proliferation were reduced in the miR-126 mimics and si-EGFL7 groups, while the opposite trend was found in the miR-126 inhibitors group. Compared with the blank and miR-126 inhibitors + siRNA-EGFL7 groups, tumor size, tumor weight, and MVD of transplanted tumors in nude mice were significantly reduced in the miR-126 mimics and siRNA-EGFL7 groups, while the opposite trend was found in the miR-126 inhibitors group. In conclusion, miR-126 could inhibit tumor proliferation and angiogenesis of HCC by down-regulating EGFL7 expression.

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Section: Discussionsupporting

confidence: 89%

MicroRNA-126 inhibits tumor proliferation and angiogenesis of hepatocellular carcinoma by down-regulating EGFL7 expression

Wang

et al. 2016

Oncotarget

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“…Therefore, low expression of miR-194 in the preexisting advanced adenoma could be a predictor for adenoma recurrence. This hypothesis was supported by a clinical study performed by Chen and colleagues, in which the low expression of miR-126 in liver tissues was significantly associated with tumor recurrence in patients with HCC who underwent liver transplantation (27). Certainly, we also should investigate whether the expression of miR-194 is downregulated in the normal colon mucosa and recurrent adenoma of the patients.…”

Section: Discussionmentioning

confidence: 72%

miR-194 as a Predictor for Adenoma Recurrence in Patients with Advanced Colorectal Adenoma after Polypectomy

Wang

Ren

Zheng

et al. 2014

Cancer Prevention Research

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microRNAs (miRNA) are promising predictors in colorectal cancer (CRC). We investigated whether miRNAs could predict adenoma recurrence in patients with advanced colorectal adenoma (ACRA) after polypectomy. miRNA expression profiling was performed by miRNA microarray to identify recurrencerelated miRNAs. Candidate miRNAs extracted from formalin-fixed paraffin-embedded blocks of patients with ACRA were measured using real-time PCR. Logistic regression analysis was conducted to investigate whether validated miRNA expression profiles were independent from other known adenoma recurrence risk factors. The prognostic values of six miRNAs and three independent risk factors were assessed by the area under the receiver operating characteristic (ROC) curve analysis. The expressions of six candidate miRNAs were significantly decreased from levels in normal colorectal tissue compared with ARCA with adenoma recurrence (RACRA) in this retrospective cohort. However, only miRNA (miR)-194 emerged as a practical predictor. The sensitivity and specificity of miR-194 as a predictor were 71.0% and 78.0%, respectively, at a cutoff value of 0.1311 in the retrospective cohort. Sensitivity and specificity were 76.1% and 77.2%, respectively, in the prospective cohort using the same cutoff value. Low expression levels of miR-194, adenoma size !2 cm, and !3 adenomas were independent risk factors for adenoma recurrence. Moreover, low expression of miR-194 was a better predictor of adenoma recurrence than the adenoma size and numbers according to ROC curve analysis. miR-194 may be an independent predictor for adenoma recurrence in patients with ACRA after polypectomy. Cancer Prev Res; 7(6); 607-16. Ó2014 AACR.

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“…For example, decreased expression of miR-126 in HCC has been correlated with HCC recurrence and poor survival after liver transplantation [30]. In another study, a predictive score combining the expression levels of miR-214, miR-3187 and the Milan criteria was proposed to stratify patients according to their risk for recurrence of HCC and death after liver transplantation [31].…”

Section: Discussionmentioning

confidence: 99%

High expression of micro RNA-135A in hepatocellular carcinoma is associated with recurrence within 12 months after resection

et al. 2017

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BackgroundHepatocellular carcinoma has a dismal prognosis due to recurrence rates of up to 70% after curative resection. Early recurrence is driven by synchronous microscopic intrahepatic metastases. The predictive value of histological parameters is discussed controversially and adjuvant therapy is not established. The aim of this study was to identify patients at high risk for early intrahepatic recurrence by expression profiling of selected micro RNAs.MethodsIn 52 patients undergoing HCC resection between 2011 and 2014, liver and tumor tissue was collected during surgery. Twelve patients with incomplete data regarding HCC recurrence, secondary liver transplantation, or perioperative death were excluded, leaving 40 patients with early recurrence <12 months (R+) or without recurrence for >24 months (R-) to compare grading, T, L, V, and R status. If tissue quality permitted, micro RNAs were measured in HCC and liver tissue.ResultsTen women and 30 men (64.0 ± 10.2 years) were analyzed. R+ occurred in 29 patients 6.2 ± 4.5 months after resection. Surveillance of R- was 26.2 ± 5.2 months. High intratumoral expression of miR-135a was associated with high risk of recurrence (HR = 4.2, p = 0.024, time to recurrence 8.8 ± 2.0 vs. 24.8 ± 4.4 months in patients with low miR-135a expression). As expected, T3 status was correlated with early recurrence, while other histological parameters and expression of miR-21, miR-122, and miR-125a did not.ConclusionsWe show a significant association between high expression of miR-135a and early HCC recurrence. Therefore, high intratumoral miR-135a expression might serve as a novel biomarker to identify patients urgently requiring adjuvant therapy post resection.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-017-3053-7) contains supplementary material, which is available to authorized users.

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Decreased expression of miR-126 correlates with metastatic recurrence of hepatocellular carcinoma

Cited by 40 publications

References 30 publications

MicroRNA-126 inhibits tumor proliferation and angiogenesis of hepatocellular carcinoma by down-regulating EGFL7 expression

MicroRNA-126 inhibits tumor proliferation and angiogenesis of hepatocellular carcinoma by down-regulating EGFL7 expression

miR-194 as a Predictor for Adenoma Recurrence in Patients with Advanced Colorectal Adenoma after Polypectomy

High expression of micro RNA-135A in hepatocellular carcinoma is associated with recurrence within 12 months after resection

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